Searchable abstracts of presentations at key conferences on calcified tissues
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European Calcified Tissue Society Congress 2014

Prague, Czech Republic
17 May 2014 - 20 May 2014

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European Calcified Tissue Society Annual Congress, 17 - 20 May 2014; Prague, Czech Republic

Poster Presentations

Bone biomechanics and quality

ba0003pp16 | Bone biomechanics and quality | ECTS2014

Inhibition of RANKL-mediated bone remodeling decreases bone damage and improves strength in response to fatigue loading

Bonnet Nicolas , Gerbaix Maude , Kostenuik Paul , Ominsky Mike , Ferrari Serge

Antiresorptives consistently improve bone mass and structural strength in normally- and under-loaded bones, but concerns have been raised regarding potential effects on skeletal adaptation to fatigue loading, including damage accumulation and atypical fractures. We thus inhibited or activated osteoclasts with OPG-Fc or RANKL treatment, respectively, and evaluated bone damage and strength after fatigue in the early and later phases of repair. Adult male mice were treated with R...

ba0003pp17 | Bone biomechanics and quality | ECTS2014

Bone fragility and matrix hypermineralization is rescued in homozygous OI Brtl mice mutants

Fratzl-Zelman Nadja , Kozloff Kenneth M , Meganck Jeff , Reich Adi , Roschger Paul , Cabral Wayne , Klaushofer Klaus , Marini Joan

Classical osteogenesis imperfecta (OI) is caused by mutations in the two genes encoding type I collagen. OI is associated with low bone mass and abnormally high bone matrix mineralization. The Brtl/+ OI mouse is a knock-in model caused by a glycine substitution in one COL1A1 allele. Brtl/+ pups display 30% perinatal lethality; survivors have small size and brittle bone. Unexpectedly, homozygous Brtl/Brtl pups, producing only mutant collagen, have normal survival rates...

ba0003pp18 | Bone biomechanics and quality | ECTS2014

Influence of PTH treatment on the bone tissue mechanics of rats with type 2 diabetes mellitus using mechanical tests and finite element modelling

Campbell Graeme , Hamann Christine , Picke Ann-Kristin , Rauner Martina , Huber Gerd , Morlock Michael , Hofbauer Lorenz , Gluer Claus-Christian

Diabetes mellitus results in increased skeletal fragility through reduced bone mineral density and altered collagen structure. How these changes affect bone mechanics at the tissue level remains largely unclear. Anti-osteoporosis medications improve bone mass, but whether they can fully restore tissue strength in diabetic bone, where collagen quality is compromised, has not been fully elucidated. The objective of this study was to determine the effect of type 2 diabetes mellit...

ba0003pp19 | Bone biomechanics and quality | ECTS2014

Scanning acoustic microscopy reveals heterogeneity of mechanical properties due to collagen orientation in mice cortical bone

Blouin Stephane , Puchegger Stephan , Klaushofer Klaus , Roschger Paul , Fratzl Peter

The local mechanical properties of bone are influenced not only by the material chemical composition but also by the spatial arrangement of the component’s e.g. orientation of collagen matrix. However, not much is known about local elastic modulus variations in cortical bone. Our goal was to use acoustic imaging to map elastic properties of murine bone with a several microns resolution. Rodent long bones exhibit a permanent growth with endosteal/periosteal bone formation ...

ba0003pp20 | Bone biomechanics and quality | ECTS2014

Subchondral bone sclerosis in the DMM model of murine OA is not associated with changes in either BMD or nanomechanical properties

Borges Patricia Das Neves , Vincent Tonia L , Oyen Michelle L , Marenzana Massimo

It has long been known that osteoarthritis induces dramatic structural changes in subchondral bone. Studies on patients’ biopsies have also shown that the new sclerotic bone is hypomineralized and has decreased elastic modulus. Due to their amenability for genetic studies, murine models of OA are particularly important for drug target discovery. However, whilst osteosclerosis has been reported also in murine OA models, little is known about the compositional and mechanica...

ba0003pp21 | Bone biomechanics and quality | ECTS2014

Are the high hip fracture rates among norwegian women explained by impaired bone material properties?

