Searchable abstracts of presentations at key conferences on calcified tissues
Previous issue | Volume 3 | ECTS2014 | Next issue

European Calcified Tissue Society Congress 2014

Prague, Czech Republic
17 May 2014 - 20 May 2014

Card image cap
European Calcified Tissue Society Annual Congress, 17 - 20 May 2014; Prague, Czech Republic

Poster Presentations

Cell biology: osteoblasts and bone formation

ba0003pp104 | Cell biology: osteoblasts and bone formation | ECTS2014

Overexpression of Sp7 in osteoblasts inhibits osteoblast maturation but enhances angiogenesis in bone

Komori Hisato , Miyazaki Toshihiro , Yoshida Carolina A , Komori Toshihisa

Sp7 and Runx2 are essential transcription factors for osteoblast differentiation at an early stage, and Runx2 inhibits osteoblast maturation at a late stage. However, the functions of Sp7 in bone development are not fully elucidated. Thus, we pursued the functions of Sp7 in bone development by generating osteoblast-specific Sp7 transgenic (tg) mice using 2.3 kb Col1a1 promoter. Sp7 tg mice showed osteopenia and woven-bone like structure in the cortic...

ba0003pp105 | Cell biology: osteoblasts and bone formation | ECTS2014

Reduced bone formation and increased adiposity with insulin resistance in interleukin-11 deficient mice

Dong Bingzi , Kondo Takeshi , Ohnishi Yukiyo , Endo Itsuro , Abe Masahiro , Aizawa Shinichi , Sakaue Hiroshi , Matsumoto Toshio

We previously reported that interleukin (IL)-11 plays an important role in the mechanical stress and PTH-induced stimulation of osteoblast differentiation and bone formation. Mechanical stress and PTH enhance IL-11 gene promoter activity and increase IL-11 expression. Increased IL-11 down-regulates dikkopf 1/2 (Dkk1/2) expression and stimulates canonical Wnt signaling, which enhances osteoblast differentiation. The present study was undertaken to clarify the physiological role...

ba0003pp106 | Cell biology: osteoblasts and bone formation | ECTS2014

Downregulation of Smurf1 is involved in odontoblast differentiation induced by histone deacetylase inhibitors

Kwon Arang , Woo Kyung Mi , Ryoo Hyun-Mo , Baek Jeong-Hwa

Smurf1 is an E3 ubiquitin ligase for Smad1/5, Runx2 and MEKK2 and plays a role as a negative regulator for osteoblast differentiation and bone formation. However, the role of Smurf1 in odontoblast differentiation has not been elucidated. Previously, we reported that histone deacetylase inhibitors (HDACi) enhance odontoblast differentiation through the upregulation of Nfic expression. In this study, we investigated the regulatory effect of HDACi on Smurf1 expression and the rol...

ba0003pp107 | Cell biology: osteoblasts and bone formation | ECTS2014

Osteoblast-specific overexpression of amphiregulin leads to transient increase in cancellous bone mass in mice

Vaidya Mithila , Lehner Diana , Handschuh Stephan , Jay Freya , Schneider Marlon R , Erben Reinhold G

It is well known that the epidermal growth factor receptor (EGFR), its ligands, and the structurally related receptor ERBB2/neu are expressed in skeletal cells. However, the functions of EGFR ligands in bone cells remain poorly defined. In this study, we employed a transgenic mouse line overexpressing the EGFR ligand amphiregulin (AREG) specifically in osteoblasts under the α1(I)-collagen promoter. AREG-tg mice did not show changes in body weight or gross phenotype. Compa...

ba0003pp108 | Cell biology: osteoblasts and bone formation | ECTS2014

Collagen XIII in bone homeostasis

Koivunen Jarkko , Kemppainen Antti , Haronen Heli , Finnila Mikko , Miinalainen Ilkka , Heikkinen Anne , Sormunen Raija , Pihlajaniemi Taina

Collagen XIII is a conserved transmembrane collagen with wide distribution in various tissues. It can be enzymatically cleaved to form a soluble bioactive molecule with relevance to cell proliferation, migration and adhesion. Collagen XIII overexpression in mice causes a massive bone overgrowth with no defects in early skeletal development. The bone phenotype of Col13a1OE mice is most apparent in long tubular bones but also present in calvariae. Our findings show th...

ba0003pp109 | Cell biology: osteoblasts and bone formation | ECTS2014

Advanced glycation end products inhibit the mineralization of marrow stromal cells by binding the receptor for AGEs and increasing TGF-β expression and secretion

Yamaguchi Toru , Notsu Masakazu , Okazaki Kyoko , Tanaka Ken-ichiro , Ogawa Noriko , Kanazawa Ippei , Sugimoto Toshitsugu

