Bone Abstracts (2015) 4 P85 | DOI: 10.1530/boneabs.4.P85

Comparison of three detection assays of serum 25(OH) D

Shanshan Wu, Huifeng Zhang & Xiao Han


The Second Hospital of Hebei Medicine University, Shijiazhuang, China.


The skeletal effects and new biological effect of Vitamin D are receiving increased attention. However detection methods in 25-hydroxyvitamin D (25(OH) D) measurement are not standardized and variation between laboratories ahd methods has become attractive. This study emphasizes relativity and consistency between ELISA, ESI-LC-MS/MS and electrochemical luminescence analysis which are used widely in China. This study collected 64 cases of blood, and finally obtained good relativity and consistency in every two assays, especially between ELISA and ESI-LC-MS/MS, through linear regression analysis and Bland-Altman analysis. Chinese laboratories can choose any one from the three assays according to its own condition. This study provides the reference for vitamin D deficiency, excess assessment and monitoring as well as optimal level exploration in China.

Disclosure: The authors declared no competing interests.

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