Searchable abstracts of presentations at key conferences on calcified tissues

ba0001oc6.4 | Mineralisation and energy metabolism | ECTS2013

Inhibition of PTH-induced vasorelaxation modulates its anabolic action

Gohin Stephanie , Chenu Chantal , Pitsillides Andrew , Arnett Timothy , Marenzana Massimo

The relationship between bone formation and blood flow is unclear. Recently, PTH was reported to activate production of nitric oxide (NO), a potent vasorelaxing agent, in endothelial cells and we and others have confirmed a strong vasorelaxing action of PTH in vivo in the mouse. Here, we tested the hypothesis that a potent NO synthase inhibitor (L-NAME: NG-nitro-L-arginine methyl ester) may alter the effect of intermittent PTH (iPTH) on b...

ba0005oc4.5 | Catabolism and metabolism | ECTS2016

Effects of glucagon-like peptide-1 receptor agonists on bone blow flow and architecture in diabetic mice

Pereira Marie , Gohin Stephanie , Cleasby Mark , Chenu Chantal

Type 2 diabetes mellitus prevalence increases with age and the disease predisposes to increased risk of fractures and skeletal complications. One possible contributor to skeletal weakening in diabetes is a decline in blood supply. We hypothesised that bone blood flow is impaired in diabetic mice and that chronic administration of glucagon-like peptide-1 receptor agonists (GLP-1RA) can increase blood flow to bone, thereby stimulating bone formation and improving bone architectu...

ba0001oc2.6 | Bone quality and fracture repair - animal models | ECTS2013

GH excess in bGH transgenic mice adversely affects bone density, architecture and quality

Lim Su-Vern , Marenzana Massimo , List Edward , Kopchick John , Korbonits Marta , Chenu Chantal

GH is an important anabolic hormone involved in the regulation of longitudinal bone growth. However, acromegaly patients have a higher prevalence of vertebral fractures despite normal bone mineral density (BMD), suggesting that GH overexpression has adverse effects on skeletal architecture and strength. We used giant bovine GH (bGH) transgenic mice to analyse the effects of high serum GH levels on BMD, architecture and mechanical strength. Five month-old hemizygous male bGH mi...

ba0005p160 | Cell biology: osteoblasts and bone formation | ECTS2016

Effect of melatonin on in vitro bone remodelling and blood supply

Gohin Stephanie , Pereira Marie , Belgrave Naomi Weekes , Lee Ji Sun , Tompkins Hayley , Orriss Isabel , Chenu Chantal

Melatonin is a neuro-hormone released primarily from the pineal gland, which has been shown to have bone anabolic effect, although it is still unclear whether its skeletal action is directly mediated by receptors expressed on bone cells or is indirect. In this study, we examined melatonin’s effects on bone cellular activities in vitro and tested whether it modifies angiogenesis and blood flow to bone, which are both essential for bone formation.<p class="abst...