Searchable abstracts of presentations at key conferences on calcified tissues
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European Calcified Tissue Society Congress 2013

Lisbon, Portugal
18 May 2013 - 22 May 2013

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European Calcified Tissue Society Congress 2013, 18 - 22 May 2013; Lisbon, Portugal

Poster Presentations

Arthritis and other joint diseases: translational and clinical

ba0001pp13 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Clinical and histomorphometrical assessment of bone quality in hip osteoarthritis and osteoporosis

Feola Maurizio , Rao Cecilia , Celi Monica , Gasbarra Elena , Tarantino Umberto

Osteoarthritis (OA) and osteoporosis (OP) are two diseases characterized by the alteration of bone quality, that affect mainly elderly people reducing their quality of life. Although an inverse relationship between has been shown by some studies, other reports supported the co-existence of these pathologies. In this study we combined clinical and structural features to clarify the relationship between OA and OP.Among all the patients who underwent a tota...

ba0001pp14 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Prophylaxis of gout flare with colchicine and vitamin C

Monov Simeon , Monova Daniela , Rashkov Rasho

Background: The incidence and prevalence of gout have markedly increased over the last few decades in keeping with the rise in prevalence of obesity and metabolic syndrome. The management of gout in patients with associated metabolic syndrome and comorbid illnesses such as renal impairment was difficult because of limited treatment options. Recent efficacy and safety data favour lower over higher doses of colchicine, and oral corticosteroids over non-steroidal anti-inflammator...

ba0001pp15 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Cartilage intermediate layer protein is produced in synovial membrane of osteoarthritic joint and upregulated in osteoarthritis associated fibrosis

Kerna Irina , Kisand Kalle , Tamm Ann , Tamm Agu

Introduction: Cartilage intermediate layer protein (CILP) is a promising marker of osteoarthritis (OA). CILP is an extracellular matrix glycoprotein, which is produced by cartilage chondrocytes. Still, there are data that CILP could be found also in other tissues. We aimed to investigate the expression level of CILP mRNA in the synovial membrane and evaluated the associations of CILP expression with traits of radiographic knee OA (rKOA) and features of histol...

ba0001pp16 | Arthritis and other joint diseases: translational and clinical | ECTS2013

U-C2C in estonian early knee OA cohort: progressive and non-progressive cases

Tamm Agu , Tamm Ann , Kumm Jaanika , Vija Maret , Lintrop Mare

Biomarkers are required to detect early OA for intervention and to monitor disease progression. A collagen type II neoepitope C2C was developed for these purposes. The aims of the study to test: i) the biomarker’s ability to differentiate osteo-arthritis (OA) patients with and without structural changes and ii) possible contribution of progression of the OA.Material and methods: We investigated 159 knee OA patients aged 36–62 (mean 50) years. F...

ba0001pp17 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Bone is the main target of activation of Canonical Wnt pathway in osteoarthritis

Funck-Brentano Thomas , Bouaziz Wafa , Geoffroy Valerie , Hannouche Didier , Marty Caroline , Hay Eric , Cohen-Solal Martine

Objective: Wnt/β-catenin pathway is a main regulator of bone remodeling, but might be inhibited in cartilage in osteoarthritis (OA). We here investigated the effect of mechanical loading in Wnt activation and the expression of Wnt antagonists in the joint tissues.Methods: Topgal mice were used. Mice underwent partial meniscectomy (Mnx) and sacrificed at 4, 6, and 9 weeks. Dissected knees were scanned by microCT and then prepared for cryosectioning t...

ba0001pp18 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Milk fat globule-epidermal growth factor 8 is a critical determinant of bone mass and alters the course of inflammation in arthritis

Sinningen Kathrin , Thiele Sylvia , Grossklaus Sylvia , Udey Mark , Hofbauer Lorenz C , Chavakis Triantafyllos , Rauner Martina

Milk fat globule-epidermal growth factor 8 (MFG-E8) is a glycoprotein that controls the engulfment of apoptotic cells and exerts anti-inflammatory effects. It has been implicated in the pathogenesis of several diseases, but its role in the bone microenvironment is still unknown. Here we tested the hypothesis that MFG-E8 also regulates bone metabolism and the development of arthritis.MFG-E8 expression was detected in mouse bones and primary murine osteobl...

