Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp460 | Other diseases of bone and mineral metabolism | ECTS2013

Osteopontin ASARM peptide binding to crystal faces of hydroxyapatite – computational simulations

Mansouri Ahmad , Masica David , Gray Jeffrey , McKee Marc

ASARM peptide (acidic, serine- and aspartate-rich motif) and osteopontin (OPN) fragments accumulate in X-linked hypophosphatemia patients and/or in the Hyp mouse model and, when phosphorylated, potently inhibit mineralization in osteoblast cultures. To investigate this inhibition, we modeled the binding to hydroxyapatite of the human OPN-ASARM peptide (DDSHQSDESHHSDESDEL) using RosettaSurface computational simulations. Peptide binding to hydroxyapatite atomic planes constructe...