Searchable abstracts of presentations at key conferences on calcified tissues

ba0005cabs.oc2.3 | Oral Communications | ECTS2016

Cancer cell homing to the bone marrow is modulated by the mesenchymal stromal cell

Rossnagl Stephanie , Nakchbandi Inaam

Several cell types form the hematopoietic stem cell niche. These niches sometimes become hijacked by cancer cells, which may later form metastatic lesions. Our aim was therefore to characterize the bone marrow microenvironment to affect cancer cell homing to bone marrow.Pharmacologic modification consisting of PTH to stimulate the osteoblasts and zoledronic acid to prevent the increase in osteoclasts through PTH resulted in increased homing of cancer cel...

ba0005p168 | Cell biology: osteoblasts and bone formation | ECTS2016

Two different domains of fibronectin stimulate osteoblast differentiation by activating distinct integrins

Sens Carla , Rau Katrin , Nakchbandi Inaam

Fibronectin is produced in different isoforms by osteoblasts during differentiation. In contrast to the circulating Isoform, which lacks extra domains, the fibronectin laid down by the osteoblasts in the matrix contains one or both extra-domains called EDA and EDB. We had found that deletion of fibronectin in osteoblasts decreased BMD. This is mediated by diminished osteoblast differentiation and cannot be corrected by the circulating isoform of fibronectin, suggesting a role ...

ba0005cabs.oc3.2 | Oral Communications | ECTS2016

Osteoblasts inhibit the immune response against cancer

Rossnagl Stephanie , Kraft Sabrina , Sens Carla , Nakchbandi Inaam

Osteoblasts line the inner surface of the bone and are located in close proximity to the bone marrow, where hematopoiesis takes place. Osteoblasts produce several cytokines that affect various steps of hematopoiesis, and produce fibronectin isoforms that affect cell differentiation. Little is known however on whether osteoblasts modulate the immune response. Our aim was to evaluate the role of fibronectin originating from the osteoblasts on hematopoiesis and the immune respons...

ba0005p455 | Other diseases of bone and mineral metabolism | ECTS2016

An isoform of fibronectin is responsible for bone loss in hepatic osteodystrophy and its deleterious effects can be prevented by binding the mediating receptor

Sens Carla , Rau Katrin , Altrock Eva , Klemis Verena , Tiwari Sanjay , Nakchbandi Inaam

Cholestatic liver disease is often associated with increased fracture risk. We had found that circulating levels of a fibronectin isoform called oncofetal fibronectin (oFN) were elevated in patients with primary biliary cirrhosis. Indeed, injecting oFN to mice suppressed osteoblast differentiation and bone mineral density in vivo, suggesting it is responsible for bone loss in these patients. The aim of this study was to define the mechanism by which oFN affects osteob...