ba0001pp19 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Vitamin K2 administration is associated with decreased disease activity in patients with rheumatoid arthritis

Ebina Kosuke , Morimoto Tokimitsu , Shi Kenrin , Kaneshiro Shoichi , Koizumi Kota , Hirao Makoto , Hashimoto Jun , Yoshikawa Hideki

Objectives: Recent studies have demonstrated that vitamin K2 (VitK2) induces apoptosis of not only osteoclasts but also rheumatoid arthritis (RA) synovial cells in vitro, while its clinical effect on disease activity of RA remains unknown.Methods: 158 female RA patients (age 62.5 years, duration of disease 14.9 years) who had not been treated with warfarin, biologics, or teriparatide were enrolled in this study. VitK2 (45 mg/day) was administered orally ...

ba0001pp20 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Immunological profile of 110 rheumatoid arthritis

Nassar Kawtar , Janani Saadia , Rachidi Wafaa , Mkinsi Ouafaa

Introduction: Rheumatoid arthritis is the most common inflammatory arthritis. It is also an autoimmune disease. It’s immunological profile is typical and often correlated with clinical presentation. If acticorps anti-CCP and rheumatoid factor are part of the diagnostic criteria. Anti-nuclear antibody are found in 15–40%.Objectives: Studying the immunological profile in 110 patients treated for rheumatoid arthritis.Materie...

ba0001pp21 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Monosodium urate crystals inhibit tenocyte viability and function: implications for periarticular involvement in chronic gout

Chhana Ashika , Callon Karen , Dray Michael , Pool Bregina , Naot Dorit , Gamble Greg , Coleman Brendan , McQueen Fiona , Cornish Jillian , Dalbeth Nicola

Background: In patients with gout, urate deposition has been observed both adjacent to and within tendons, suggesting that monosodium urate monohydrate (MSU) crystals are likely to be in direct contact with tenocytes, the stromal cells of tendons. The aim of this study was to determine the effects of MSU crystals on tenocyte viability and function.Methods: Cultures of primary rat tenocytes were prepared from Wistar rat tails. Primary human tenocytes were...

ba0001pp22 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Generalized long term bone loss in early rheumatoid arthritis in the biologic treatment era: a 10-year prospective observational study

Proven Anne , Helgetveit Knut , Haugeberg Glenn

Background: Several short-term studies have been performed in rheumatoid arthritis (RA) reporting a rapid rate of generalized bone loss. Aggressive anti-inflammatory treatment with biologic disease modifying anti-rheumatic drugs (DMARDs) has been shown to reduce the rate of bone loss1. There is a lack of long term follow-up studies.Objectives: To study 10-year changes in generalized bone loss in early RA patients in the biologic treatment era....

ba0001pp23 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Ultrasound carotid plaque morphology in rheumatoid arthritis women without previous cardiovascular events

Castro Alice , Carmona-Fernandes Diana , Santos Maria Jose , Mendes-Pedro Luis , Canhao Helena , Fonseca Joao Eurico

Introduction: In rheumatoid arthritis (RA) patients subclinical atherosclerosis and cardiovascular events (CV) occur more frequently and at younger ages than in the general population. Previous data suggest that heterogeneous plaques on USA are more unstable and frequently contain a higher amount of lipids and which make them hypoechoic and had higher potential for embolization and thrombosis. The aim of our work was to estimate the prevalence and the ultrasound morphology of ...

ba0001pp24 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Bone mineral density changes after total knee replacement in women over the age of 65

Kim Deog Yoon

Objective: We analyze changes of spine BMD and hip BMD after total knee replacement (TKR) according to osteoporosis medication, bilaterality of operation site, degree of obesity, degree of knee function and osteoporosis diagnosis at operation in female patients above 65 years old, who received TKR due to severe knee osteoarthritis,Method: 52 patients who checked 1 year follow-up BMD were enrolled. Patients were checked the spine and hip BMD at pre-operat...

