Bone Abstracts

Osteosarcoma is the main malignant primary bone tumor in children and adolescents for whom the prognosis remains poor, especially when metastases are present at diagnosis (survival rate drops to 20% when lung metastases were detected). Because TGF-β has been shown to promote metastases in many solid tumors, we investigated the effects of inhibition of the TGF-β/Smad cascade on osteosarcoma behavior. To this end, two independent procedures, a pharmacological approach ...

Introduction and objective: Despite recent improvements in therapeutic management of osteosarcoma, ongoing challenges in improving the response to chemotherapy warrants the development of new strategies to improve overall patient survival. Among new therapeutic approaches, heat shock protein (HSP) 90 is a molecular chaperone involved in the maturation and stability of various oncogenic proteins leading to tumor cells survival. In this study, we assessed the in vitro a...

Tumour growth and metastatic dissemination are significantly reduced in mice bearing an inactivation of the macrophage-colony stimulating factor (M-CSF) gene, due to angiogenesis impairment. In fact, M-CSF directly induces angiogenesis by increasing vascular endothelial growth factor (VEGF) production. Interleukin 34 (IL34), the M-CSF’s ‘twin’ cytokine, was characterized as a new cytokine promoting the growth, survival and differentiation of the myeloid lineage....

Introduction: Umbilical cord blood (UCB) is increasingly used in hematopoietic stem cell (HSC) transplantations; however, the low cell numbers are still remaining as a limiting factor for proper engraftment. Osteoblasts are major constituents of HSC niche and play important roles in regulating HSC self-renewal and differentiation. Recently, extracellular vesicles (EVs) have been implicated in stem cell fate regulation via horizontal transfer of proteins and nucleic acids betwe...

Tumour necrosis factor receptor associated factors (TRAFs) play a key role in signal transduction in mammalian cells. Several members of the TRAF family have been identified but only TRAF2 and TRAF6 are implicated in the regulation of osteoclastogenesis. Here we studied the role of TRAF2 and TRAF6 in breast cancer induced bone cell activity and osteolysis. We observed that TRAF2, but not TRAF6, is highly expressed in the human MDA-MB-231 (MDA-231) bone-seeking breast cancer ce...

Giant cell tumour of bone (GCTb) is an osteolytic neoplasia with tendency to local recurrence (10–25%), while metastases or malignant transformation are described in 1–4% of cases.It is composed by three distinct populations that cross-talk each other generating unbalance in bone remodeling and activation of NF-kB signaling pathway.To identify new candidate biological markers useful for improving clinical management of GC...

Postmenopausal women with early stage hormone-receptor-positive breast cancer (EBC) are standardly treated with aromatase inhibitors (AIs). However, one side-effect of AIs treatment is a decrease in bone mineral density (BMD) and an increased risk of fracture. The objectives of this study were to examine: i) changes in bone formation (N-terminal propeptide of type I procollagen; PINP) and bone resorption (cross-linked C-telopeptides of bone type I collagen; CTX) markers, as we...

Objectives: Most osteolytic tumors are in fact mixed and contain an osteoblastic component associated with the predominant osteolytic areas. This metaplastic woven bone can be evidenced on X-rays as blurred white areas but is always evidenced by histological analysis even in the absence of radiological expression. Metaplastic bone formation reflects the activation of new osteoblasts, the stimulation of the dormant lining cells due to the release of growth factors buried in the...

Introduction: Granulocyte colony-stimulating factor (G-CSF) is widely used to mobilize peripheral blood stem cells (PBSC) and enable PBSC collection by apheresis. Although bone pain is a common adverse event following G-CSF treatment, little is known on its effect on bone metabolism.Methods: Markers of bone turnover (OC, osteocalcin, β-CTx, bALP, C-terminal telopeptide of type I collagen, bone specific alkaline phosphatase, TRAP, tartrate resistant ...

Paget’s disease of bone is a condition where the process of bone remodeling is disrupted; Its primary event is an increased bone resorption followed by a subsequent reactive bone formation. The disease is most common in central Europe, the United Kingdom, Australia, and New Zealand. It is also found, though with lower incidence, in southern Europe, Scandinavia, and the United States. It is extremely rare in East Asian countries, especially in Korea, Japan, China, the Midd...

The vicious cycle established between bone associated tumors and bone resorption is the central problem with therapeutic strategies against primary bone tumors and bone metastasis. The bromodomain and extra-terminal domain (BET) protein family is an important class of ‘histone reading protein’ capable to recognize the N-acetylation of lysine residues on histone tails. BET bromain proteins have recently been described as regulators of MYC expression in various tumors....