Objective: Oosteoporoti fractures are a growing problem especially in aged societies of industrialized countries. Therefore, a clinical demand for synthetic bone graft substitutes is increasing. Despite the general success CPC showed clinically, development of CPC-based material by adding stronsium could improve its suitability to treat osteoporotic fractures.
Methods: SpragueDawley rates model of induced osteoporosis via multi-deficiencies diet combined with bilateral ovariectomy were utilized. A 4 mm wedge-shaped metaphyseal osteotomy was introduced in the left femur. CPC and CPC/strontium were compared with an empty defect in their ability to remedy osteoporotic fracture after 6 weeks. Morphological evaluation by means of μCT and hisotomorphometry on Movat pentachrom staining was performed. TRAP staining was carried out to evaluate catabolism at the fracture site. Samples were not normally distributed, therefore a KruskalWallis Test was used with 0.05 significance cutoff.
Results: μCt evaluation of fracture healing indicated increases in bone mineral density and bone volume in CPC/strontium group compared to CPC healing groups, both were higher than the empty defect group. Histomorphometry confirmed the bone formation results of the μCT analysis. Moreover, CPC group showed significantly higher soft tissue and cartilaginous tissue area than the CPC/strontium one. However, both were significantly lower the empty defect group. CPC/strontium group had a higher osteoclasts count than CPC group, which was in turn higher than the empty defect.
Discussion: CPC/strontium treatment showed an enhanced healing of osteoporotic fractures after 6 weeks than CPC alone. CPC/strontium appears to increase osteoclasts activity, which is needed for degradation of the implant. Moreover, the smaller cartilage fraction seen in CPC/strontium group suggests a positive effect on endochondral ossification. Furthermore, the currently ongoing analysis of bone anabolism and biomechanical competence would indicate strontiums effects on other aspects of bone healing.
18 May 2013 - 22 May 2013