Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp182 | Cell biology: osteoblasts and bone formation | ECTS2013

Evaluation of bone formation capacities of human adipose-derived stromal cells cultured in platelet growth factor-enriched plasma medium.

Guilloton Fabien , Rabani Vahideh , Brennan Meadhbh , Bassi Giulio , Krampera Mauro , Layrolle Pierre , Sensebe Luc , Deschaseaux Frederic

Human adipose-derived stromal cells (ASCs) exhibit strong plasticity and proliferation potentials. In addition, ASCs are easy to harvest and are found at high frequency in adipose tissue samples. This gives us opportunities for their use in bone regeneration therapy. We thus evaluated the bone formation potential of ASCs in vitro and in vivo.ASCs were isolated from subcutaneous adipose tissue (following Local Ethical Guideline and after...

ba0001pp178 | Cell biology: osteoblasts and bone formation | ECTS2013

Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue

Deschaseaux Frederic , Gaillard Julien , Langonne Alain , Chauveau Christophe , Naji Abderrahim , Bouacida Amina , Rosset Philippe , Heymann Dominique , de Pinieux Gonzague , Rouas-Freiss Nathalie , Sensebe Luc

Bone-marrow mesenchymal stem cells (MSCs) are the origin of bone-forming cells with immunomodulation potential. Among the generated immunosuppressive molecules there is HLA-G5. HLA-G proteins play a crucial role in promoting the acceptance of allografts. However, the mechanisms regulating the expression of HLA-G5 in human MSCs are unknown. We induced differentiation of human MSCs (harvested from iliac crests of healthy volunteers after their informed consent following approved...