Searchable abstracts of presentations at key conferences on calcified tissues
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European Calcified Tissue Society Congress 2013

Lisbon, Portugal
18 May 2013 - 22 May 2013

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European Calcified Tissue Society Congress 2013, 18 - 22 May 2013; Lisbon, Portugal

Poster Presentations

Cell biology: osteocytes

ba0001pp237 | Cell biology: osteocytes | ECTS2013

Nucleotide and mechanically induced ATP release pathways in osteocytes

Kringelbach Tina M , Novak Ivana , Schwarz Peter , Jorgensen Niklas Rye

Background: We have previously shown that MLO-Y4 osteocytes express a number of P2 receptors, respond to a broad range of nucleotides (e.g. UTP) by increasing intracellular calcium concentration and release ATP upon both mechanical and UTP stimulation. The aim of this study therefore is to investigate how the osteocytes release ATP and whether there is a difference in release pathway depending on the type of stimulus.Methods: ATP release was investigated...

ba0001pp238 | Cell biology: osteocytes | ECTS2013

Calcium Sensing Receptor is expressed on/in osteocyte-like MLOY4 culture and modulated by strontium ranelate

Aveline Priscilla C , Toumi Hechmi , Lespessailles Eric , Boudot Cedric , Mentaverri Romuald , Rochefort Gael Y , Benhamou Claude-Laurent

Introduction: The calcium sensing receptor presence (CaSR) at the surface of the osteocytes has never been clearly investigated. The CaSR are known to be express on osteoblasts. Osteocytes being old osteoblasts embedded in the matrix, this expression of CaSR is likely, and could constitute a key role to calcium signalling.Strontium ranelate (SrRan) has shown to activate osteoblasts by fixation on CaSR (Chattopadhyay N 2007, Biochem Pharmacol; Hu...

ba0001pp239 | Cell biology: osteocytes | ECTS2013

Glycosaminoglycans and their sulfate derivates differentially regulate the osteocytic phenotype of murine and rat osteocyte-like cell lines

Tsourdi Elena , Salbach-Hirsch Juliane , Rauner Martina , Richter Claudia , Rachner Tilman , Hofbauer Lorenz

Introduction: Collagen and glycosaminoglycans (GAGs) such as hyaluronan (HA) and chondroitin sulfate (CS) are basic elements of bone structure and collagen-GAG composites are currently developed for a wide range of applications. Here, we report on the molecular and cellular effects of GAGs and their sulfated derivatives on osteocytes, which are fundamental orchestrators of bone remodeling.Materials and methods: The effects of native and sulfate-modified ...

ba0001pp240 | Cell biology: osteocytes | ECTS2013

RANKL subcellular trafficking in osteocytes

Honma Masashi , Ikebuchi Yuki , Kariya Yoshiaki , Hayashi Madoka , Hayashi Naoki , Aoki Shigeki , Suzuki Hiroshi

RANKL is the central player in the regulation of osteoclastogenesis and the quantity of RANKL presented to osteoclast precursors is an important factor determining the magnitude of osteoclast formation. It had been believed for years that osteoblastic cells are the major source of RANKL presented to osteoclast precursors, and we have previously focused on RANKL intracellular behavior in osteoblastic cells. However, recent two reports controverted this traditional concept and s...

ba0001pp241 | Cell biology: osteocytes | ECTS2013

Inhibition of osteocyte-induced osteoclast precursor proliferation and migration by mechanical strain

Ko Seong-Hee , Lee Heesu

The osteocyte most likely plays a role in bone remodeling by instructing osteoclasts to remove bone at specific sites. This entire process includes recruitment, proliferation and differentiation of osteoclast precursors. And osteocytes are responsible for detecting and responding to mechanical strain and may send signal to other cells. Therefore, to determine the role for osteocytes and mechanical strain in bone remodeling, we examined the effect of steady or pulsatile shear s...

ba0001pp242 | Cell biology: osteocytes | ECTS2013

Activation of the parathyroid hormone-receptor is involved in the pro-survival effect of hypotonic shock in osteocyte-like MLO-Y4 Cells

Maycas Marta , Ardura Juan A , de Castro Luis Fernandez , Gortazar Arancha , Esbrit Pedro

The PTH type 1 receptor (PTH1R) is an important modulator of bone remodeling. In mice, PTH1R ablation in osteocytes produces trabecular bone reduction and impaired calcium homeostasis; meanwhile, its overexpression in these cells promotes periosteal and endocortical bone formation. Osteocytes can translate mechanical stimuli into bone-forming signals. Skeletal unloading induces osteocyte apoptosis and bone loss, whereas mechanical stimuli prevent osteocyte apoptosis through in...

ba0001pp243 | Cell biology: osteocytes | ECTS2013

Single osteocyte gene expression in an in vivo model for load-induced bone adaptation

Wilson Robin , Trussel Andreas , Webster Duncan , Kurth Felix , Dittrich Petra , Muller Ralph

It is hypothesized that osteocytes regulate bone adaptation by sensing mechanical strains in their microenvironments and signaling net bone formation or resorption. Owing to bone’s anisotropic architecture, individual osteocytes within a bone experience varying strains under mechanical loading. Thus, to accurately determine the relationships between mechanical strain, osteocyte behavior, and bone remodeling, it is crucial to use a single-cell approach. Using an in viv...

ba0001pp244 | Cell biology: osteocytes | ECTS2013

Strontium ranelate and conditioned medium from mechanically-stimulated human bone cells both enhance osteogenic differentiation of mesenchymal stem cells

Bakker Astrid , Zandieh-Doulabi Behrouz , Klein-Nulend Jenneke

Strontium ranelate (SrRan) is an efficient treatment for osteoporosis, because SrRan both inhibits osteoclasts and stimulates osteoblastic bone formation. We have previously shown that SrRan also affects mouse osteocyte signaling towards osteoclast precursors and mature osteoblasts. This study assessed the effect of SrRan on paracrine signaling from mechanically-stimulated human osteocytes towards mesenchymal stem cells.Human primary bone cells, used as ...

ba0001pp245 | Cell biology: osteocytes | ECTS2013

IGF1 regulates MC-3T3 and human primary osteoblast to osteocyte differentiation in 3D culture

Scully Nicole E E , Mason Deborah J , Evans Bronwen A J

Osteocytes differentiate from osteoblasts, are embedded in mineralised matrix and are critical regulators of bone remodelling. In vitro osteocyte models are limited to cell lines in monolayer, which do not represent their 3D environment in vivo. We have shown that osteoblasts in 3D gels differentiate along the osteocytic pathway. Since IGF1 regulates osteoblasts, and is involved in osteocyte response to mechanical loading, we hypothesised that IGF1 modulates ...

ba0001pp246 | Cell biology: osteocytes | ECTS2013

Development of a novel 3D mineralising culture system to investigate the differentiation of osteoblasts to osteocytes

Scully Nicole E E , Evans Sam L , Mason Deborah J , Evans Bronwen A J

Osteocytes make up >90% of bone cells, are embedded in mineralised matrix where they form a communication network. Osteocytes differentiate from osteoblasts, and are mechano-sensitive. They are very difficult to isolate with a dependence on cell lines for in vitro studies of osteocyte biology. Therefore new methods to study these cells are essential. Recent publications indicate that osteoblasts maintained in in vitro 3D collagen gels may differentiate to...