Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp203 | Cell biology: osteoblasts and bone formation | ECTS2013

Connectivity Map-based discovery of novel compounds that induce osteoblast differentiation

Brum A M , van de Peppel J , van Kerkwijk A , Janssen M , Schreuders-Koedam M , Strini T , Eijken M , van Leeuwen J P T M , van der Eerden B C J

Osteoporosis is a common skeletal disorder characterized by low bone mass leading to increased bone fragility and fracture susceptibility. Little is currently known about what specific factors stimulate osteoblast differentiation from human mesenchymal stem cells (hMSCs). Therefore, the aim for this project is to determine novel factors and mechanisms involved in human bone production which can be targeted to treat osteoporosis, using gene expression profiling and bioinformati...

ba0002oc11 | Biology | ICCBH2013

Connectivity map-based discovery of novel compounds that induce osteoblast differentiation

Brum A M , de Peppel J van , van Kerkwijk A , Janssen M , Schreuders-Koedam M , Strini T , Eijken M , van Leeuwen J P T M , van der Eerden B C J

Osteoporosis is a common skeletal disorder characterized by low bone mass leading to increased bone fragility and fracture susceptibility. Little is currently known about what specific factors stimulate osteoblast differentiation from human mesenchymal stem cells (hMSCs). Therefore, the aim for this project is to determine novel factors and mechanisms involved in human bone production which can be targeted to treat osteoporosis, using gene expression profiling and bioinformati...

ba0005p5 | Arthritis and other joint diseases: translational and clinical | ECTS2016

The effects of hydroxychloroquine on bone turnover

Both T , van der Eerden B C J , Koedam M , Zillikens M C , van Laar J A M , Dalm V A S H , van Leeuwen H P T M , van Hagen P M , van Daele P L A

Introduction: We recently showed that patients with primary Sjögren Syndrome (pSS) have significantly higher bone mineral density (BMD) in the lumbar spine and femoral neck compared with healthy controls. The majority of those patients (69%) were using hydroxychloroquine (HCQ), which may have favourable effects on BMD.Aim: To evaluate whether HCQ modulates human bone cells in vitro.Methods: Osteoblasts were differenti...

ba0005p61 | Bone development/growth and fracture repair | ECTS2016

Advanced phase gestational jet lag reduces bone mass of adult offspring

van der Eerden Bram , Chaves Ines , Azimzadeh Pedram , Schreuders-Koedam Marijke , Reiss Irwin , van Leeuwen Johannes , van der Horst Gijsbertus

The mammalian circadian clock is tightly controlled by clock genes, which have been shown to regulate up to 20% of the transcriptome in any given tissue. Evidence is accumulating that light-modulation perpetually affects circadian clock performance. In accordance, shift work or chronic jet lag is associated with higher risk of disease later in life, including osteoporosis. In this study, we assessed whether gestational jet lag in mice reduces bone mass postnatally.<p class...

ba0002p44 | (1) | ICCBH2013

A homozygous mutation in the DNA binding domain of human vitamin D receptor causes vitamin D resistant rickets

van der Eerden Bram , van der Heyden Josine , Hamburger Jan Piet , Schreuders-Koedam Marijke , Asmawidjaja Patrick , de Muinck Keizer-Schrama Sabine , Lubberts Erik , van Leeuwen Johannes , Drop Stenvert

In this case report, we present a brother and sister with hereditary vitamin D resistant rickets (HVDRR). Both children presented at the age of 18 months with severe rickets and elevated serum levels of 1,25-(OH)2D3. They differ from each other in that the girl presented with hypophosphatemia instead of hypocalcemia. Besides, she developed alopecia earlier than the boy and needed more 1,25-(OH)2D3 supplementation. Interestingly, the ...

ba0004is20biog | (1) (1) | ICCBH2015

Bone morbidity in children with leukemia

Ward Leanne M

Biographical DetailsDr Leanne M Ward is an Associate Professor of Pediatrics at the University of Ottawa where she holds a Research Chair in Pediatric Bone Health. She is the Medical Director of the Pediatric Bone Health Clinical and Research Programs at the ChildrenÂ’s Hospital of Eastern Ontario (CHEO) and a pediatric endocrinologist within the Division of Endocrinology and Metaboli...

ba0004is24biog | (1) (1) | ICCBH2015

The new histology

Misof Barbara M

Biographical DetailsBarbara M Misof is Staff Scientist at the Ludwig Boltzmann Institute of Osteology, Vienna, Austria. She completed her PhD in Physics and Postgraduate Education in Medical Physics at the University of Vienna in 2000. In 2002, she received the Herbert-Czitober-Research Award of the Austrian Society of Bone and Mineral Research. Her areas of interest are bone material pro...

ba0001pp237 | Cell biology: osteocytes | ECTS2013

Nucleotide and mechanically induced ATP release pathways in osteocytes

Kringelbach Tina M , Novak Ivana , Schwarz Peter , Jorgensen Niklas Rye

Background: We have previously shown that MLO-Y4 osteocytes express a number of P2 receptors, respond to a broad range of nucleotides (e.g. UTP) by increasing intracellular calcium concentration and release ATP upon both mechanical and UTP stimulation. The aim of this study therefore is to investigate how the osteocytes release ATP and whether there is a difference in release pathway depending on the type of stimulus.Methods: ATP release was investigated...

ba0005p237 | Genetics and Epigenetics | ECTS2016

Association among oxidative stress, Wnt signaling and trabecular bone microstructure in osteoporosis and osteoarthritis

Giner Merce , Miranda Cristina , Jose Montoya M. , Portal Sergio , Angeles Vazquez M. , Jose Miranda M. , Esbrit Pedro , Perez-Cano Ramon

Experimental studies suggested that both, oxidative stress and the Wnt pathway, are important factors in the regulation of bone remodeling. Thus, low antioxidant levels and elevated markers of Wnt pathway inhibitors (sclerostin) levels are associated with a reduced bone mineral density and increased risk of osteoporotic fracture. Whether oxidative stress and the Wnt pathway are related to fracture risk is poorly understood.M&M: Cross-sectional study ...

ba0005p109 | Cancer and bone: basic, translational and clinical | ECTS2016

Antiproliferative properties of oleuropein in human osteosarcoma cells

Moran Jose M , Leal-Hernandez Olga , Canal-Macias Maria L , Lavado-Garcia Jesus M , Roncero-Martin Raul , Aliaga Ignacio , Pedrera-Zamorano Juan D

Background: Cancer is one of the leading causes of death worldwide. Natural products have been regarded as important sources of potential chemotherapeutic agents. In this study, we evaluated the antiproliferative activity of oleuropein, an olive oil compound traditionally found in the Mediterranean diet.Design and Methods: The antiproliferative activity on two human osteosarcoma cell lines (MG-63 and Saos2) was evaluated in vitro using the MTT c...