Duarte-Sosa Daysi , Vilaplana Laila , Roberto Guerri , Nogues Xavier , Diez-Perez Adolfo , Eriksen Erik Fink

Hip fracture rate in Norway is the highest registered in World, and more than double that of Spanish women. Previous studies were unable to demonstrate significant differences between the two populations with respect to bone mass or calcium metabolism. In order to test, whether the difference in fracture propensity between both populations could be explained by differences in bone material quality we assessed bone material strength using microindentation in 41 Norwegian and 46...

ba0003pp22 | Bone biomechanics and quality | ECTS2014

Bone matrix mineralization is preserved during perimenopausal stage in healthy women

Misof Barbara , Roschger Paul , Recker Robert , Klaushofer Klaus

Menopause is accompanied by a decrease in bone mineral density which can be caused by a reduction in bone volume and/or degree of bone matrix mineralization. Both of them are suggested to contribute to the increased fracture risk in postmenopausal individuals. In the present work, we aimed for information whether a drop in bone matrix mineralization is occurring in the perimenopausal stage of women. For this purpose, we measured the bone mineralization density distribution (BM...

ba0003pp23 | Bone biomechanics and quality | ECTS2014

Influence of estrogen receptor and β-catenin signalling activation on mechanically induced bone formation in ovariectomized mice

Nemitz Claudia , Jakob Franz , Ignatius Anita , Liedert Astrid

Dysfunctions of Wnt/β-catenin and estrogen receptor signalling resulted in impaired mechanotransduction and bone loss as in osteoporosis. Previous studies demonstrated the interaction of these pathways in mechanotransduction in vitro and in vivo. In this study, the influence of the activation of estrogen receptor and β-catenin signalling on mechanically induced bone formation was investigated in ovariectomized mice. 12-week-old mice were ovariectomi...

ba0003pp24 | Bone biomechanics and quality | ECTS2014

Beneficial effects of a GIP mimetic on bone material properties

Mabilleau Guillaume , Mieczkowska Aleksandra , Irwin Nigel , Flatt Peter , Chappard Daniel

Objectives: A role for glucose-dependent insulinotropic polypeptide (GIP) in controlling bone mass and strength has previously been reported. However, the rapid degradation of GIP in the bloodstream by the dipeptidyl peptidase-4 enzyme precludes therapeutic use. To circumvent this problem, a series of N-terminally modified GIP agonists have been developed. The aim of the present study was to investigate the effects of 28-day treatment with N-AcGIP on bone microarchitecture and...

ba0003pp25 | Bone biomechanics and quality | ECTS2014

Normal bone matrix mineralization in patients with chronic obstructive pulmonary disease

Misof Barbara , Roschger Paul , Jorgetti Vanda , Klaushofer Klaus , Dempster David , Kulak Carolina

Chronic obstructive pulmonary disease (COPD) has been found associated with low areal bone mineral density and an increase in fracture rate. Previous histomorphometric findings revealed abnormally low cancellous bone volume and thin cortices. In the present work, we studied the same transiliac bone biopsy samples from n=19 COPD patients for cancellous (Cn.) and cortical (Ct.) bone mineralization density distribution (BMDD) based on quantitative backscatter electron im...

ba0003pp26 | Bone biomechanics and quality | ECTS2014

Structural analysis of tooth and jawbone in a type 2 diabetes mouse model

Pabisch Silvia , Yamaguchi Tsuguno , Koike Yasushi , Egashira Kenji , Kataoka Shinsuke , Wagermaier Wolfgang , Weinkamer Richard , Murakoshi Michiaki , Fratzl Peter

