Searchable abstracts of presentations at key conferences on calcified tissues
Bone Abstracts (2016) 5 LB1 | DOI: 10.1530/boneabs.5.LB1

ECTS2016 Late Breaking Abstracts (1) (18 abstracts)

All-trans retinoic acid can antagonize osteoblastogenesis induced by different BMPs irrespective of their dimerization types and dose- efficiencies

Yi Liu 1 , Jing Guo 2 , Zhen Lin 3 , Daniel Wismeijer 1 , Haiping Lu 2 & Gang Wu 1


1Department of Oral Implantology and Prosthetic Dentistry, Academic Centre of Dentistry Amsterdam (ACTA), VU University and University of Amsterdam, MOVE Research Institute, 1081LA Amsterdam, The Netherlands; 2School of Stomatology, Zhejiang Chinese Medical University, Hangzhou 310053, China; 3Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou North Avenue 1838, Guangzhou 510515, Guangdong Province, P.R. China.


Introduction: Alcoholism can result in a compromised regenerative capacity of bone and delayed osteointegration of dental implants. One of the mechanisms is that the thereby overdosed all-trans retinoic acid (ATRA), a main metabolite of alcohol, can significantly inhibit osteoblastogenesis. Bone morphogenetic proteins, potent osteinductive growth factors, can be applied to promote osteogenesis. We previously showed that heterodimerized BMP2/7 could promote osteoblastogenesis in a significantly higher dose-efficiency than homodimerized BMP2 or BMP7. In this study, we wish to uncover the antagonism of ATRA to the BMPs of different dimerization types and dose-efficiencies.

Materials and methods: We assessed the antagonism of 1 μM ATRA to either heterodimerized BMP2/7 or homodimerized BMP2 or BMP7 at 50 ng/ml in a well-established eosteoblastogenesis model with pre-osteoblasts (MC3T3-E1). We measured the following parameters: metabolic activity, alkaline phosphatase activity (early differentiation), osteocalcin (late differentiation), mineralization (final differentiation) and the expression of osteoblastogenesis-related genes.

Results: All the three BMPs could significantly enhance ALP, osteocalcin expression and osteoblastogenesis-related genes and BMP2/7 exhibited a significantly higher efficiency than BMP2 or BMP7. ATRA could significantly antagonize such effects of both heterodimerized BMP2/7 and homodimerized BMP2 or BMP7. On the 28th day, BMP2/7, BMP2 and BMP7 resulted in 3-, 1.79- and 1.24-fold mineralization respectively compared with the control (no BMP, no ATRA). However, the addition of ATRA led to a significantly decreased mineralization to a similar level as the control irrespective of BMPs’ dimerization types and potencies.

Conclusions: The advantageous osteoinductivity of heterodimerized BMP2/7 over the homodimerized ones was significantly compromised by ATRA.

Volume 5

43rd Annual European Calcified Tissue Society Congress

Rome, Italy
14 May 2016 - 17 May 2016

European Calcified Tissue Society 

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