We and others have recently showed that patients with type 2 diabetes mellitus (T2DM) have a higher risk for fracture, although they have normal or slightly higher bone mineral density (BMD). These findings suggest that bone fragility in T2DM, which is not defined by BMD, may contribute to fracture risk. Hyperglycemia accelerates the formation of advanced glycation end products (AGEs) and causes diabetic complications. AGEs including pentosidine are also known to cause bone fr...

ba0003pp110 | Cell biology: osteoblasts and bone formation | ECTS2014

Age-dependent loss of microvesicular galectin-3 and its consequences on bone formation in vitro and in vivo

Weilner Sylvia , Heimel Patrick , Keider Verena , Schraml Elisabeth , Weisz Florian , Chiang DML , Grillari-Voglauer Regina , Redl Heinz , Chen HY , Grillari Johannes

Introduction: Mesenchymal stem cells (MSCs) counteract the decline of physiologic functions but their regenerative power decreases with age. In particular osteogenic differentiation capacity of MSCs has been shown to decrease with age thereby contributing to slowed down bone formation and osteoporosis. While much is known about cellular aging of MSCs, little is known about extrinsic factors influencing their functionality. Here we set out to identify circulating factors of the...

ba0003pp111 | Cell biology: osteoblasts and bone formation | ECTS2014

Osteopontin as a novel substrate for proprotein convertase 5/6 (PCSK5) in bone

Hoac Betty , Susan-Resiga Delia , Essalmani Rachid , Marcinkiweicz Edwidge , Barros Nilana , Seidah Nabil , McKee Marc

Proprotein convertase PC5/6 (Pcsk5) is expressed in mouse bone and Pcsk5 epiblast-specific conditional knockout mice have a bone phenotype displaying small size, delayed ossification and additional thoracic segments and ribs. Some features are attributed to growth and developmental factor 11 (GDF11) – a known substrate for PC5/6 – while the delayed mineralization has yet to be explained. Osteopontin (OPN) is a bone matrix protein with roles in miner...

ba0003pp112 | Cell biology: osteoblasts and bone formation | ECTS2014

Levels of circulating vesicular microRNA-31 increase with age as well as in the case of osteoporosis and inhibit osteogenic differentiation capacity of mesenchymal stem cells

Weilner Sylvia , Schraml Elisabeth , Wassermann Klemens , Wieser Matthias , Messner Paul , Schneider Karl , Micutkova Lucia , Fortschegger Klaus , Maier Andrea B. , Westendorp Rudi , Resch Heinrich , Wolbank Susanne , Redl Heinz , Jansen-Durr Pidder , Pietschmann Peter , Grillari-Voglauer Regina , Grillari Johannes

Aging is a complex process that results in the decline of physiologic functions due to accumulation of damage in cells and tissues. Mesenchymal stem cells (MSCs) counteract this decline but their regeneration capacity decreases with age. In particular osteogenic differentiation potential of MSCs has been shown to decrease with age thereby contributing to slowed down bone formation and osteoporosis. While much is known about cellular aging of MSCs, little is known about factors...

ba0003pp113 | Cell biology: osteoblasts and bone formation | ECTS2014

Bone-anabolic effects of sulforaphane, a naturally occurring isothiocyanate on bone metabolism

Thaler Roman , Dudakovic Amel , Rucci Nadia , Maurizi Antonio , Sturmlechner Ines , Spitzer Silvia , Rumpler Monika , Van Wijnen Andre J , Teti Anna , Klaushofer Klaus , Varga Franz

Few drugs generate bone-stimulatory effects via epigenetic mechanisms. Modulation of CpG-residues hydroxymethylation in gene-promoters of key osteoblast-related factors (e.g., DLX5) induces their expression and increases osteoblast differentiation in vitro. The chemical properties of sulforaphane (SFN), a natural compound abundantly present in cruciferous vegetables like broccoli, suggest that it may have similar molecular and biological effects. Previous stu...

ba0003pp114 | Cell biology: osteoblasts and bone formation | ECTS2014

N-linked glycosylation as a critical mechanism of PTH-resistance in osteoblasts in high glucose conditions

Picke Ann-Kristin , Hamann Christine , Rauner Martina , Hofbauer Lorenz C.

