Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp157 | Cancer and bone: basic, translational and clinical | ECTS2013

Bone metastatic prostate cancer cells regulate their growth via impairing osteoblast differentiation

van Driel Marjolein , Robbesom Iris , Koster Ruben , Boers-Sijmons Bianca , Chiba Hideki , van Leeuwen Hans

Metastases to the bone are the incurable final outcomes of cancer, reducing both length and quality of life in an aggressive way. Despite the discoveries of many factors involved, no cure has been found. Metastatic outgrow starts in interaction with the bone micro-environment. The first attachment in bone is with the osteoblasts lining the endosteal surface. Our aim is to study the role of the osteoblasts in metastatic tumor spread and growth.We used an ...

ba0006p025 | (1) | ICCBH2017

The impact of Haemophilia A on bone health

Doulgeraki Artemis , Xafaki P. , Pergantou H. , Athanasopoulou H. , Platokouki H.

Objectives: Haemophilia A (FVIII deficiency) is an X-linked disorder of haemostasis with bleeding tendency, mainly in joints and muscles. Recurrent haemarthroses, subsequent immobilization and avoidance of contact sports, may affect these patients’ skeletal health.Methods: Evaluation of bone health was performed in 51 children with Haemophilia A (severe: 41, all on prophylaxis treatment), mean age: 11.7±3.6 years. Dual-energy X-Ray absorptiomet...

ba0005p213 | Chondrocytes and cartilage | ECTS2016

The vacuolar H+ ATPase V0 subunit D2 is associated with chondrocyte hypertrophy and supports chondrocyte differentiation

Ayodele Babatunde , Mirams Michiko , Pagel Charles , Mackie Eleanor

In a recent unbiased transcriptomic study of genes associated with equine osteochondrosis, we identified several novel cartilage genes. The current study was undertaken to determine whether these genes are regulated during chondrocyte hypertrophy, and to identify novel hypertrophy-associated genes for further study in vitro. Gene expression was investigated by quantitative PCR (qPCR) in different zones of growth cartilage microdissected from equine foetal metatarsal b...

ba0003oc3.6 | Osteoclasts, gastric hormones and HIF | ECTS2014

Skeletal effects of the gastrin receptor antagonist netazepide in H+/K+ATPase beta-subunit deficient mice

Aasarod Kristin Matre , Koldeh Masoud Ramezanzadeh , Mosti Mats Peder , Stunes Astrid Kamilla , Viggaklev Bjorn Ivar , Reseland Janne Elin , Beisvag Vidar , Sandvik Arne Kristian , Skallerud Bjorn Helge , Syversen Unni , Fossmark Reidar

Epidemiological studies suggest that patients using proton pump inhibitors (PPIs) have increased fracture risk. We have previously shown that H+/K+ATPase beta-subunit knockout (KO) mice have reduced BMD, BMC and mechanical bone strength compared to WT. Like users of PPIs, these mice have elevated serum gastrin levels due to high gastric pH. We wanted to study whether elevated gastrin influences bone quality in these mice.Female KO and WT mice aged 6 week...

ba0006p051 | (1) | ICCBH2017

Low bone density and fragility fractures in unbalanced translocation T(9;11)

Vai Silvia , Broggi Francesca , Bianchi Maria Luisa

Background: Trisomy 9p is a rare abnormality caused by duplication of the short arm of chromosome 9. Translocation t(9;11) is a rarer variant. Both anomalies are compatible with long survival. Clinical manifestations are very variable, and include short height, mental retardation, hypertelorism, strabismus, foot/hand anomalies, delayed bone maturation. Low bone mineral density (BMD) or fragility fractures have never been reported.Presenting prob...

ba0003pp96 | Cancer and bone: basic, translational and clinical | ECTS2014

Osteoblast-secreted extracellular vesicles stimulate the expansion of CD34+ human umbilical cord blood cells

Morhayim Jess , Demmers Jeroen , van Wijnen Andre , Braakman Eric , Cornelissen Jan , de Peppel Jeroen van , van Leeuwen Hans

Introduction: Umbilical cord blood (UCB) is increasingly used in hematopoietic stem cell (HSC) transplantations; however, the low cell numbers are still remaining as a limiting factor for proper engraftment. Osteoblasts are major constituents of HSC niche and play important roles in regulating HSC self-renewal and differentiation. Recently, extracellular vesicles (EVs) have been implicated in stem cell fate regulation via horizontal transfer of proteins and nucleic acids betwe...

ba0007p172 | (1) | ICCBH2019

Cow's milk allergic infants on amino acid-based medical nutrition formula maintain adequate serum concentrations of phosphorus, calcium and magnesium despite the use of acid-suppressive medication

Harvey Bryan M , Eussen Simone RBM , Helvoort Ardy van , Harthoorn Lucien F

Objectives: We recently demonstrated that cow’s milk allergic infants who received an amino acid-based formula (AAF) for 16 weeks as oral feeding had adequate mineral status (1). One factor that may negatively affect mineral solubility and bioavailability and hence mineral status, is high gastric pH (2), but data on this in infants are lacking. Therefore, the present study evaluates serum concentrations of phosphorus, calcium and magnesium in a subgroup of infants on AAF ...

ba0002p120 | (1) | ICCBH2013

Osteoblasts communicate with their neighbouring cells via extracellular vesicles

Morhayim Jess , Demmers Jeroen , de Jong Ton , Braakman Eric , de Peppel Jeroen van , Cornelissen Jan , van Leeuwen Hans

Objectives: Strong coordination between osteoblasts and bone marrow cells is fundamental for the regulation of healthy bone turnover and other (patho)physiological processes. Extracellular vesicles (EVs) mediate communication between cells via horizontal transfer of proteins and nucleic acids. Osteoblasts secrete EVs in the form of matrix vesicles involved in bone mineralization, however, information about a role in intercellular communication is still lacking. In this study, ...

ba0006oc12 | (1) | ICCBH2017

Fracture incident rate and growth in a nationwide cohort of boys with Duchenne Muscular Dystrophy

Joseph S , Bushby K , Guglieri M , Horrocks I , Straub V , Ahmed SF , Wong SC , Northstar Clinical Network SC

Background: Fracture incidence rate and growth according to different glucocorticoid (GC) regimen in Duchenne Muscular Dystrophy (DMD) is currently unknown.Objective: To determine the extent of skeletal morbidity and the influence of GC regimen on fracture incidence rate and growth in a contemporary cohort of DMD in the UK.Method: Clinical details of 832 boys with DMD in the North Star database (2006–2015) from 23 centres were...

ba0004p164 | (1) | ICCBH2015

Effect of vitamin D supplementation on glucose metabolism, immune function and bone turnover in children with vitamin D deficiency

N El Fakhri , J McNeilly , M McMillan , M Le Brocq , Boroujerdi M , Halsey C , Ahmed S F , H McDevitt

Objectives: To assess the effects of short-term vitamin D supplementation on bone metabolism, glycaemic status and immune function in vitamin D deficient children.Method: Treatment with daily 5000 IU cholecalciferol supplementation for 6 weeks. At baseline and end of treatment serum 25 hydroxyvitamin D (25(OH)D), parathyroid hormone (PTH), alkaline phosphatase (ALP), serum collagen type 1 cross-linked C-telopeptide (CTX), serum calcium, HbA1c, sex hormon...