Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp124 | Cell biology: osteoblasts and bone formation | ECTS2014

MiR-320a and miR-483-5p are over-expressed in osteoblasts from osteoporotic fractured hips

Garcia-Giralt Natalia , De-Ugarte Laura , Balcells Susana , Arino-Ballester Sergi , Yoskovitz Guy , Guerri Roberto , Mellibovsky Leonardo , Urreizti Roser , Nogues Xavier , Grinberg Daniel , Diez-Perez Adolfo

MicroRNAs are important regulators of gene expression with documented role in bone metabolism and osteoporosis. Moreover, the use of miRNAs constitutes potential therapeutic targets. Our aim was to identify miRNAs differentially expressed in fractured compared to healthy bone. Additionally, we performed a miRNA profiling of primary osteoblasts to assess the origin of the differentially expressed miRNAs. Total RNA was extracted from fresh femoral neck trabecular bone from women...

ba0001pp277 | Genetics | ECTS2013

A genomic and transcriptomic approach to the high bone mass phenotype: evidences of heterogeneity and of additive effects of TWIST1, IL6R, DLX3, and PPARG

Sarrion Patricia , Mellibovsky Leonardo , Urreizti Roser , Civit Sergi , Cols Neus , Garcia-Giralt Natalia , Yoskovitz Guy , Aranguren Alvaro , Malouf Jorge , del Rio Luis , Guerri Roberto , Nogues Xavier , Diez-Perez Adolfo , Grinberg Daniel , Balcells Susana

The aims of this study were to establish the prevalence of the high bone mass (HBM) phenotype in a cohort of Spanish postmenapausal women (BARCOS); to determine whether any of the HBM cases carry LRP5 or DKK1 mutations; to test the hypothesis of an inverse correlation between the number of common variant risk alleles and HBM; and to characterize the expression of osteoblast-specific and Wnt pathway genes in primary osteoblast RNA samples from two HBM cases.</...

ba0001pp278 | Genetics | ECTS2013

Gene-wide association study of RANK and RANKL genes in the bone context: functional study of BMD-associated SNPs

Garcia-Giralt Natalia , Yoskovitz Guy , Rodriguez-Sanz Maria , Urreizti Roser , Guerri Roberto , Arino-Ballester Sergi , Prieto-Alhambra Daniel , Mellibovsky Leonardo , Grinberg Daniel , Nogues Xavier , Balcells Susana , Diez-Perez Adolfo

Over the past decade, many GWAs and meta-analyses were performed to identify genes and regions involved in bone metabolism and in the osteoporotic phenotypes. Nevertheless, the majority of these GWAS results were not tested at any functional level. This study aims to find and study functional regions in the RANK and RANKL genes that encode well-established proteins in the bone remodeling equilibrium. SNPs, chosen for their location in an evolutionary conserve...