Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp272 | Genetics | ECTS2013

Comparison of gene expression in osteoblasts from patients of Polynesian and Caucasian ethnicities

Naot Dorit , Bava Usha , Choi Ally , Callon Karen , Pitto Rocco , Bentley Jarome , Gamble Greg , Cornish Jillian

Polynesians have higher bone mineral density and lower rate of hip fracture compared to age-matched Caucasian in New Zealand, and anecdotal evidence suggests that bones of Polynesian patients heal much faster than those of Caucasians. We compared gene expression in osteoblasts cultured form bone samples taken from patients of Polynesian and Caucasian origin, in order to identify genes and pathways that contribute to the greater density and accelerated healing of Polynesian bon...

ba0001pp290 | Muscle, physical activity and bone | ECTS2013

Response of mechanically strained tenocytes to different cell culture substrates

Musson David , JungJoo Kim , Callon Karen , Naot Dorit , Shim Vickie , Anderson Iain , Cornish Jillian , Chhana Ashika

The musculoskeletal system experiences severe mechanical strain, with repetitive or extreme strains causing significant trauma; the result being an increase in mechanobiological studies evaluating mechanical strain on musculoskeletal cells. Currently, most stretching studies utilise fibronectin-coated cultures, as these enhance cell attachment. However, recent studies suggest that fibronectin increases cell turnover and DNA damage and affects cell differentiation. Furthermore,...

ba0001pp21 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Monosodium urate crystals inhibit tenocyte viability and function: implications for periarticular involvement in chronic gout

Chhana Ashika , Callon Karen , Dray Michael , Pool Bregina , Naot Dorit , Gamble Greg , Coleman Brendan , McQueen Fiona , Cornish Jillian , Dalbeth Nicola

Background: In patients with gout, urate deposition has been observed both adjacent to and within tendons, suggesting that monosodium urate monohydrate (MSU) crystals are likely to be in direct contact with tenocytes, the stromal cells of tendons. The aim of this study was to determine the effects of MSU crystals on tenocyte viability and function.Methods: Cultures of primary rat tenocytes were prepared from Wistar rat tails. Primary human tenocytes were...