Searchable abstracts of presentations at key conferences on calcified tissues
Bone Abstracts (2013) 1 PP370 | DOI: 10.1530/boneabs.1.PP370

ECTS2013 Poster Presentations Osteoporosis: pathophysiology and epidemiology (49 abstracts)

Up-regulation of inhibitors of DNA binding/differentiation gene during alendronate-induced osteoblast differentiation

Heung Yeol Kim 1 & Hoon Choi 2


1Kosin University, Busan, Republic of Korea; 2Inje University, Seoul, Republic of Korea.


Aim: Alendronate enhances bone morphogenetic proteins (BMP)-mediated osteoblast differentiation. A balanced regulation of inhibitors of DNA binding/differentiation (Ids) plays an important role in BMP-induced osteoblast differentiation. However, there are no studies on the possible roles of Id genes in alendronate-induced osteoblast differentiation. This study investigated the effect of alendronate on the expression of Id genes in osteoblast differentiation.

Methods: C2C12 cells were treated with alendronate for various concentrations and time periods. For evaluation of alendronate-induced osteoblast differentiation in C2C12 cells, alkaline phosphatase (ALP) activity was measured. The expression of osteoblast differentiation markers such as ALP, type 1 collagen (Col 1), and osteocalcin (OCN), and the expression of Id-1 and Id-2 were measured by RT-PCR. In order to understand the mechanism underlying the regulation of Id genes, the promoter region of the Id-1 gene was identified. Database analysis of the promoter region for Id-1 using known consensus sequences identified several putative response elements, including CCAAT/enhancer-binding protein β (C/EBPβ).

Results: Alendronate treatment significantly increased not only ALP activity but also expression of ALP, Col 1, and OCN, Id-1 and Id-2. C/EBPβ and alendronate cooperatively increased the promoter activity and expression of Id-1.

Volume 1

European Calcified Tissue Society Congress 2013

Lisbon, Portugal
18 May 2013 - 22 May 2013

European Calcified Tissue Society 

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