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Bone Abstracts (2013) 1 PP63 | DOI: 10.1530/boneabs.1.PP63

Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil.

Bone tissue engineering relies on the combination of scaffolds and cells aiming to heal bone defects. Among the many candidates for scaffolds, gelatin sponge (Gelfoam®) and fibrin glue (Tissucol®) are of interest due to the well-established biocompatibility and the ability to be loaded with cells. Here, we evaluated their potential to stimulate bone formation by microtomography analysis. For this, 5-mm unilateral calvarial defect was created in rats deeply anesthetized and filled with Gelfoam® (n=4) or Tissucol® (n=4). Untreated defects were used as control (n=7). At 4 and 8 weeks after surgery the animals were euthanized, the calvariae were harvested, fixed and analysed by microtomography for evaluation of bone volume and bone surface in the 5-mm defects. Data were compared by Kruskal–Wallis test (P<0.05). At 4 and 8 weeks bone volume in mm3 was 6.60±0.98 and 6.21±2.51 in Gelfoam® group, 6.53±3.52 and 9.30±6.62 in Tissucol® group, and 4.45±2.72 and 6.99±3.33 in control group, respectively. Bone surface in mm2 was 0.33±0.05 and 0.27±0.08 in Gelfoam® group, 0.32±0.13 and 0.45±0.27 in Tissucol® group, and 0.24±0.01 and 0.28±0.11 in control group at 4 and 8 weeks, respectively. There is no statistically significant difference in both bone volume and surface among the three groups. Furthermore, irrespective the treatment bone volume and surface were kept constant from 4 to 8 weeks. Despite the potential to act as scaffolds in bone tissue engineering procedures the present results show that neither Gelfoam® nor Tissucol® are capable of stimulating bone formation.

Financial support: FAPESP and CNPq.

Volume 1

European Calcified Tissue Society Congress 2013

Lisbon, Portugal
18 May 2013 - 22 May 2013

European Calcified Tissue Society 

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