Collagen barrier membranes are used in guided tissue regeneration to support bone healing and periodontal regeneration. Pharmacological inhibitors of prolyl-hydroxylases (PHD) can support hard and soft tissue regeneration and are therefore a promising tool for periodontal therapy. Here we evaluate the release kinetic of the PHD inhibitors dimethyloxaloylglycine and L-mimosine from the collagen barrier membranes.
The PHD inhibitors were lyophilized onto collagen barrier membranes. Morphology of the membranes was assessed with scanning electron microscopy. The release kinetic was assessed in a bioassays with gingival and periodontal ligament fibroblasts.
We found that the membranes treated with PHD inhibitors stimulated VEGF production. Assessment of the release kinetic based on VEGF production, metabolic activity and proliferation showed that supernatants obtained from the collagen barrier membranes in the first hours had a sufficient level of PHD inhibitors to induce a cellular response. Morphology of the membranes was unchanged by the treatment with PHD inhibitors.
In conclusion collagen barrier membranes supplemented with PHD inhibitors can provoke a pro-angiogenic response in vitro. The membranes release PHD inhibitors in a burst like kinetic within the first hours. These findings provide the basis for preclinical studies to evaluate the regenerative capacity of PHD inhibitors in periodontal surgery.
17 May 2014 - 20 May 2014