ECTS2016 Poster Presentations Osteoporosis: evaluation and imaging (39 abstracts)
Inducing osteoporosis in a large animal model: influence of ovariectomy, diet and corticoid therapy
Jonas Sender 1, , Thaqif El Khassawna 1 , Georg Erdmann 1 , Wolfgang Böcker 4, , Gabriele A. Krombach 1, , Alexander C. Langheinrich 1, & Marian Kampschulte 1,
1Laboratory for Experimental Radiology, Justus-Liebig University, Gießen, Germany; 2Department of Radiology, University Hospital of Giessen, Gießen, Germany; 3BG Trauma Hospital, Frankfurt, Germany; 4Department of Trauma Surgery Giessen, University Hospital of Giessen-Marburg, Gießen, Germany; 5Laboratory for Experimental Trauma Surgery, Gießen, Germany.
Purpose: The aim of our study is a micro-CT-based, longitudinal characterisation of ovariectomy, multideficient diet and corticoid therapy impact on the ossal microarchitecture.
Materials and methods: Four randomized groups were formed out of 32 adult female merino land sheep:
1. control
2. ovariectomized (OVX)
3. OVX+multideficient diet (OVX-D)
4. OVX-D+320 mg methylprednisolonacetat every 14 days (OVX-D-S)
Biopsies of the iliac crest were taken initially, after 3 and after 8 months. Micro-CT-based examinations of the biopsies using an isotropic voxel size of 7 μm regarding bone mineral density (BMD), relative bone volume (BV/TV), trabecular thickness (Tb.Th.), trabecular number (Tb.N.), trabecular separation (Tb.Sp.) and structural model index (SMI) were taken.
Results: The data showed no sign of osteoporosis in the control-group, in the OVX- and OVX-D group.
However, a significant change in bone mineralisation and bone architecture in the OVX-D-S group could be shown after 3 and 8 months. BMD (231.4±45.9 mg/cm3 vs 167.59±25.8 mg/cm3 vs 135.7±26.7 mg/cm3; P<0.05), BV/TV (21.2±3.8% vs 13.7±1.8% vs 12.9±2.1%; P<0.05), Tb.Th. (143±13 μm vs 101±11 μm vs 88±6 μm; P<0.05) and Tb.Sp. (581±82 μm vs 631±82 μm vs 627±91 μm; P<0.05).
With regard to Tb.N. and SMI no significant changes in the OVX-D-S group could be shown.
Conclusion: When comparing the different groups, significant changes could be found regarding BMD and microarchitecture by combining OVX, diet and methylprednisolone. This may be an interesting model for preclinical osteoporosis research, especially with regard to the development of bone substitute materials.
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