Searchable abstracts of presentations at key conferences on calcified tissues

ba0005p165 | Cell biology: osteoblasts and bone formation | ECTS2016

Canine osteoblasts from trabecular, cortical and subchondral bone present differences in alkaline phosphatase activity

Perpetuo Ines Pedro , Shah Mittal , Parsons Kevin , Orriss Isabel , Doube Michael , Pitsillides Andrew , Meeson Richard

Hip osteoarthritis is a cause of significant morbidity to people and their canine companions. Medical management is frequently insufficient, leading to surgery to relieve pain and regain mobility. Hip replacements are not without potential complications, including loosening and infection. Currently, there is a focus on uncemented implants to decrease these problems, however these rely on the biology of the femur for osseointegration and long-term stability. It has been previou...

ba0005p166 | Cell biology: osteoblasts and bone formation | ECTS2016

Primary osteoblast culture from domestic dog (Canis lupus familiaris)

Perpetuo Ines Pedro , Meeson Richard , Pitsillides Andrew , Doube Michael , Orriss Isabel

Effects of whole bone scaling on isolated osteoblast behaviour are unknown. With two orders of magnitude range in body mass, dog breeds are well-suited to determine such relationships.Femoral heads from three canine hip replacement surgeries were collected. Bone fragments were washed in PBS+AB/AM, trypsin-digested and incubated in 0.2% collagenase. Cells from resultant supernatant were seeded in DMEM+10% FCS+AB/AM at 37 °C, 5% CO2, g...