Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp141 | Cell biology: osteoblasts and bone formation | ECTS2014

An infrared assay of the kinetics of phosphate-release from physiological substrates in living cells

Zhongyuan Ren , Leduy Do , Mebarek Saida , Keloglu Nermin , Ahamada Saandia , Meena Saurabh , Magne David , Pikula Slawomir , Wu Yuqing , Buchet Rene

Up to now, most of standard methods for measuring inorganic phosphate (Pi) to determine phosphatase activity are based on coupled enzyme assays, colorimetric methods or conductance measurements. However, none of these methods can measure Pi, substrate and protein concentrations simultaneously, allowing direct kinetic determinations of phosphatase activity in cells in a single assay. Among the enzymes having a pyrophosphatase activity and releasing Pi...