Searchable abstracts of presentations at key conferences on calcified tissues

ba0005ws1.1 | Preclinical imaging beyond bone mass | ECTS2016

LivE imaging for in vivo cellular profiling

Muller Ralph

The maintenance and adaptation of bone morphology results from orchestrated remodeling processes. These processes are locally coordinated by osteocytes with biochemical signals that result in increased or decreased bone formation or resorption activities. To better understand the morphology, we therefore have to understand how osteocytes determine dynamic morphometric parameters within their local microenvironment. Recently, a local in vivo environment (LivE)...

ba0001oc4.5 | Osteoblasts and osteocytes | ECTS2013

Mechanical loading increases the effect of sclerostin antibody treatment in a mouse model of high turnover osteoporosis

von Salis-Soglio Marcella , Kuhn Gisela , Kneissel Michaela , Muller Ralph

Sclerostin, a Wnt signaling antagonist encoded by the SOST gene, negatively regulates osteoblasts and inhibits bone formation. Mechanical loading, which induces bone formation, leads to a decrease in sclerostin levels. Recently, neutralizing antibodies against sclerostin were tested successfully for the treatment of osteoporosis in rodents. However, sclerostin is not the only signal involved in mechanotransduction. Therefore we investigated whether treatment with sclerostin an...

ba0001pp52 | Bone biomechanics and quality | ECTS2013

Bone morphometry from human peripheral quantitative computer tomography scans is preserved by virtual high-resolution image reconstruction

Schulte Friederike , Badilatti Sandro , Parkinson Ian , Goldhahn Jorg , Muller Ralph

Peripheral quantitative computed tomography (pQCT) is receiving considerable attention in the diagnosis and monitoring of human bone diseases. It is well accepted that lower image resolution compared to micro-computed tomography (micro-CT) affects bone morphometry. With advances in micro-CT evaluation techniques such as sample-specific remodeling simulations or dynamic bone morphometry, there is the potential to also allow the application of such techniques to clinical pQCT sc...

ba0001pp90 | Bone development/growth and fracture repair | ECTS2013

The choice of fetal bovine serum influences the degree of spontaneous mineralization on silk fibroin scaffolds in 3D cell cultures

Paulsen Samantha , Vetsch Jolanda , Muller Ralph , Hofmann Sandra

Silk fibroin (SF) sponges are a promising scaffold material for tissue engineering due to their biocompatibility, mechanical properties, and ability to support calcium-phosphate formation in vitro. However, previous studies have shown that SF can mineralize spontaneously in the presence of culture media, which has a detrimental effect on experimental integrity when analyzing how cells deposit bone-like tissue in tissue engineering studies. In this study we analyzed th...

ba0001pp243 | Cell biology: osteocytes | ECTS2013

Single osteocyte gene expression in an in vivo model for load-induced bone adaptation

Wilson Robin , Trussel Andreas , Webster Duncan , Kurth Felix , Dittrich Petra , Muller Ralph

It is hypothesized that osteocytes regulate bone adaptation by sensing mechanical strains in their microenvironments and signaling net bone formation or resorption. Owing to bone’s anisotropic architecture, individual osteocytes within a bone experience varying strains under mechanical loading. Thus, to accurately determine the relationships between mechanical strain, osteocyte behavior, and bone remodeling, it is crucial to use a single-cell approach. Using an in viv...

ba0001pp437 | Osteoporosis: treatment | ECTS2013

The spatial relationship between bone formation and bone resorption in healthy and ovariectomized mice treated with PTH, bisphosphonate or mechanical loading

Ruffoni Davide , Weigt Claudia , Fattorini Elisa , Levchuk Alina , Schulte Friederike , Kuhn Gisela , Muller Ralph

Bone is continuously remodeled to remove damage, to adapt to changes in mechanical demands and to regulate calcium homeostasis. The first aim is accomplished by coupled bone formation and resorption whereas adaptation requires sites of formation to differ from those of resorption. The regulation of circulating ions is achieved by a stochastic exchange of bone packets. Here, we investigated these different aspects of remodeling in healthy and ovariectomized (OVX) mice treated w...

ba0005p76 | Bone development/growth and fracture repair | ECTS2016

Multicentre study reveals poor correlation between in vitro and in vivo assessments of biomaterials for bone-regeneration

Billstrom Gry Hulsart , Dawson Jon , Hofmann Sandra , Muller Ralph , Stoddart Martin , Alini Mauro , Redl Heinz , Haj Alicia El , Brown Robert , Salih Vehid , Hilborn Jons , Larsson Sune , Oreffo Richard

Introduction: Research on biomaterials for bone regeneration generates a plethora of new biomaterials requiring evaluation with reliable and comparable methods of biocompatibility and functionality for clinical translation. To reduce the burden of in vivo assessment, there is a need for refined in vitro assays that are predictive of in vivo outcomes. This retrospective study correlated in vitro results with in vivo outcomes observed...