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Bone Abstracts (2014) 3 PP12 | DOI: 10.1530/boneabs.3.PP12

Arthritis and other joint diseases: translational and clinical

Cartilage oligomeric matrix protein assay on the IDS-iSYS automated system

Pilar Lorenzo1, Jenny Manolopoulou2 & Zoltan Seres2

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1Section of Molecular Skeletal Biology, Clinical Sciences Lund, Lund University, Lund, Sweden; 2Immunodiagnostic Systems (IDS) Ltd, Boldon, UK.


Diseases affecting the musculoskeletal system are a major cost burden to society. Articular cartilage loss or damage in these diseases is detected by radiography and measuring decreases in joint space width (JSW). The early stages of the disease may remain latent and asymptomatic for many years. This forms the rationale for applying molecular marker technology (biomarkers) to identify patients prone to joint diseases. One such biomarker is cartilage oligomeric matrix protein (COMP, thrombospondin five). COMP can be used to monitor the progress of cartilage degradation in inflammatory joint diseases such as rheumatoid arthritis (RA) and osteoarthritis (OA). Clinical studies have shown that measurements of serum COMP can be used a valuable tool for identifying patients at high risk from rapid joint destruction and for monitoring treatment efficacy.

We report the results of an automated COMP assay on the IDS-iSYS.The COMP assay is based on the direct sandwich technique in which two specific monoclonal antibodies are directed against separate antigenic determinants on the COMP molecule.

Below are the preliminary analytical performance of the IDS-iSYS COMP assay.

PerformancesResults
Inter-assay precisionFive samples tested over 12 days gave %CV’s between 4 and 9% on dose at concentrations between 10 and 46 μg/ml.
SensitivityLimit of blank: 0.011 μg/ml; limit of detection: 0.027 μg/ml; limit of quantitation: <0.0625 μg/ml
LinearityVariation being within ±10%
RecoveryEight tests gave a range of 102–112%
High dose hook effectNo hook effect observed up to 100 μg/ml
Method comparison against AnaMar COMP kitiSYS COMP =1.425×(AnaMar) −0.36 μg/ml R2=0.64

Volume 3

European Calcified Tissue Society Congress 2014

Prague, Czech Republic
17 May 2014 - 20 May 2014

European Calcified Tissue Society 

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