ba0001pp25 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Rehabilitation of patients with early rheumatoid arthritis, including cryotherapy, physical exercises, occupational therapy, orthoses, and therapeutic education

Orlova Evgeniya , Karateev Dmitry , Kochetkov Andrey , Denisov Lev

Introduction: The evidence for the effectiveness of early rehabilitation of patients with rheumatoid arthritis (RA) is scanty. The aim of the study is to evaluate the efficiency of rehabilitation program for patients with early RA.Methods: 34 study group patients with early RA underwent 6-month rehabilitation (hospital stage: 15-min local air cryotherapy (−;60 °C, Criojet Air C600) for hand, knee or ankle joints, 45-min therapeutic exercises u...

ba0001pp26 | Arthritis and other joint diseases: translational and clinical | ECTS2013

The femoral neck fractures in patients with rheumatoid arthritis

Podvorotova Marina , Dydykina Irina

The femoral neck fractures (FNF) are one of the most common non-traumatic fractures in elderly people. Frequently the patients with fractures of this localization need surgical treatment and long-term rehabilitation. It’s widely known that FNF in patients with rheumatoid arthritis (RA) occur more often than in the population.Background: To determine the frequency of FNF in patients with RA and to characterize the patients with RA and with FNF at the...

ba0001pp27 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Sclerostin/MEPE axis in OA: lessons from long bone development

Staines Katherine , Poulet Blandine , Farquharson Colin , Pitsillides Andrew

The re-initiation of developmental processes in osteoarthritis (OA) has emerged with similarities to endochondral ossification; responsible for long bone development. We aimed to establish the role of the Wnt inhibitor, sclerostin in endochondral ossification, and its relationship with MEPE, a calcification inhibitor with potential downstream functions. Knee joints from male Str/ort (spontaneous OA) and age-matched CBA control mice were analysed at 8, 18, and 40+ weeks of age ...

ba0001pp28 | Arthritis and other joint diseases: translational and clinical | ECTS2013

The glutamate receptor antagonist NBQX alleviates inflammation, pathology and gait abnormalities in rat antigen induced arthritis

Bonnet Cleo , Williams Anwen , Gilbert Sophie , Harvey Ann , Evans Bronwen , Mason Deborah

Objectives: Synovial fluid glutamate concentrations increase in various arthritides. Activation of kainate (KA) and AMPA glutamate receptors (GluRs) increase interleukin 6 (IL6) release and cause arthritic pain respectively. GluR antagonists represent potential peripheral treatments for inflammatory arthritis and inflammatory mechanisms that contribute to osteoarthritis (OA). We hypothesised that AMPA and KA GluRs are expressed in arthritic joint tissues and that peripheral ad...

ba0001pp29 | Arthritis and other joint diseases: translational and clinical | ECTS2013

A deletion mutation of the gene P58IPK, the cellular inhibitor of PKR and PERK, results in a degenerative joint phenotype in mice

Gilbert Sophie , Nowell Mari , Bonnet Cleo , Ladiges Warren , Morton John , Duance Vic , Mason Debbie

Objective: The protein kinases, PKR, and PERK have been implicated in pro-inflammatory cytokine-mediated cartilage degradation in vitro and endoplasmic reticulum stress-induced arthritis respectively. The objective of this study was to establish whether loss of P58IPK, an inhibitor of PKR and PERK, results in a degenerative joint phenotype in vivo.Methods: Sections of knee joints from P58IPK-null and wild-type mice...

ba0001pp30 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Changes and comparison of bone metabolism, bone mineral density, MRI in early rheumatoid arthritis

Vershhynina Diana , Ryzhyk Varerij , Mikhalchenko Olena , Golovach Iryna , Dudij Peter , Semeniv Igor , Shevchuk Olga

The problem of early diagnosis of rheumatoid arthritis remains an important area of research in rheumatology. We investigated changes in bone metabolism, bone mineral density in early rheumatoid arthritis (ERA; up to 12 months). Data were compared with changes in the MRI study of the dominant hand.We observed 24 patients with ERA, the average age – 33.6±5.7 years. The men were 6 (25%), women – 18 (75%). Bone mineral density (BMD) was deter...