Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp116 | Cell biology: osteoblasts and bone formation | ECTS2014

Inhibitory effect of GH on the adipogenic commitment of mesenchymal stromal cells derived from human trabecular bone

Bolamperti Simona , Spinello Alice , Sauer Aisha , GianLuigi Moro , Ometti Marco , Fraschini Gianfranco , Guidobono Francesca , Rubinacci Alessandro , Villa Isabella

Besides its well known effect on longitudinal bone growth, GH plays a role in the maintenance of adult bone mass. As aging progresses GH levels decline, bone mass decreases and mesenchymal precursors show a reduced osteogenic differentiation capacity resulting in an increase in bone marrow adipocytes. We investigated the effect of GH on MSC differentiation and the possible involvement of microRNA in this process. Human MSC derived from trabecular specimens, waste material of o...

ba0005p155 | Cell biology: osteoblasts and bone formation | ECTS2016

L-Carnitine facilitates mitochondrial activity and osteogenic differentiation in human primary osteoblast culture

Vacante Fernanda , Montesano Anna , Spinello Alice , Bolamperti Simona , Senesi Pamela , Terruzzi Ileana , Luzi Livio , Villa Isabella , Rubinacci Alessandro

Osteoblasts have a high rate of energy consumption during bone formation and bone protein synthesis. Therefore, it is likely that decreased energy production that accompanies aging, could contribute to reduced osteoblast activity, a critical feature of senile osteoporosis, and that this reduction might be counteracted by favoring energy availability. Cells of the osteoblastic lineage generate 40–80% of the requested energy through fatty acid degradation, thus the modulati...

ba0005p157 | Cell biology: osteoblasts and bone formation | ECTS2016

Evidence for an osteogenic activity of betaine in human osteoblast in culture

Spinello Alice , Montesano Anna , Senesi Pamela , Villa Isabella , Bolamperti Simona , Vacante Fernanda , Luzi Livio , Rubinacci Alessandro , Terruzzi Ileana

Betaine (BET) is a component of many food. It is an essential osmolyte and a source of methyl groups. BET consumed from food sources and through dietary supplements presents similar bioavailability. BET exerts an antioxidant activity and decreases inflammation states. Dietary supplement with BET are used in many inflammation-connected pathologies, although its mechanism of action is not fully understood. Recent studies have shown that BET stimulates muscle fibers differentiati...