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Bone Abstracts (2013) 1 PP197 | DOI: 10.1530/boneabs.1.PP197

ECTS2013 Poster Presentations Cell biology: osteoblasts and bone formation (50 abstracts)

EGF suppresses BMP2-induced osteogenic differentiation through the up-regulation of Smurf1 expression

Hye-Lim Lee , Kyung Mi Woo , Hyun-Mo Ryoo , Gwan-Shik Kim & Jeong-Hwa Baek


Seoul National University School of Dentistry, Seoul, Republic of Korea.


Although EGF has been known to inhibit osteoblast differentiation, its molecular mechanism has not been clearly elucidated. Smurf1 acts as a negative regulator of BMP signaling by inducing ubiquitination and proteasomal degradation of BMP type I receptor and R-Smads. In this study, we investigated the effect of EGF on the expression of Smurf1 and the role of Smurf1 in EGF-induced inhibition of BMP2-induced osteogenesis. EGF increased Smurf1 expression which was blocked by treatment with a specific inhibitor of EGFR tyrosine kinase, JNK or ERK. Reporter assay using the constructs containing the sequence of Smurf1 promoter, demonstrated that AP-1 and Runx2 are the transcription factors activated by JNK and ERK, respectively. EGF treatment or Smurf1 overexpression suppressed BMP2-induced expression of osteogenic marker genes, whereas knockdown of Smurf1 partially rescued the expression of these genes in EGF-treated cells. Taken together, these results suggest that the JNK-c-Jun and the ERK-Runx2 signaling pathways play an important role in the regulation of Smurf1 expression by EGF and that Smurf1 partially mediates the inhibitory effect of EGF on osteogenic differentiation.

Volume 1

European Calcified Tissue Society Congress 2013

Lisbon, Portugal
18 May 2013 - 22 May 2013

European Calcified Tissue Society 

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