Searchable abstracts of presentations at key conferences on calcified tissues
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European Calcified Tissue Society Congress 2013

Lisbon, Portugal
18 May 2013 - 22 May 2013

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European Calcified Tissue Society Congress 2013, 18 - 22 May 2013; Lisbon, Portugal

Oral Communications

Osteoblasts and osteocytes

ba0001oc4.1 | Osteoblasts and osteocytes | ECTS2013

High-throughput DEXA and micro-CT screening in gene knockout mice identifies bone mass phenotypes

Brommage Robert , Liu Jeff , Kirkpatrick Laura , Powell David , Vogel Peter

Screening gene function in vivo is a powerful approach to discover novel drug targets in the human genome (Nat Rev Drug Discov 2 38–51, 2003). We present data for 3776 distinct gene knockout (KO) mouse lines with viable adult homozygous mice generated using both gene-trapping and homologous recombination technologies. Bone mass was determined from PIXImus DEXA scans of male and female mice at 14 weeks of age and by microCT analyses of bones from ...

ba0001oc4.2 | Osteoblasts and osteocytes | ECTS2013

The p38α MAPK pathway in osteoblasts contributes to ovariectomy-induced bone loss by upregulating interleukin 6 expression

Thouverey Cyril , Caverzasio Joseph

Selective p38α inhibitors have been found to prevent bone loss induced by estrogen deficiency but implicated mechanisms remained to be identified. The p38 MAPK pathway has been suggested to influence bone resorption at different regulatory levels. In osteoblasts, p38α has been reported to be involved in the production of osteoclastogenic interleukin 6 and Rankl in response to various bone-resorptive agents in vitro. Therefore, we investigated whether p38&#94...

ba0001oc4.3 | Osteoblasts and osteocytes | ECTS2013

Severe osteopenia, increased bone marrow adipogenesis, and fibronectin matrix changes in mice lacking both TG2 and FXIIIA transglutaminases

Mousa Aisha , Cui Cui , Song Aimei , Myneni Vamsee , Li Jingjing , Melino Gerry , Dickneite Gerhard , Murshed Monzur , Kaartinen Mari

Osteoblasts produce protein-crosslinking enzymes, transglutaminase 2 (TG2) and factor XIIIA (FXIIIA), which regulate fibronectin matrix stabilization and osteoblast differentiation in vitro. To examine if they are important in bone remodeling and in maintenance of bone quality and mass in vivo, we performed skeletal phenotyping of Tgm2−/− and F13a1−/− mice and generated a double-null Tgm2</em...

ba0001oc4.4 | Osteoblasts and osteocytes | ECTS2013

Glucocorticoid exposure reduces expression of sclerostin in bone marrow stromal cells

Thiele Sylvia , Rauch Alexander , Tuckermann Jan P , Hofbauer Lorenz C , Rauner Martina

Glucocorticoids (GC) are effective drugs in the treatment of inflammatory diseases, including various forms of arthritis. However, their use is limited by negative effects on bone mass and strength, resulting in increased osteoporotic fractures. Conditional knockout mice demonstrated that the GR in osteoblasts is essential for GC-dependent bone loss. Recent studies show that GC profoundly inhibit Wnt signaling by stimulating the expression of Wnt antagonists such as dickkopf-1...

ba0001oc4.5 | Osteoblasts and osteocytes | ECTS2013

Mechanical loading increases the effect of sclerostin antibody treatment in a mouse model of high turnover osteoporosis

von Salis-Soglio Marcella , Kuhn Gisela , Kneissel Michaela , Muller Ralph

Sclerostin, a Wnt signaling antagonist encoded by the SOST gene, negatively regulates osteoblasts and inhibits bone formation. Mechanical loading, which induces bone formation, leads to a decrease in sclerostin levels. Recently, neutralizing antibodies against sclerostin were tested successfully for the treatment of osteoporosis in rodents. However, sclerostin is not the only signal involved in mechanotransduction. Therefore we investigated whether treatment with sclerostin an...

ba0001oc4.6 | Osteoblasts and osteocytes | ECTS2013

Periostin synergizes with osteocytes β-catenin to mediate the adaptive skeletal response to loading

Bonnet Nicolas , Ferrari Serge

Mechanical stimulation triggers periostin (Postn) expression in the periosteum and osteocytes (Oc), which downregulates Sost and activates β-catenin signaling. Hence the cortical bone response to loading is abolished in Postn−/− mice. Here we investigated the role of Oc β-catenin and its interaction with Postn on the bone biomechanical response. Postn−/− were bred with Oc-Ctnn−/− mice to generate Po...