Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp135 | Cell biology: osteoblasts and bone formation | ECTS2014

High-resolution molecular validation of self-renewal and spontaneous differentiation in adipose-tissue derived human mesenchymal stem cells cultured in human platelet lysate

Dudakovic Amel , Camilleri Emily , Riester Scott , Westendorf Jennifer , Kakar Sanjeev , Dietz Allan , van Wijnen Andre

Improving the effectiveness of adipose-derived human mesenchymal stromal/stem cells (AMSCs) for skeletal therapies requires a detailed molecular characterization of mechanisms supporting cell proliferation and multi-potency of AMSCs. We compared gene expression data obtained by high-throughput RNA sequencing and RT-qPCR of actively proliferating and uninduced post-proliferative AMSCs with flow cytometry data for mesenchymal stem cell markers (e.g., CD44, CD73/NT5E, CD90/THY1 a...

ba0003pp96 | Cancer and bone: basic, translational and clinical | ECTS2014

Osteoblast-secreted extracellular vesicles stimulate the expansion of CD34+ human umbilical cord blood cells

Morhayim Jess , Demmers Jeroen , van Wijnen Andre , Braakman Eric , Cornelissen Jan , de Peppel Jeroen van , van Leeuwen Hans

Introduction: Umbilical cord blood (UCB) is increasingly used in hematopoietic stem cell (HSC) transplantations; however, the low cell numbers are still remaining as a limiting factor for proper engraftment. Osteoblasts are major constituents of HSC niche and play important roles in regulating HSC self-renewal and differentiation. Recently, extracellular vesicles (EVs) have been implicated in stem cell fate regulation via horizontal transfer of proteins and nucleic acids betwe...

ba0005cabs.op2.1 | Oral Poster Talks | ECTS2016

Antagonizing Mir-218 prevents breast cancer-induced osteolytic disease

Taipaleenmaki Hanna , van Wijnen Andre J , Stein Janet L , Hesse Eric , Stein Gary S , Lian Jane B

Signaling pathways crucial in bone development, including Wnt, are also upregulated in breast cancer cells to promote tumor growth in the skeleton, a process known as osteomimicry. Thus, we hypothesized that bone metastatic tumor cells also aberrantly express osteogenic miRNAs to support osteomimetic properties. We have previously shown that miR-218 is highly expressed in osteoblasts and promotes osteogenic differentiation. Interestingly, expression analysis revealed a signifi...

ba0003pp113 | Cell biology: osteoblasts and bone formation | ECTS2014

Bone-anabolic effects of sulforaphane, a naturally occurring isothiocyanate on bone metabolism

Thaler Roman , Dudakovic Amel , Rucci Nadia , Maurizi Antonio , Sturmlechner Ines , Spitzer Silvia , Rumpler Monika , Van Wijnen Andre J , Teti Anna , Klaushofer Klaus , Varga Franz

Few drugs generate bone-stimulatory effects via epigenetic mechanisms. Modulation of CpG-residues hydroxymethylation in gene-promoters of key osteoblast-related factors (e.g., DLX5) induces their expression and increases osteoblast differentiation in vitro. The chemical properties of sulforaphane (SFN), a natural compound abundantly present in cruciferous vegetables like broccoli, suggest that it may have similar molecular and biological effects. Previous stu...

ba0003pp115 | Cell biology: osteoblasts and bone formation | ECTS2014

Epigenetic modifications and canonical WNT signaling enable direct programming of non-osteogenic cells into osteoblasts

Cho Young-Dan , Yoon Won-Joon , Woo Kyung-Mi , Baek Jeong-Hwa , Lee Gene , van Wijnen Andre J , Ryoo Hyun-Mo

Mesenchymal cells alter and retain their phenotype during skeletal development through activation or suppression of signaling pathways. For example, we have shown that Wnt3a only stimulates osteoblast differentiation in cells with intrinsic osteogenic potential (e.g., MC3T3-E1 pre-osteoblasts) and not in fat cell precursors or fibroblasts (respectively, 3T3-L1 pre-adipocytes or NIH3T3 fibroblasts). Wnt3a promotes osteogenesis in part by stimulating autocrine production of the ...