Type 2 diabetes mellitus impairs bone quality and increases fracture risk. We showed that diabetic ZDF rats have low bone mass due to impaired osteoblastogenesis, which can be partially reversed with an intermittent parathyroid hormone 1–84 (PTH) therapy. It remains unclear, why PTH treatment does not fully restore osteoblast (OB) function in diabetic conditions. Here, we tested if high glucose (HG) conditions lead to a partial PTH resistance in osteoblasts. Pre-osteoblas...

ba0003pp115 | Cell biology: osteoblasts and bone formation | ECTS2014

Epigenetic modifications and canonical WNT signaling enable direct programming of non-osteogenic cells into osteoblasts

Cho Young-Dan , Yoon Won-Joon , Woo Kyung-Mi , Baek Jeong-Hwa , Lee Gene , van Wijnen Andre J , Ryoo Hyun-Mo

Mesenchymal cells alter and retain their phenotype during skeletal development through activation or suppression of signaling pathways. For example, we have shown that Wnt3a only stimulates osteoblast differentiation in cells with intrinsic osteogenic potential (e.g., MC3T3-E1 pre-osteoblasts) and not in fat cell precursors or fibroblasts (respectively, 3T3-L1 pre-adipocytes or NIH3T3 fibroblasts). Wnt3a promotes osteogenesis in part by stimulating autocrine production of the ...

ba0003pp116 | Cell biology: osteoblasts and bone formation | ECTS2014

Inhibitory effect of GH on the adipogenic commitment of mesenchymal stromal cells derived from human trabecular bone

Bolamperti Simona , Spinello Alice , Sauer Aisha , GianLuigi Moro , Ometti Marco , Fraschini Gianfranco , Guidobono Francesca , Rubinacci Alessandro , Villa Isabella

Besides its well known effect on longitudinal bone growth, GH plays a role in the maintenance of adult bone mass. As aging progresses GH levels decline, bone mass decreases and mesenchymal precursors show a reduced osteogenic differentiation capacity resulting in an increase in bone marrow adipocytes. We investigated the effect of GH on MSC differentiation and the possible involvement of microRNA in this process. Human MSC derived from trabecular specimens, waste material of o...

ba0003pp117 | Cell biology: osteoblasts and bone formation | ECTS2014

EGF inhibits Wnt/β-catenin-induced osteoblast differentiation in a Smurf1-dependent manner

Kwon Arang , Boonanantanasarn Kanitsak , Lee Hye-Lim , Woo Kyung Mi , Ryoo Hyun-Mo , Kim Gwan-Shik , Baek Jeong-Hwa

BMPs and canonical Wnts are the representative developmental signals that enhance osteoblast differentiation and bone formation. Previously, we demonstrated that EGF inhibits BMP2-induced osteoblast differentiation through the induction of Smurf1 expression. However, the regulatory role of EGF in Wnt/β-catenin-induced osteoblast differentiation has not been elucidated. In this study, we investigated the effect of EGF on Wnt/β-catenin signaling-induced osteoblast diff...

ba0003pp118 | Cell biology: osteoblasts and bone formation | ECTS2014

HBO increased osteogenic differentiation of MSCs via regulating Wnt processing, secretion, and signaling

Yang Chuen-Yung , Lin Song-Shu , Ueng Steve WN , Niu Chi-Chien , Yuan Li-Jen , Chen Wen-Jer

Background: Autocrine and paracrine Wnt signaling operates in stem cell populations and regulates mesenchymal lineage specification. In Wnt producing cells, Wntless (GPR177) supports the transport of Wnt from the trans-Golgi network (TGN) to the cell surface in vesicles, from which Wnt is then released. The retromer complex (VPS35) interacts with Wntless and retrieves Wntless from endosomes back to TGN, thereby maintaining the normal levels of Wntless protein. V-ATPases-driven...

ba0003pp119 | Cell biology: osteoblasts and bone formation | ECTS2014

Effect of osteoporosis in the transcriptional profile of osteoblastic cells from bone marrow and calvaria of ovariectomized rats

Semeghini Mayara Sgarbi , de Azevedo Fernanda Grilo , de Assis Amanda Freire , Siessere Selma , Beloti Marcio Mateus , Rosa Adalberto Luiz , Fernandes Roger Rodrigo , Passos Geraldo Aleixo , Prado Karina Fittipaldi Bombonato

Changes in the ability of self-renewal and differentiation of mesenchymal stem cells may be affected by osteoporosis. The aim of this investigation was to compare the transcriptional profile of mRNAs and miRNAs of osteoblastic cells from calvaria and bone marrow of female rats after ovariectomy. Following the approval by ethics committee, 18 wistar rats were divided into control (sham) and ovariectomized groups. After 150 days, both groups were sacrificed to collect the femurs...

ba0003pp120 | Cell biology: osteoblasts and bone formation | ECTS2014

Treatment with allopurinol and oxypurinol promotes osteoblast differentiation and increases bone formation

Orriss Isabel , Arnett Timothy , George Jacob , Witham Miles

Allopurinol and, and its active metabolite, oxypurinol are widely used in the treatment of gout and hyperuricemia. They act by inhibiting xanthine oxidase an enzyme in the purine degradation pathway that converts xanthine to uric acid. This oxygen-dependent reaction also results in the generation of superoxide free radicals. The aim of this investigation was to examine the effect of allopurinol and oxypurinol on osteoblast differentiation and function. Rat calvarial osteoblast...

ba0003pp121 | Cell biology: osteoblasts and bone formation | ECTS2014

Is the bone marrow envelope a reservoir of osteoblast progenitors?

