Bone Abstracts (2015) 4 P61 | DOI: 10.1530/boneabs.4.P61

Association of vitamin D concentrations with 7-Dehydrocholesterol Reductase in school children in a sun-rich, semi-rural setting in Western Maharashtra, India

Rubina Mandlik1, Neha Kajale1, Shashi Chiplonkar1, Vivek Patwardhan1, Veena Ekbote1, Vaman Khadilkar1, Zulf Mughal2 & Anuradha Khadilkar1


1Growth and Endocrine Unit, Hirabai Cowasji Jehangir Medical Research Institute, Jehangir Hospital, Pune, India; 2Royal Manchester Children’s Hospital, Manchester, UK.


Objectives: Studies have reported prevalence of vitamin D deficiency in Indian children with adequate sunlight exposure (Harinarayan, 2008). The objectives of our study were to A) examine the prevalence of vitamin D deficiency in children with adequate sunlight exposure in a semi-rural setting in Western Maharashtra (18(°N), India and B) explore the association of serum 7-Dehydrocholesterol reductase (7-DHCR) with the serum vitamin D levels.

Methods: This cross-sectional study was carried out in 432 children aged 6–12 years from a public school. Data on demographics, anthropometry (height, weight), body composition (BIA (Tanita, BC-420MA)) was collected by standard methods. Sunlight exposure using validated questionnaire and dietary calcium intake (by 3 day, 24 h recall method; analysed by C-diet, V2.0) were recorded. Fasting serum 25 hydroxyvitamin D (25OHD) (EIA kit by DLD Germany) and 7-DHCR on a sub-sample of 160 (EIA kit by Cusabio) were assessed.

Results: Mean serum 25OHD concentrations were 23.4±4.2 ng/ml; 32% children had serum 25 OHD concentrations ≤20 ng/ml (vitamin D inadequacy) and 68% children had levels ≧21 ng/ml (vitamin D sufficiency) (Institute of Medicine, 2011). All children had a similar sunlight exposure of more than 2 h per day (2.4±0.2 h) and similar dietary calcium density (244.5±85.1 mg/1000 kcal per day). The mean DHCR concentrations were 714±341 pg/ml. DHCR was found to be negatively correlated with the 25 OHD concentrations (r=−0.2; P<0.05). A significant negative correlation was also obtained between body fat percentage and serum 25OHD concentrations (r=−0.2; P<0.01).

Conclusion: Deficiency of vitamin D was noted in a third of the children despite adequate sunlight exposure. higher DHCR concentrations and body fat percentage were associated with lower concentrations of serum vitamin D. Data collection is still in progress.

Disclosure: The authors declared no competing interests.

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