ECTS2016 Poster Presentations Bone development/growth and fracture repair (35 abstracts)
In vitro investigation of the effect of Magnetic Resonance guided Focused Ultrasound Surgery on osteosarcoma cell lines
Valentina Agnese 1 , Viviana Costa 1 , Valeria Carina 1 , Daniele Bellavia 1 , Lavinia Raimondi 1 , Angela De Luca 1 , Gianluca Scoarughi 3 , Massimo Midiri 3 , Giorgio Stassi 3 & Gianluca Giavaresi 1,
1Istituto Ortopedico Rizzoli, Palermo, Italy; 2Istituto Ortopedico Rizzoli, Bologna, Italy; 3Università di Palermo, Palermo, Italy.
Aim: Magnetic Resonance guided Focused Ultrasound Surgery (MRgFUS) is an invasive treatment able to control local disease and pain of bone tumors. Unfortunately, there is not any scientific evidence of the biological effect of MRgFUS treatment on tumor cells, especially in lower dose region, where tissues are only warmed to sub-lethal temperatures. Here we investigate the effect of in vitro MRgFUS treatment, at different levels of acoustic energy (200–630 J), on cell viability and osteogenic differentiation (ALPL, RUNX2, SPP1 and BGLAP genes expression) of osteosarcoma cell lines.
Material and methods: The Mg-63 and Saos-2 cell lines were exposed to the MRgFUS treatment (ExAblate 2000 system, In Sightec Ltd., Haifa, Israel) and analyzed for their viability and osteogenic differentiation at three different experimental times: 24 hours, 7 days and 14 days. The proliferation of osteosarcoma cell lines was evaluated by a CellTiter-Glo Luminescence Assay (Promega Fitchburg, WI, USA) whereas the relative gene expression was quantified through the comparative Ct method (StepOne Real-Time PCR System, Applied Biosystems).
Results: Cell viability assay of Mg-63 cell line exposed to MRgFUS showed a higher cell viability 14 days after treatment. On the contrary, the treatment of Saos-2 cell lines with focused ultrasound waves at low intensity (200–400 J) does not cause significant cell viability variation, comparing to the control group. These data were confirmed by the analysis of gene expression which, albeit with variations among the different experimental conditions, they do not seem to highlight a progression to cell’s differentiation.
Conclusion: These data suggest that MRgFUS treatment at low intensity may induce cellular proliferation in vitro. Further analysis are necessary in order to better define, in the clinical practice, the area of ablative treatment and reduce the possibility of inducing cancer cell’s proliferation.
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Bone Abstracts
ISSN 2052-1219 (online)
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