Kristensen Helene Bjoerg , Andersen Thomas Levin , Marcussen Niels , Rolighed Lars , Delaisse Jean-Marie

It is still unknown where from osteoblasts are recruited during remodeling of adult human cancellous bone. Still, it is often suggested that they originate from perivascular osteoprogenitors in the bone marrow (BM). Here we propose also the existence of a layer of osteoprogenitors at the periphery of the BM. This proposal is based on electron and light microscopy of the bone surface and of the neighboring BM in human iliac crest biopsies, where we quantified cell densities, ce...

ba0003pp122 | Cell biology: osteoblasts and bone formation | ECTS2014

Extracellular protein Edil3 stimulates matrix calcification via α5β1 and ERK pathway in MC3T3E1 cells

Oh Sin-Hye , Ryu Je-Hwang , Koh Jeong-Tae

Epidermal growth factor-like repeats and discoidin I-like domains 3 (Edil3) is an extracellular matrix protein that contains Arg-Gly-Asp (RGD) motif to bind to integrins. Recently it has been reported that Edil3 is involved in various biological processes, including angiogenesis and cellular differentiation. This study was to explore the role of Edil3 in osteoblast differentiation and an underlining molecular mechanism. When MC3T3E1 cells were cultured with osteogenic medium, ...

ba0003pp123 | Cell biology: osteoblasts and bone formation | ECTS2014

miR-468 controls Runx2 expression during early differentiation of Runx2 in mice

Cabon Owen , Geoffroy Valerie

We investigated the ability of microRNAs to regulate osteoblast differentiation by controlling distinct aspects of the differentiation process. We have identified by microRNAs microarray a set of miRNAs that are differentially regulated during differentiation of the two osteoblast cell lines MC3T3-E1 and C3H10T1/2. We focused on miR-468 that is a potential regulator of Runx2.In our microarray analysis miR-468 was expressed at a high level and was strongl...

ba0003pp124 | Cell biology: osteoblasts and bone formation | ECTS2014

MiR-320a and miR-483-5p are over-expressed in osteoblasts from osteoporotic fractured hips

Garcia-Giralt Natalia , De-Ugarte Laura , Balcells Susana , Arino-Ballester Sergi , Yoskovitz Guy , Guerri Roberto , Mellibovsky Leonardo , Urreizti Roser , Nogues Xavier , Grinberg Daniel , Diez-Perez Adolfo

MicroRNAs are important regulators of gene expression with documented role in bone metabolism and osteoporosis. Moreover, the use of miRNAs constitutes potential therapeutic targets. Our aim was to identify miRNAs differentially expressed in fractured compared to healthy bone. Additionally, we performed a miRNA profiling of primary osteoblasts to assess the origin of the differentially expressed miRNAs. Total RNA was extracted from fresh femoral neck trabecular bone from women...

ba0003pp125 | Cell biology: osteoblasts and bone formation | ECTS2014

The significance of Bcl-2-associated athanogene-1 (BAG-1)-mediated protein interactions in osteoblast development

Greenhough Joanna , Papadakis Emmanouil , Cutress Ramsey , Townsend Paul , Oreffo Richard , Tare Rahul

BAG-1 interacts with diverse proteins including heat shock proteins (HSC70/ HSP70) and nuclear hormone receptors to modulate cell proliferation, differentiation and apoptosis. We have shown that bone marrow stromal cells (BMSCs) of Bag-1+/− female mice display reduced BMP-2-directed osteogenic differentiation potential. Estradiol binds to estrogen receptors (ERs) and is functionally involved in BMP-directed osteogenic differentiation of osteoprogenito...

ba0003pp126 | Cell biology: osteoblasts and bone formation | ECTS2014

Glucose-dependent insulinotropic polypeptide directly affects collagen deposition and maturation in osteoblast cultures

Mabilleau Guillaume , Mieczkowska Aleksandra , Chappard Daniel

Objectives: A role for glucose-dependent insulinotropic polypeptide (GIP) in controlling extracellular bone matrix deposition and maturation has previously been evidenced in GIP receptor knock-out mice. However, as the GIP receptor is expressed in several tissues other than bone it was difficult to ascertain whether the modifications of extracellular bone matrix were due to direct effects on osteoblasts or indirect through regulation of signals originating from other tissues. ...

