Searchable abstracts of presentations at key conferences on calcified tissues
Previous issue | Volume 3 | ECTS2014 | Next issue

European Calcified Tissue Society Congress 2014

Prague, Czech Republic
17 May 2014 - 20 May 2014

Card image cap
European Calcified Tissue Society Annual Congress, 17 - 20 May 2014; Prague, Czech Republic

Oral Communications

Osteoclasts, gastric hormones and HIF

ba0003oc3.1 | Osteoclasts, gastric hormones and HIF | ECTS2014

HIF prolyl hydroxylase 2 (PHD2) controls bone homeostasis through HIF2α -- a novel player in osteohematology

Rauner Martina , Franke Kristin , Hofbauer Lorenz C , Wielockx Ben

Prolyl hydroxylase 2 (PHD2) regulates hypoxia-inducible factor α (HIFα) transcription factors and thus, erythropoietin (EPO) production. Under normoxic conditions, HIFα is constantly inactivated through hydroxylation by PHD2. Due to the embryonic lethality of PHD2 knock-out mice, its precise role in erythropoiesis and tissue homeostasis has long remained unknown. Recently, we generated a conditional knock-out (cKO) mouse lacking PHD2 in EPO-producing cells. Thes...

ba0003oc3.2 | Osteoclasts, gastric hormones and HIF | ECTS2014

Activation of the P2Y2 receptor enhances osteoclast function by stimulating the release of ATP, a pro-resorptive extracellular nucleotide

Orriss Isabel , Hajjawi Mark , Arnett Timothy

Extracellular nucleotides, signalling through P2 receptors, play a significant role in bone biology. ATP and ADP act via the P2Y1 or P2Y12 receptors to promote osteoclast formation and activity. Bone cells express the P2Y2 receptor and, in osteoblasts, it plays a role in regulating bone mineralisation. This investigation examined the role of the P2Y2 receptor in osteoclasts. Primary osteoclasts were isolated from the bone ma...

ba0003oc3.3 | Osteoclasts, gastric hormones and HIF | ECTS2014

Thyroid hormones stimulate osteoclastogenesis via TRα-dependent actions in osteoblasts

Logan John G , Bassett JH Duncan , Williams Graham R

Thyrotoxicosis results in osteoporosis, and thyroid hormone (T3) stimulates osteoclastic bone resorption by unknown mechanisms. We previously demonstrated that knockout mice lacking thyroid hormone receptor α (TRα0/0) are euthyroid but have high bone mass, whereas mice lacking TRβ (TRβ−/−) are thyrotoxic and osteoporotic. Tartrate resistant acid phosphatase (TRAcP) staining revealed osteoclast numbers were reduced by 13% (<...

ba0003oc3.4 | Osteoclasts, gastric hormones and HIF | ECTS2014

RANKL enhances TNF-induced osteoclast formation by degrading TRAF3 in osteoclast precursors independent of TRAF6

Yao Zhenqiang , Li Yanyun , Darney Bryant , Boyce Brendan

TNF receptor-associated factors (TRAFs) −6 and −3 regulate RANKL and TNF signaling in osteoclast precursors (OCPs), but they can have opposing effects, and it is not known if their functions are inter-dependent. For example, TRAF6 is required for RANKL/RANK-induced osteoclastogenesis, while TRAF3 limits both RANKL- and TNF-induced osteoclastogenesis through proteasomal degradation of NF-κB-inducing kinase; and inhibition of autophagic degradation of TRAF3 by c...

ba0003oc3.5 | Osteoclasts, gastric hormones and HIF | ECTS2014

Double incretin receptor knock-out (DIRKO) mice present with alterations of trabecular and cortical microarchitectures and bone strength.

Mieczkowska Aleksandra , Mansur Sity , Flatt Peter , Irwin Nigel , Chappard Daniel , Mabilleau Guillaume

Objectives: A role for the gut hormones glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) in controlling bone mass and strength has previously been reported. However, lack of one gut hormone is compensated by an elevated sensitivity to the other in single receptor knockout mice. As such the exact role of GIP and GLP-1 in bone is unclear. The aims of the present study were to assess bone mass and strength in mice with functional deletion of ...

ba0003oc3.6 | Osteoclasts, gastric hormones and HIF | ECTS2014

Skeletal effects of the gastrin receptor antagonist netazepide in H+/K+ATPase beta-subunit deficient mice

Aasarod Kristin Matre , Koldeh Masoud Ramezanzadeh , Mosti Mats Peder , Stunes Astrid Kamilla , Viggaklev Bjorn Ivar , Reseland Janne Elin , Beisvag Vidar , Sandvik Arne Kristian , Skallerud Bjorn Helge , Syversen Unni , Fossmark Reidar

Epidemiological studies suggest that patients using proton pump inhibitors (PPIs) have increased fracture risk. We have previously shown that H+/K+ATPase beta-subunit knockout (KO) mice have reduced BMD, BMC and mechanical bone strength compared to WT. Like users of PPIs, these mice have elevated serum gastrin levels due to high gastric pH. We wanted to study whether elevated gastrin influences bone quality in these mice.Female KO and WT mice aged 6 week...