Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp132 | Cell biology: osteoblasts and bone formation | ECTS2014

Roles of phospholipase D during physiological and vascular calcification

Abdallah Dina , Hamade Eva , Badran Bassam , Buchet Rene , Mebarek Saida

There is a similarity of the mechanisms of physiological and pathological mineralization. Pathological calcification of soft tissues as the case of vascular calcification is characterized by the deposition of hydroxyapaptite induced by matrix vesicles at least in the initial stage as in the case of physiological calcification in skeletal tissues. Lipid metabolism is involved in the differentiation of smooth muscle cells and bone cells suggesting that phospholipases can modulat...

ba0003pp133 | Cell biology: osteoblasts and bone formation | ECTS2014

Chemical composition of apatites formed by matrix vesicles during bone mineralization

Strzelecka-Kiliszek Agnieszka , Bozycki Lukasz , Buchet Rene , Pikula Slawomir

Bone cells control initial steps of mineralization by forming matrix vesicles (MVs) in which calcium and phosphate ions are accumulated. Under physiological conditions, MVs are released to the extracellular matrix (ECM) and Ca2+ and Pi form hydroxyapatite (HA). Growing minerals break the MVs membrane and are deposited in ECM. Under pathological conditions the mineralization process becomes deregulated, which leads to the formation of calcium phosphate deposits. It is suggested...

ba0003pp141 | Cell biology: osteoblasts and bone formation | ECTS2014

An infrared assay of the kinetics of phosphate-release from physiological substrates in living cells

Zhongyuan Ren , Leduy Do , Mebarek Saida , Keloglu Nermin , Ahamada Saandia , Meena Saurabh , Magne David , Pikula Slawomir , Wu Yuqing , Buchet Rene

Up to now, most of standard methods for measuring inorganic phosphate (Pi) to determine phosphatase activity are based on coupled enzyme assays, colorimetric methods or conductance measurements. However, none of these methods can measure Pi, substrate and protein concentrations simultaneously, allowing direct kinetic determinations of phosphatase activity in cells in a single assay. Among the enzymes having a pyrophosphatase activity and releasing Pi...

ba0007lb5 | (1) | ICCBH2019

Chronic recurrent multifocal osteomyelitis in children with hypophosphatasia explained by anti-inflammatory nucleotidase activity of tissue nonspecific alkaline phosphatase in mesenchymal and hematopoietic cells

Bessueille Laurence , Briolay Anne , Como Juna , Mansouri Cylia , Gleizes Marie , El Jamal Alaeddine , Buchet Rene , Dumontet Charles , Matera Eva-Laure , Mornet Etienne , Millan Jose Luis , Fonta Caroline , Magne David

Deficiency in tissue nonspecific alkaline phosphatase (TNAP) causes hypophosphatasia (HPP), which is mainly characterized by skeletal hypomineralization. TNAP promotes mineralization by dephosphorylating the mineralization inhibitor inorganic pyrophosphate (PPi), which is generated from adenosine triphosphate (ATP) by ectonucleotide pyrophosphatase phosphodiesterase 1 (NPP1). Chronic recurrent multifocal osteomyelitis (CRMO), a sterile bone auto-inflammatory disease, has been ...

ba0007lb5 | (1) | IMPE2023

Chronic recurrent multifocal osteomyelitis in children with hypophosphatasia explained by anti-inflammatory nucleotidase activity of tissue nonspecific alkaline phosphatase in mesenchymal and hematopoietic cells

Bessueille Laurence , Briolay Anne , Como Juna , Mansouri Cylia , Gleizes Marie , El Jamal Alaeddine , Buchet Rene , Dumontet Charles , Matera Eva-Laure , Mornet Etienne , Millan Jose Luis , Fonta Caroline , Magne David

Deficiency in tissue nonspecific alkaline phosphatase (TNAP) causes hypophosphatasia (HPP), which is mainly characterized by skeletal hypomineralization. TNAP promotes mineralization by dephosphorylating the mineralization inhibitor inorganic pyrophosphate (PPi), which is generated from adenosine triphosphate (ATP) by ectonucleotide pyrophosphatase phosphodiesterase 1 (NPP1). Chronic recurrent multifocal osteomyelitis (CRMO), a sterile bone auto-inflammatory disease, has been ...