ba0003pp127 | Cell biology: osteoblasts and bone formation | ECTS2014

Regulation of adipo- and osteo-genesis of multipotent cells by strontium through stimulation of small Rho GTPases: A 3D bioreactor study

Louis Fiona , Linossier Marie-Therese , Peyroche Sylvie , Vico Laurence , Guignandon Alain

Small GTPases of the Rho family (RhoA and Rac-1) are responsible for cytoskeleton dynamics (particularly actin polymerisation) and control cellular tension. For these reasons, they are implicated in the commitment of multipotent cells (MCs). In one hand, increased tension (important RhoA activity) is commonly associated with osteogenesis (OS), in the other hand, a reduced one (low RhoA activity) is associated with adipogenesis (AD). Nevertheless, precise RhoGTPases regulations...

ba0003pp128 | Cell biology: osteoblasts and bone formation | ECTS2014

Vitamin K2 (MK-7) is involved in bone and energy homeostasis: Effects on osteoblast, adipocyte and β-cell regulatory loops

Gordeladze Jan Oxholm , Reseland Janne Elin , Moller Mona

Uncarboxylated osteocalcin, allegedly via G-protein coupled receptors, interacts with adipocytes and pancreatic β-cells, thus affecting metabolic homeostasis. Here, we demonstrate that MK-7 directly and indirectly, via osteocalcin, modulates the endocrine functions of both β-cells and adipocytes.• It was shown (by applying the Mir@nt@n-based emulation algorithm) that MK-7 was involved in a network of intracellular regulat...

ba0003pp129 | Cell biology: osteoblasts and bone formation | ECTS2014

Modulation of sclerostin expression by estrogen via BMP-2 signaling in human mesenchymal stromal cells and osteoblasts

Kim Ri Youn , Yang Hoon Joo , Song Yun Mi , Cho Tae Hyung , Hwang Soon Jung , Kim In Sook

Little is known about the molecular mechanisms underlying the interaction between Sclerostin (SOST) and estrogen, showing an inverse relation in clinical data. Therefore, we investigated mechanisms by which estrogen modulates Sost expression at human osteoblastic cell level in association with BMP-2 signaling, which is a potential route of SOST induction. BMP-2 significantly induced SOST expression, which is suppressed by 17β-estradiol (E2) in human mesenchymal s...

ba0003pp130 | Cell biology: osteoblasts and bone formation | ECTS2014

Decrease of mineralization by FGF1 is linked to up-regulation of ANKH and OP in osteogenic differentiation and conversion

Simann Meike , LeBlanc Solange , Jakob Franz , Schutze Norbert , Schilling Tatjana

Osteoporosis is generally accompanied by the fatty degeneration of the bone marrow. The enhanced deposition of adipocytes may result from adipogenic differentiation of bone-marrow mesenchymal stem cells (MSC) and from the adipogenic conversion of osteoblastic cells respectively. Thus, whether this clinical observation is a cause of the disease or rather a reaction of the afflicted bone marrow remains to be elucidated.Previous microarray and bioinformatic...

ba0003pp131 | Cell biology: osteoblasts and bone formation | ECTS2014

Hyperbaric oxygen promotes osteogenic differentiation of mesenchymal stem cells by regulating sclerostin expression and Wnt/β-catenin signaling

Lin Song-Shu , Ueng Ueng Steve W.N. , Niu Chi-Chien , Yuan Li-Jen , Yang Chuen-Yung , Chen Wen-Jer , Lee Mel S. , Chen Jan-Kan

Background: Hypoxia induces mesenchymal stem cells (MSCs) proliferation but results in a population with impaired osteogenic differentiation potential. Bone healing of tibial lengthening is enhanced by hyperbaric oxygen therapy. However, little is known about the effects of HBO on the Wnt signaling pathway in MSCs.Materials and methods: MSCs were cultured in complete medium and the osteogenic groups were cultured in osteogenic induction medium. Control c...

ba0003pp132 | Cell biology: osteoblasts and bone formation | ECTS2014

Roles of phospholipase D during physiological and vascular calcification

Abdallah Dina , Hamade Eva , Badran Bassam , Buchet Rene , Mebarek Saida

There is a similarity of the mechanisms of physiological and pathological mineralization. Pathological calcification of soft tissues as the case of vascular calcification is characterized by the deposition of hydroxyapaptite induced by matrix vesicles at least in the initial stage as in the case of physiological calcification in skeletal tissues. Lipid metabolism is involved in the differentiation of smooth muscle cells and bone cells suggesting that phospholipases can modulat...

