Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp4 | Arthritis and other joint diseases: translational and clinical | ECTS2014

Phenotypic and functional characterization of osteoclast progenitors in circulatory and synovial compartments of patients with rheumatoid arthritis

Sucur Alana , Jajic Zrinka , Ikic Marina , Ivcevic Sanja , Kelava Tomislav , Grcevic Danka

Rheumatoid arthritis (RA) is marked by the persistent inflammation and osteodestruction, causing progressive disability. Osteoclast progenitors (OCPs) represent a subpopulation of peripheral blood monocytes and have been shown to be present in healthy subjects and arthritic patients. The aim of our study was to determine the phenotype, frequency and osteoclastogenic potential of OCPs in the peripheral blood and synovial fluid of RA patients in response to anti-TNF therapy....

ba0005p11 | Arthritis and other joint diseases: translational and clinical | ECTS2016

Chemotactic signals mediating osteoclast progenitor migration in collagen induced arthritis

Flegar Darja , Sucur Alan , Markotic Antonio , Kelava Tomislav , Kovacic Natasa , Ivcevic Sanja , Zrinski-Petrovic Katerina , Katavic Vedran , Grcevic Danka

Introduction: Collagen induced arthritis (CIA) is a mouse model of rheumatoid arthritis marked by persistent inflammation and enhanced osteoresorption at the affected joints as well as systemic osteopenia due to increased activation of osteoclast progenitors (OCP). Abundantly secreted chemokines presumably attract OCPs to the inflamed sites. Our goal was to identify chemotactic signals important for OCPs recruitment in CIA.Methods: After obtaining approv...

ba0003pp68 | Bone development/growth and fracture repair | ECTS2014

Increased expression of PTX3 in non-hematopoietic periosteal cells during fracture healing

Kelava Tomislav , Ivcevic Sanja , Katavic Vedran , Kovacic Natasa , Cvija Hrvoje , Petrovic Katerina Zrinski , Kuzmac Sania , Kalajzic Ivo , Bottazzi Barbara , Grcevic Danka

Pentraxin 3 (PTX3) is a highly conserved member of the long pentraxins subfamily produced in response to proinflammatory stimuli. Its biological roles have been associated to infection, female fertility and angiogenesis. We aimed to elucidate the role of PTX3 in the process of fracture healing.WT mice and mice deficient for the PTX3 gene (PTX3-KO) were used, after obtaining the approval from the Ethical committee. Cells from bone marrow and endosteal/per...

ba0005p10 | Arthritis and other joint diseases: translational and clinical | ECTS2016

Identifying cell populations coupling inflammation to osteoresorption in arthritis

Mosler Elvira Lazic , Flegar Darja , Sucur Alan , Fadljevic Martina , Kelava Tomislav , Markotic Antonio , Ivcevic Sanja , Zrinski-Petrovic Katerina , Katavic Vedran , Grcevic Danka , Kovacic Natasa

Rheumatoid arthritis (RA) is characterised by periarticular bone destruction leading to permanent disability. Some other forms of arthritis, such as arthritis in systemic lupus erythematosus (SLE), rarely produce bone lesions. Corresponding to that, inactivation of Fas produces a murine SLE-like phenotype, but alleviates bone loss during antigen-induced arthritis (AIA). Aim of this study was to identify cell populations differentially regulated in non-resorptive vs. resorptive...