Searchable abstracts of presentations at key conferences on calcified tissues

ba0001oc4.4 | Osteoblasts and osteocytes | ECTS2013

Glucocorticoid exposure reduces expression of sclerostin in bone marrow stromal cells

Thiele Sylvia , Rauch Alexander , Tuckermann Jan P , Hofbauer Lorenz C , Rauner Martina

Glucocorticoids (GC) are effective drugs in the treatment of inflammatory diseases, including various forms of arthritis. However, their use is limited by negative effects on bone mass and strength, resulting in increased osteoporotic fractures. Conditional knockout mice demonstrated that the GR in osteoblasts is essential for GC-dependent bone loss. Recent studies show that GC profoundly inhibit Wnt signaling by stimulating the expression of Wnt antagonists such as dickkopf-1...

ba0003pp27 | Bone biomechanics and quality | ECTS2014

Long term treatment with odanacatib maintains normal trabecular biomechanical properties in ovariectomized adult monkeys as demonstrated by micro-CT based finite element analysis of the vertebral cores

Cabal Antonio , Jayakar Richa Y , Zhang Jingru , Sardesai Swanand , Williams Donald S , Duong Le T

The cathepsin K inhibitor odanacatib (ODN) is a bone formation-sparing inhibitor of osteoclastic resorption activity. This drug is currently under development for the treatment of postmenopausal osteoporosis. To support the bone safety profile of ODN, we evaluated the effects of ODN on trabecular bone hard tissue properties in the estrogen-deficient model of the ovariectomized (OVX) rhesus monkeys. Animals (n=16/group, age 11–22 years) were treated immediately af...

ba0006oc23 | (1) | ICCBH2017

Sustained radiographic and functional improvements with asfotase alfa treatment from up to 7 years in children with hypophosphatasia

Whyte Michael P. , Rockman-Greenberg Cheryl , Moseley Scott , Denker Andrew E. , McAlister William H.

Objective: Children with hypophosphatasia (HPP) treated with asfotase alfa in a Phase 2 study (NCT00952484) and its open-label extension (NCT01203826) experienced significant improvements in skeletal mineralization and physical function that were sustained through 5 years of treatment (1). Herein, we report data from these studies with a maximum of 7 years of treatment.Methods: Children with HPP aged 6–12 years at baseline received asfotase alfa (3 ...

ba0006p107 | (1) | ICCBH2017

A case of a novel de novo PLS3 deletion, presenting with vertebral fractures and mild dysmorphism

Doulgeraki A. , Costantini A. , Kampe A. , Karavitakis E. , Jantti N. , Krallis P. , Athanasopoulou H. , Xaidara A. , Makitie O.

Background: Mutations in the PLS3 gene, encoding plastin 3, cause X-linked osteoporosis. Osteoporosis is characterized by low bone mineral density (BMD) and increased susceptibility to fractures. Here we describe a 7-year-old boy with osteoporosis due to a novel PLS3 deletion.Presenting problem: The patient, born to non-consanguineous parents, had a history of one low-energy long-bone fracture, three vertebral fractures (T5, T6 T8) and kyphosis. DXA scan...

ba0005p228 | Energy metabolism and bone, fat and bone | ECTS2016

Effects of roux-en-Y gastric bypass surgery on bone quality: a pilot study

Hogestol I.K. , Paschalis E.P. , Gamsjaeger S. , Hassler N. , Shabestari M.G. , Gulseth H.L. , Mala T. , Klaushofer K. , Eriksen E.F.

Roux-en-Y gastric bypass surgery (RYGBP) is one of the leading surgical treatments for morbid obesity and leads to significant long-term weight loss, diabetes remission, decreased cardiovascular events, and reduced mortality. RYGBP is, however, also implicated in increased fracture risk, mainly due to higher bone turnover rates and malabsorption. In the present study we used Raman microspectroscopic analysis to determine bone quality (an important determinant of bone strength)...

ba0003pp223 | Osteoporosis: evaluation and imaging | ECTS2014

Bone mineral density and bone turnover markers in patients with thyroid cancer and L-T4 suppressive therapy after 25 years of follow-up

de Mingo Dominguez Maria Luisa , Iglesias Sonsoles Guadalix , Alvarez Maria Begona Lopez , Diaz-Guerra Guillermo Martinez , Carranza Federico Hawkins

Background: Patients with differentiated thyroid cancer (DTC) are treated with L-thyroxine (L-T4) in relatively hight doses to suppress endogenous TSH levels. Very long-term effect of thyroid hormones supplementation on bone are controversial.Objetive: To study the possible negative effects on bone mineral density (BMD) and bone markers in DTC patients followed in our center.Methods: A 46 po...

ba0006oc25 | (1) | ICCBH2017

Biochemical and physical function outcomes after 5 years of treatment with asfotase alfa in adolescents and adults with hypophosphatasia: phase 2 study results

Kishnani Priya S. , Rockman-Greenberg Cheryl , Denker Andrew E. , Moseley Scott , Whyte Michael P.

Objective: To evaluate safety and efficacy after 5 years of treatment with asfotase alfa in adolescents and adults with hypophosphatasia (HPP) in a Phase 2, open-label, randomized, dose-ranging study (NCT01163149).Methods: Treatment with subcutaneous asfotase alfa 0.3 or 0.5 mg/kg per d was compared with no treatment (control) for 6 months in patients aged 13–66 years. After 6 months, all patients (treatment and control groups) received active treat...

ba0002p22 | (1) | ICCBH2013

Association of volumetric bone mineral density, bone morphometry and trabecular bone micro-architecture with leptin and soluble leptin receptor in adolescent idiopathic scoliosis

Tam Elisa M S , Yu Fiona W P , Hung Vivian W Y , Liu Zhen , Lam Tsz-Ping , Liu King Lok , Ng Bobby K W , Lee Simon K M , Qiu Yong , Cheng Jack C Y

Objectives: Low bone mass in adolescent idiopathic scoliosis (AIS) has been well reported, however the etiologies of the disease and this abnormal phenotype were still unknown. Leptin have profound effects on bone metabolism and skeletal growth, and was speculated to play a role in the etiopathogenesis of AIS. The objective of this study was to investigate the bone quality in AIS and its association with leptin and soluble leptin receptor (sOB-R).Methods...

ba0001pp127 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Hypercalcemia following high vitamin D loading dose

Lips Paul , Neradova Aegida , van Schoor Natasja , Vervloet Mark

Vitamin D deficiency is common in older persons and non-western immigrants. Vitamin D is often started in loading doses of 50 000 IU/ml solution. Though, generally considered safe, this highly concentrated solution carries some risks as is illustrated by the following cases. A woman of >80 years old was admitted with hypercalcemia, calcium 3.27 mmol/l. She complained of nausea, thirst and polyuria for 3 months. History included cholecystitis, atrial fibrillation, myocardia...

ba0005p109 | Cancer and bone: basic, translational and clinical | ECTS2016

Antiproliferative properties of oleuropein in human osteosarcoma cells

Moran Jose M , Leal-Hernandez Olga , Canal-Macias Maria L , Lavado-Garcia Jesus M , Roncero-Martin Raul , Aliaga Ignacio , Pedrera-Zamorano Juan D

Background: Cancer is one of the leading causes of death worldwide. Natural products have been regarded as important sources of potential chemotherapeutic agents. In this study, we evaluated the antiproliferative activity of oleuropein, an olive oil compound traditionally found in the Mediterranean diet.Design and Methods: The antiproliferative activity on two human osteosarcoma cell lines (MG-63 and Saos2) was evaluated in vitro using the MTT c...