ba0003pp133 | Cell biology: osteoblasts and bone formation | ECTS2014

Chemical composition of apatites formed by matrix vesicles during bone mineralization

Strzelecka-Kiliszek Agnieszka , Bozycki Lukasz , Buchet Rene , Pikula Slawomir

Bone cells control initial steps of mineralization by forming matrix vesicles (MVs) in which calcium and phosphate ions are accumulated. Under physiological conditions, MVs are released to the extracellular matrix (ECM) and Ca2+ and Pi form hydroxyapatite (HA). Growing minerals break the MVs membrane and are deposited in ECM. Under pathological conditions the mineralization process becomes deregulated, which leads to the formation of calcium phosphate deposits. It is suggested...

ba0003pp134 | Cell biology: osteoblasts and bone formation | ECTS2014

Osteogenic markers are decreased in SHR rats during in vitro osteoblast differentiation

de Barros Thamine Landim , Chaves-Neto Antonio , do Amaral Caril , Brito Victor , Oliveira Sandra

The purpose of this study was evaluated the osteogenic differentiation in vitro of mesenchymal stem cells (MSC) of SHR (Spontaneously Hypertensive Rats) and Wistar (Normotensive) rats.Therefore, MSC from femoral bone marrow of Wistar and SHR rats 4 weeks old were utilized. The experimental protocols were approved by the Animal Experiments Local Ethics Committee (Process 00716-2012). The osteogenic medium (OM) consist of the proliferation medium (MEM) supplemented with...

ba0003pp135 | Cell biology: osteoblasts and bone formation | ECTS2014

High-resolution molecular validation of self-renewal and spontaneous differentiation in adipose-tissue derived human mesenchymal stem cells cultured in human platelet lysate

Dudakovic Amel , Camilleri Emily , Riester Scott , Westendorf Jennifer , Kakar Sanjeev , Dietz Allan , van Wijnen Andre

Improving the effectiveness of adipose-derived human mesenchymal stromal/stem cells (AMSCs) for skeletal therapies requires a detailed molecular characterization of mechanisms supporting cell proliferation and multi-potency of AMSCs. We compared gene expression data obtained by high-throughput RNA sequencing and RT-qPCR of actively proliferating and uninduced post-proliferative AMSCs with flow cytometry data for mesenchymal stem cell markers (e.g., CD44, CD73/NT5E, CD90/THY1 a...

ba0003pp136 | Cell biology: osteoblasts and bone formation | ECTS2014

Transcriptional profile of osteoblastic cells cultured on titanium surfaces modified by oxidative nanopatterning

Ferreira Maidy , Variola Fabio , Nanci Antonio , Dernowsek Janaina , Passos Geraldo , Bombonato-Prado Karina

Titanium implants have been extensively used in dentistry as a replacement of absent dental elements. The biocompatibility of a material depends on cellular response in contact with a surface. The microarray technology is a tool to obtain an overview of the cell state in terms of large-scale transcriptional expression in different situations. Thus, the objective of this investigation was to evaluate the transcriptional profile of osteoblastic cells from human alveolar bone cul...

ba0003pp137 | Cell biology: osteoblasts and bone formation | ECTS2014

IGF1 stimulates protein synthesis by enhancing mRNA translation rate in osteoblasts

Bakker Astrid D. , Gakes Tom , Juffer Petra , Hogervorst Jolanda M.A. , de Wit Gerard M.J. , Klein-Nulend Jenneke , Jaspers Richard T.

IGF1 is an osteo-anabolic factor that stimulates osteogenic precursor cell differentiation. IGF1 is produced in bone in response to mechanical stimulation, but also in mechanically-stimulated muscle cells. IGF1 enhances the rate of mRNA translation in muscle cells via activation of the PI3K/AKT/mTOR pathway, thereby increasing in muscle mass. Therefore we hypothesized that IGF1 not just enhances osteogenic differentiation of precursors, but also stimulates protein syn...

ba0003pp138 | Cell biology: osteoblasts and bone formation | ECTS2014

Metabolism and synthesis activity of calvaria osteoblasts from offspring of rats treated with caffeine during pregnancy

Sena Reis Amanda Maria , Ribeiro Lorena Gabriela Rocha , de Melo Ocarino Natalia , Serakides Rogeria

Caffeine is a methylxanthine found in many foods and is widely consumed by the human population. Therefore, its effects and mechanisms in various tissues have been widely studied. Various bone abnormalities have been observed in fetuses from rats treated with caffeine. But the genesis of these abnormalities is not known. The objective of this study was to verify the metabolism and activity of calvaria osteoblasts from offspring of rats treated with caffeine during pregnancy. T...