In type 2 diabetes mellitus (T2DM) patients, an increased fracture risk is observed, although the bone mineral density is even higher than in non-diabetic patients, which raises the question of the quality of the organic and inorganic matrix in bone1,2. T2DM is also known to favor inflammation of the gingiva and paradontosis in general. However, little is known about the mineral nano-architecture in the mandible and about the possible influence of diabetes. Using sy...

ba0003pp27 | Bone biomechanics and quality | ECTS2014

Long term treatment with odanacatib maintains normal trabecular biomechanical properties in ovariectomized adult monkeys as demonstrated by micro-CT based finite element analysis of the vertebral cores

Cabal Antonio , Jayakar Richa Y , Zhang Jingru , Sardesai Swanand , Williams Donald S , Duong Le T

The cathepsin K inhibitor odanacatib (ODN) is a bone formation-sparing inhibitor of osteoclastic resorption activity. This drug is currently under development for the treatment of postmenopausal osteoporosis. To support the bone safety profile of ODN, we evaluated the effects of ODN on trabecular bone hard tissue properties in the estrogen-deficient model of the ovariectomized (OVX) rhesus monkeys. Animals (n=16/group, age 11–22 years) were treated immediately af...

ba0003pp28 | Bone biomechanics and quality | ECTS2014

Dietary creatine influence on bone metabolism in rats

Zivny Pavel , Zivna Helena , Svejkovska Klara , Fekete Sona , Holecek Milan

Objective: We investigated the effect of creatine administered in the diet for 10 weeks on bone turnover and bone quality in male Wistar rats.Methods: Rats were divided (after approval of Ethical committee 9814/2008–30 and 30793/2010–30) into four groups, ten animals in each group. G1 SLD-A group were fed a standard laboratory diet (SLD). G2 SLD-B group were fed SLD and 12 h before rats were sacrificed diet was removed. G3...

ba0003pp29 | Bone biomechanics and quality | ECTS2014

Systemic treatment with strontium ranelate does not influence the healing of femoral mid-shaft defects in rats

Vegger Jens Bay , Bruel Annemarie , Thomsen Jesper Skovhus

The objective of the study was to investigate whether the anti-resorptive and bone-forming dual effect of Strontium Ranelate (SrR) influences the healing of a non-critical sized femoral mid-shaft bone defect.Sixty 16-weeks-old female Wistar rats were randomized into four groups. Cylindrical defects with a diameter of 2 mm was drilled through the anterior cortex of both right and left femoral mid-shafts. Two groups were treated with SrR (900 mg/kg/day) mi...

ba0003pp30 | Bone biomechanics and quality | ECTS2014

Strength training is capable of stimulating transcription factors Runx2 and osterix and ensure better bone quality in wistar rats during aging

Stringhetta-Garcia Camila Tami , Ervolino Edilson , Rossi Ana Claudia , Louzada Mario Jefferson Quirino , de Mello Wagner Garcez , Menegati Dornelles Rita Cassia

Osteoporosis is a multifactorial disease that represents an increase public health problem, given the impact on functional independence and quality of life. Among the favoring factors to the imbalance in bone cell activity, the hypoestrogenism is primordial. Strength training (ST) proves to be effective because of its ability to stimulate estrogen receptor independent of ligand. In this study, we analyzed the action of ST on bone quality of rats during the aging. For this stud...

ba0003pp31 | Bone biomechanics and quality | ECTS2014

Excessive intake of soy milk affects bone development

Martins Oliveira Bruna Rezende Silva , de Carvalho Apolinario Coelho Juliana , Medeiros Ronaldo Anderson , Louzada Mario Jefferson Quirino

The purpose of this study was to analyze the influence of diet supplemented with hydrosoluble soy extract – HSE in bone mass of male and healthy Wistar rats. Sixteen (16) 30 days old animals were kept in controlled conditions with light/dark cicle and fed with commercial food and filtered water. Randomized into two groups: control group received 500 ml of water and 300 g of food, daily; supplemented group S, in addition of water and food, they also received 500 ml of soy ...

