Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp37 | Bone biomechanics and quality | ECTS2014

Bone matrix mineralization after sclerostin antibody treatment in a mouse model of osteogenesis imperfecta

Roschger Andreas , Roschger Paul , Kneissel Michaela , Rauch Frank

Children with osteogenesis imperfecta (OI) still suffer from frequent fractures, despite bisphosphonate treatment. Thus new therapeutic approaches are needed. Sclerostin is a protein that is thought to inhibit bone formation. Treatment with sclerostin antibodies (SclAB) increases bone mass in animal models and in clinical trials and may be a rational therapy for OI as well.Transgenic (TgOI) Col1a1Jrt/+ mice were gene...

ba0003pp174 | Cell biology: osteocytes | ECTS2014

Structural analysis of tooth and jawbone in a type 2 diabetes mouse model

Repp Felix , Kollmannsberger Philip , Roschger Andreas , Roschger Paul , Wagermaier Wolfgang , Fratzl Peter , Weinkamer Richard

In type 2 diabetes mellitus (T2DM) patients, an increased fracture risk is observed, although the bone mineral density is even higher than in non-diabetic patients. This raises the question of the quality of the organic and inorganic matrix in bone1–3. T2DM is also known to forward dysfunctions in the development of soft tissues such as brittle skin due to cross-linking of the collagen or inflammation of the gingiva. For the latter, a possible influence of diab...

ba0005p37 | Bone biomechanics and quality | ECTS2016

Elemental composition of compact human bone correlated with the osteocyte network

Roschger Andreas , Roschger Paul , Repp Felix , Wagermaier Wolfgang , Weinkamer Richard , Klaushofer Klaus , Fratzl Peter

Spatial distributions of major and minor chemical elements are supposed to change during tissue maturation and due to bone diseases. Previous studies suggested that osteocytes have the ability to interact with the bone matrix of their environment. For this interaction osteocytes make use of the big inner surface of the osteocyte lacuno-canalicular network (OLCN) in which they are accommodated. The aim of this study was to quantify spatial correlations between the elemental com...

ba0004oc10 | (1) | ICCBH2015

Lack of PEDF within the bone matrix is associated with osteoidosis and abnormally high bone mineral content

Fratzl-Zelman Nadja , Schmidt Ingo , Roschger Paul , Roschger Andreas , Reich Adi , Glorieux Francis H. , Wagermaier Wolfgang , Fratzl Peter , Klaushofer Klaus , Marini Joan C. , Rauch Frank

Background and methods: Pigment epithelium-derived factor (PEDF) is a potent antiangiogenic factor, ubiquitously expressed and secreted in human tissues. Hypertrophic cartilage and osteoblasts express PEDF that binds to type I collagen and glycosaminoglycans in the extracellular matrix. Two rare forms of osteogenesis imperfecta (OI) with intact collagen synthesis are associated with PEDF deficiency. Histological observations revealed excessive osteoid formation and prolonged m...

ba0005p114 | Cancer and bone: basic, translational and clinical | ECTS2016

Increased zinc accumulation in mineralized osteosarcoma tissue

Rauwolf Mirjam , Pemmer Bernhard , Roschger Andreas , Turyanskaya Anna , Simon Rolf , Lang S. , Puchner S E , Windhager Reinhard , Klaushofer Klaus , Wobrauschek Peter , Hofstaetter Jochen G , Roschger Paul , Streli Christina

Abnormal tissue levels of certain trace elements such as Zinc (Zn) were reported in various types of cancer. Little is known about the role of Zn in osteosarcoma.Using confocal synchrotron radiation micro X-ray fluorescence analysis, we characterized the spatial distribution of Zn in high-grade (G3) sclerosing osteosarcoma of nine patients (4 f/5 m; 7 knee/1 humerus/1 femur) following chemotherapy and wide surgical resection. The study was done in accord...

ba0005p312 | Osteoporosis: evaluation and imaging | ECTS2016

Manganese distribution in bone tissue by SR-μXRF

Turyanskaya Anna , Rauwolf Mirjam , Roschger Andreas , Prost Josef , Pemmer Bernhard , Simon Rolf , Roschger Paul , Hofstaetter Jochen G. , Landete-Castillejos Tomas , Wobrauschek Peter , Streli Christina

Contemporary elemental imaging techniques are greatly contributing into the bone research. Synchrotron radiation induced confocal micro x-ray fluorescence technique (SR-μXRF) was employed for the analysis, being the most powerful and sufficient tool in detection and characterization of trace element distributions in bone tissue. Manganese (Mn), as a potential contributor into the mechanisms of calcium incorporation into bone tissue is in the spotlight of research. Further...