ba0003pp139 | Cell biology: osteoblasts and bone formation | ECTS2014

Effect of lactation on the osteogenic potential of bone marrow mesenchymal stem cells of rats

Sena Reis Amanda Maria , de Freitas Silva Juneo , Santos Silvia Silva , Serakides Rogeria , de Melo Ocarino Natalia

The lactation has been considered a cause of bone loss in humans and animals. Studies have shown that during lactation, there is a significant reduction of bone mineral density. Rats at the end of lactation show reduced bone mass. The objective of this study was to verify the effect of lactation under osteogenic potential of bone marrow mesenchymal stem cells (BMMSCs) of female rats. Twelve Wistar rats were distributed among the control and lactating groups. The experiment was...

ba0003pp140 | Cell biology: osteoblasts and bone formation | ECTS2014

Release kinetic of prolyl hydroxylase inhibitors from collagen barrier membranes in vitro

Hamid Omar , Pensch Manuela , Agis Hermann

Collagen barrier membranes are used in guided tissue regeneration to support bone healing and periodontal regeneration. Pharmacological inhibitors of prolyl-hydroxylases (PHD) can support hard and soft tissue regeneration and are therefore a promising tool for periodontal therapy. Here we evaluate the release kinetic of the PHD inhibitors dimethyloxaloylglycine and L-mimosine from the collagen barrier membranes.The PHD inhibitors were lyophilized onto co...

ba0003pp141 | Cell biology: osteoblasts and bone formation | ECTS2014

An infrared assay of the kinetics of phosphate-release from physiological substrates in living cells

Zhongyuan Ren , Leduy Do , Mebarek Saida , Keloglu Nermin , Ahamada Saandia , Meena Saurabh , Magne David , Pikula Slawomir , Wu Yuqing , Buchet Rene

Up to now, most of standard methods for measuring inorganic phosphate (Pi) to determine phosphatase activity are based on coupled enzyme assays, colorimetric methods or conductance measurements. However, none of these methods can measure Pi, substrate and protein concentrations simultaneously, allowing direct kinetic determinations of phosphatase activity in cells in a single assay. Among the enzymes having a pyrophosphatase activity and releasing Pi...

ba0003pp142 | Cell biology: osteoblasts and bone formation | ECTS2014

Cell attachment and proliferation of bone marrow-derived osteoblast on zirconia of various surface treatment

Lee Heesu , Pae Ahran , Noh Kwantae , Woo Yi-Hyung

This study was performed to characterize the effects of calcium phosphate coated and hydroxyapatite coated zirconia compared to smooth surfaced zirconia with bone marrow-derived osteoblast culture.Bone marrow-derived osteoblasts were cultured on i) smooth zirconia, ii) zirconia coated with calcium phosphate (CaP), and iii) zirconia coated with hydroxyapatite (HA). The tetrazolium-based colorimetric assay (MTT test) was used for examining the attachment o...

ba0003pp143 | Cell biology: osteoblasts and bone formation | ECTS2014

Utilization of L-mimosine in pulp regeneration: lessons from cell culture and tooth slice organ cultures

Muller Heinz-Dieter , Trimmel Katharina , Cvikl Barbara , Gruber Reinhard , Agis Hermann

After trauma or carious lesion dental pulp healing is difficult to predict. In addition systemic diseases like diabetes mellitus can impair the regenerative capacity. New regenerative strategies target prolyl hydroxylase (PHD) by pharmacological inhibitors to stimulate hard and soft tissue healing. PHD inhibitors such as L-mimosine (L-MIM) induce vascular endothelial growth factor (VEGF) production by promoting angiogenesis. However, it is unclear if L-MIM is a feasible tool t...

ba0003pp144 | Cell biology: osteoblasts and bone formation | ECTS2014

Pro-osteogenic properties of nacre extract on two cell lines, primary human osteoblasts and MC3T3-E1 cell line

Brion Alice , Zhang Ganggang , Piet Marie-Helene , Moby Vanessa , Bianchi Arnaud , Dossot Manuel , Mainard Didier , Galois Laurent , Gillet Pierre , Rousseau Marthe

Nacre, or mother of pearl, is a promising natural biocompatible biomaterial consisting of aragonite (97%) and of organics (3%) and capable to increase the cell osteogenic activity. It has been established that osteoarthritic osteoblasts present a mineralization defect and, to date, only a few molecules (vitamin D3 and bone morphogenetic protein2) could improve the mineralization potential of this cell type. In this context, we evaluated the impact of nacreous molecules on the ...

ba0003pp145 | Cell biology: osteoblasts and bone formation | ECTS2014

Effects of strontium ranelate on the bone-like mineralized matrix produced in osteoblast cell cultures