ba0003pp32 | Bone biomechanics and quality | ECTS2014

Dietary arginine influence on bone metabolism in rats

Zivna Helena , Svejkovska Klara , Fekete Sona , Zivny Pavel , Holecek Milan

Objective: We investigated the effect of arginine administered for 10 weeks in diet on bone metabolism in male Wistar rats.Methods: Rats were divided (after approval of Ethical Committee 9814/2008-30 and 30793/2010-30) into four groups, ten animals in each group: G1 SLD-S group were fed a standard laboratory diet (SLD). G2 SLD-H group were fed SLD and 12 h before rats were sacrificed diet was removed. G3 ARG-S group was fed wit...

ba0003pp33 | Bone biomechanics and quality | ECTS2014

Relationships between morphometric, densitometric and mechanical properties of tarsometatarsal bone in ostriches (Struthio camelus)

Tatara Marcin , Charuta Anna , Krupski Witold , Horbanczuk Jaroslaw , Luszczewska-Sierakowska Iwona , Chmielowiec Krzysztof

The aim of the study was to evaluate relationships between morphometric, densitometric and mechanical properties of tarsometatarsus in ostriches (Struthio camelus). Males (n=9) and females (n=15) were kept to slaughter age of 14 months of life to obtain left tarsometatarsus. Using computed tomography technique (Somatom Emotion, Siemens), volumetric bone mineral density (vBMD) of the trabecular (Td) and cortical bone (Cd) were measured. Areal BMD and ...

ba0003pp34 | Bone biomechanics and quality | ECTS2014

The surface and the volume trace element compound of the of biological bone apatite

Gusak Eugeniya , Vladimir Kuznetsov , Alexandr Kochnko , Elena Gordienko , Sergii Danilchenko , Maksym Pogorielov

The bioapatite crystals have a multilayer hydrate shell, which contains impurity ions such as magnesium, sodium, potassium and it is not clear the qualitative and quantitative characteristics of noapatite entourage. The major component noapatite environment in bone is water and we studied the surface of nanocrystals from trabecular bone in healthy rats and during the water deficiency.In experiment we use adult laboratory rats (8 months age), remove the c...

ba0003pp35 | Bone biomechanics and quality | ECTS2014

Strength features of humerus in rats with defects in tibiae after 60-day administration of sodium benzoate

Lukyantseva Galina , Luzin Vladyslav

Aim: Investigate strength features of humerus in rats with defects in tibiae after 60-day per os administration of sodium benzoate (SB).Materials and methods: The experiment involved 210 rats with initial body weight of 200–210 g. The animals were separated into six groups as follows: Group 1 comprised animals that received per os 1 ml of 0.9% solution of sodium chloride daily (C), Group 2 (D) comprised animals with plain defects o...

ba0003pp36 | Bone biomechanics and quality | ECTS2014

Comparing two types of bovine bone composition, structure and hardness based on their ecological effect and nutrition

Fard Negar Gerami , Bahrololoom Mohammad , Khajouei Fariba

Two kinds of apatite powder were prepared separately from bovine bones originated from different geographical and ecological areas with different climates, in order to characterize and compare their components, hardness and structures by burning them and heat treating the bone ashes at 850 °C in an air furnace. The black ashes were converted to white and yellow–white powders. X-ray diffraction (XRD) analysis indicate that the white powder was carbonate–hydr...

ba0003pp37 | Bone biomechanics and quality | ECTS2014

Bone matrix mineralization after sclerostin antibody treatment in a mouse model of osteogenesis imperfecta

Roschger Andreas , Roschger Paul , Kneissel Michaela , Rauch Frank

Children with osteogenesis imperfecta (OI) still suffer from frequent fractures, despite bisphosphonate treatment. Thus new therapeutic approaches are needed. Sclerostin is a protein that is thought to inhibit bone formation. Treatment with sclerostin antibodies (SclAB) increases bone mass in animal models and in clinical trials and may be a rational therapy for OI as well.Transgenic (TgOI) Col1a1Jrt/+ mice were gene...