Searchable abstracts of presentations at key conferences on calcified tissues

ba0005cabs.op2.1 | Oral Poster Talks | ECTS2016

Antagonizing Mir-218 prevents breast cancer-induced osteolytic disease

Taipaleenmaki Hanna , van Wijnen Andre J , Stein Janet L , Hesse Eric , Stein Gary S , Lian Jane B

Signaling pathways crucial in bone development, including Wnt, are also upregulated in breast cancer cells to promote tumor growth in the skeleton, a process known as osteomimicry. Thus, we hypothesized that bone metastatic tumor cells also aberrantly express osteogenic miRNAs to support osteomimetic properties. We have previously shown that miR-218 is highly expressed in osteoblasts and promotes osteogenic differentiation. Interestingly, expression analysis revealed a signifi...

ba0002p152 | (1) | ICCBH2013

Zinc supplementation improves bone density in young adults with thalassemia

Fung Ellen B , Kwiatkowski Janet L , Huang James N , Gildengorin Ginny , King Janet C , Queisser Anne C , Vichinsky Elliott P

Poor bone mineralization remains a major health problem in patients with Thal and has been linked to functional zinc deficiency despite adequate dietary intake. The global etiology of poor bone mineralization includes inadequate dietary intake of calcium and vitamin D, endocrinopathies leading to disturbed calcium homeostasis, dysregulation of the GH–IGF1 axis, and delayed puberty, all resulting in limited growth, decreased bone formation and increased bone resorption. Th...

ba0003pp143 | Cell biology: osteoblasts and bone formation | ECTS2014

Utilization of L-mimosine in pulp regeneration: lessons from cell culture and tooth slice organ cultures

Muller Heinz-Dieter , Trimmel Katharina , Cvikl Barbara , Gruber Reinhard , Agis Hermann

After trauma or carious lesion dental pulp healing is difficult to predict. In addition systemic diseases like diabetes mellitus can impair the regenerative capacity. New regenerative strategies target prolyl hydroxylase (PHD) by pharmacological inhibitors to stimulate hard and soft tissue healing. PHD inhibitors such as L-mimosine (L-MIM) induce vascular endothelial growth factor (VEGF) production by promoting angiogenesis. However, it is unclear if L-MIM is a feasible tool t...

ba0001pp32 | Bone biomechanics and quality | ECTS2013

Vitamin D levels of >85 nmol/l in the presence of adequate dietary Ca minimise bone turnover and improve bone strength

O'Loughlin Peter , Lee Alice , Anderson Paul , Steck Roland , Forwood Mark , Sawyer Rebecca , Morris Howard

We have reported femoral osteopenia in short term-vitamin D restricted rats without deterioration in tibial cortical bone volume (CBV), geometry or strength1. This study aimed to establish the effect of extended vitamin D deficiency in aged rat tibial volume and strength. Female Sprague-Dawley rats (9 m, n=6/group) were fed a diet containing varying vitamin D3 (D) levels (0, 2, 12, and 20 IU/day) with either low (0.1%, LCa) or high (1%, HCa) diet...

ba0005p155 | Cell biology: osteoblasts and bone formation | ECTS2016

L-Carnitine facilitates mitochondrial activity and osteogenic differentiation in human primary osteoblast culture

Vacante Fernanda , Montesano Anna , Spinello Alice , Bolamperti Simona , Senesi Pamela , Terruzzi Ileana , Luzi Livio , Villa Isabella , Rubinacci Alessandro

Osteoblasts have a high rate of energy consumption during bone formation and bone protein synthesis. Therefore, it is likely that decreased energy production that accompanies aging, could contribute to reduced osteoblast activity, a critical feature of senile osteoporosis, and that this reduction might be counteracted by favoring energy availability. Cells of the osteoblastic lineage generate 40–80% of the requested energy through fatty acid degradation, thus the modulati...

ba0002oc26 | Chronic diseases | ICCBH2013

Vertebral fractures in the 3-year period following steroid initiation among children with chronic illnesses

Miettunen P M , Taljaard M , Alos N , Atkinson S , Cabral D , Clarson C , Couch R , Cummings E A , Feber J , Grant R M , Lentle B , Matzinger M , Nadel H , Rodd C , Shenouda N , Stein R , Stephure D , Taback S , Rauch F , Siminoski K , Ward L M , the Canadian STOPP Consortium

Objectives: To describe the incidence of vertebral fractures in steroid-treated children.Methods: Fractures were assessed prospectively each year for 3 years according to the Genant semi-quantitative method. Proportions of children with incident fractures were determined annually over the study period. To examine associations with baseline clinical factors, the 3-year total number of incident fractures was analyzed using multivariable Poisson regression....

ba0004is14biog | (1) (1) | ICCBH2015

Somatic mosaic skeletal overgrowth disorders

Warman Matthew L

Biographical DetailsDr Matthew L Warman is the Harriet M Peabody Professor of Orthopaedic Surgery and Genetics at Harvard Medical School. He attended college at Brown University and Medical School at Cornell University. While in medical school, he performed research with Dr Adele Boskey at The Hospital for Special Surgery. After medical school he trained in Pediatrics at the Children&#146...

ba0003pp223 | Osteoporosis: evaluation and imaging | ECTS2014

Bone mineral density and bone turnover markers in patients with thyroid cancer and L-T4 suppressive therapy after 25 years of follow-up

de Mingo Dominguez Maria Luisa , Iglesias Sonsoles Guadalix , Alvarez Maria Begona Lopez , Diaz-Guerra Guillermo Martinez , Carranza Federico Hawkins

Background: Patients with differentiated thyroid cancer (DTC) are treated with L-thyroxine (L-T4) in relatively hight doses to suppress endogenous TSH levels. Very long-term effect of thyroid hormones supplementation on bone are controversial.Objetive: To study the possible negative effects on bone mineral density (BMD) and bone markers in DTC patients followed in our center.Methods: A 46 po...

ba0004is14 | (1) (1) | ICCBH2015

Somatic mosaic skeletal overgrowth disorders

Warman Matthew L

I will describe non-cancerous skeletal diseases that occur as a consequence of somatic mutation. I will introduce the work of Drs Mary Lyon, Dorothea Bennett, and Rudolf Happle that provided insights into the mechanism responsible for several genetic, non-heritable diseases. I will then describe the technology and analytic strategies that several laboratories, including my own, employed to identify mutations in patients with Maffucci, Proteus, CLOVES, and Klippel-Trenaunay syn...

ba0002p36 | (1) | ICCBH2013

Vitamin D deficiency and its relationship to parathyroid hormone in morbidly obese adolescents prior to bariatric surgery

Censani Marisa , Stein Emily , Shane Elizabeth , Oberfield Sharon , McMahon Donald , Lerner Shulamit , Fennoy Ilene

Objectives: Obese adults commonly have vitamin D deficiency and secondary hyperparathyroidism prior to bariatric surgery. Whether similar metabolic abnormalities exist in morbidly obese adolescents is not known. This study investigated the prevalence of vitamin D deficiency and evaluated the relationship between vitamin D and parathyroid hormone (PTH) in morbidly obese adolescents undergoing evaluation for bariatric surgery.Methods: A cross-sectional stu...