Querido William , Campos Andrea , Ferreira Erlon Martins , Gil Rosane San , Rossi Alexandre , Farina Marcos

Strontium ranelate is a promising drug used in the treatment of osteoporosis. This drug has a unique dual effect on bone turnover, simultaneously increasing bone formation by osteoblasts (anabolic effect) and decreasing bone resorption by osteoclasts (antiresorptive effect). The goal of this study was to evaluate: i) anabolic effects of strontium ranelate on the formation of bone-like mineralized matrix in osteoblast cell cultures and ii) changes the drug could cause on the ma...

ba0003pp146 | Cell biology: osteoblasts and bone formation | ECTS2014

Strontium ranelate increases osteoblast cell proliferation and differentiation and the formation of bone-like mineralized matrix on different titanium substrates

Querido William , Farina Marcos , Anselme Karine

The integration of biomaterials into the calcified bone tissue is essential for the clinical success of bone implants. A particular strategy to improve such integration is the use of specific molecules to increase osteoblast cell adhesion, proliferation, and/or differentiation on the surface of the implants, aiming to enhance the interaction of cells with biomaterials. The goal of this study was to better understand the potential of the anti-osteoporotic drug strontium ranelat...

ba0003pp147 | Cell biology: osteoblasts and bone formation | ECTS2014

Differential gene expression of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are modulated during osteogenic/odontogenic differentiation from human dental pulp stem cells (DPSCs) by BMP-7

Paiva Katiucia , Silva Luiz , Sogayar Mari , Labriola Leticia

Constant remodeling of extracellular matrix is a hallmark during physiological conditions, such as stem cell differentiation, embryogenesis and tissue repair. Matrix metalloproteinases (MMP) play a key role in these processes. Mesenchymal stem cells derived from dental pulp are multipotent and have the capacity to differentiate into mesenchymal lineages. Bone morphogenetic proteins (BMP) are a family of signaling molecules critically involved at various stages in the formation...

ba0003pp148 | Cell biology: osteoblasts and bone formation | ECTS2014

Expression of BDNF gene in the femur of aged vs adult rats

Camerino Claudia , Cannone Maria , Heiny Judy , Tricarico Domenico

Brain-derived neurotrophic factor (BDNF) has recently emerged as a factor able to concurrently target age related diseases as neurodegenerative disease, osteoporosis and metabolic syndrome. However the role of BDNF in the frailty syndrome associated with aging has never been investigated. The deletion of central BDNF gene in mice results in increased bone mass and in vitro experiments show that BDNF has a positive effect on bone. BDNF expression is mediated by estroge...

ba0003pp149 | Cell biology: osteoblasts and bone formation | ECTS2014

Telomerase promotes osteoblast differentiation by modulating IGF-signaling pathway

Saeed Hamid , Qiu Weimin , Li Chen , Flyvbjerg Allan , Abdallah Basem , Kassem Moustapha

The molecular mechanisms underlying telomere shortening and telomerase deficiency contributing to defective age-related bone formation are poorly studied. We have previously demonstrated the role of telomerase re-activation in enhancing osteoblast differentiation of BMSCs in vitro and in vivo. Here, we investigated further the signaling pathways underlying the regulatory function of telomerase in osteogenesis. Comparative microarray analysis of telomerase-ove...

ba0003pp150 | Cell biology: osteoblasts and bone formation | ECTS2014

PRKG1: A novel regulator of human skeletal (mesenchymal) stem cell differentiation

Jafari Abbas , Siersbaek Majken , Dobbelstein Matthias , Kassen Moustapha

Protein kinases are an important class of regulatory elements and pharmacological targets for treatment of a number of diseases including cancer, heart and lung diseases. However, their role in regulation of the skeletal (mesenchymal) stem cell (MSC) functions and bone formation is not fully understood. Thus, we performed functional screening of human kinome, using three siRNAs for each of the ~700 known kinases represented in the human genome. Activity of alkaline phosphatase...

ba0003pp151 | Cell biology: osteoblasts and bone formation | ECTS2014

Syndecan-2 is a new negative modulator of Wnt signaling within bone marrow

Mansouri Rafik , Geoffroy Valerie , Marie Pierre , Modrowski Dominique

Wnt factors bind to heparan sulfate chains of syndecans that hence control their distribution in the cell microenvironment and also contribute to the internalization and recycling of the Wnt receptors. Syndecans are therefore likely important Wnt regulators within the bone marrow. Here, we investigated the interactions between Syndecan-2 and Wnt signaling in normal bone cells. We analyzed the effects of 2.3 kb Col1A1 promoter-driven Syndecan-2 overexpression in osteoblasts. Sy...