Searchable abstracts of presentations at key conferences on calcified tissues
Bone Abstracts (2014) 3 PP143 | DOI: 10.1530/boneabs.3.PP143

ECTS2014 Poster Presentations Cell biology: osteoblasts and bone formation (48 abstracts)

Utilization of L-mimosine in pulp regeneration: lessons from cell culture and tooth slice organ cultures

Heinz-Dieter Müller 1, , Katharina Trimmel 6 , Barbara Cvikl 2, , Reinhard Gruber 3, & Hermann Agis 2

1Department of Prosthodontic, Medical University of Vienna, Vienna, Austria; 2Department of Conservative Dentistry and Periodontology, Medical University of Vienna, Vienna, Austria; 3Department of Oral Surgery, Medical University of Vienna, Vienna, Austria; 4Austrian Cluster for Tissue Regeneration, Vienna, Austria; 5Cell Culture Laboratory of Dental School of Medicine, University of Bern, Bern, Switzerland; 6Bernhard Gottlieb University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.

After trauma or carious lesion dental pulp healing is difficult to predict. In addition systemic diseases like diabetes mellitus can impair the regenerative capacity. New regenerative strategies target prolyl hydroxylase (PHD) by pharmacological inhibitors to stimulate hard and soft tissue healing. PHD inhibitors such as L-mimosine (L-MIM) induce vascular endothelial growth factor (VEGF) production by promoting angiogenesis. However, it is unclear if L-MIM is a feasible tool to stimulate pulp regeneration.

In this study we investigated the response of the dental pulp to L-MIM in monolayer cultures based on viability, proliferation and VEGF production utilizing MTT-tests, 3[H]thymidine incorporation assays, and immunoassays respectively. In addition viability and VEGF production were assessed in tooth slice organ models. To mimic the diabetic milieu, cultures were performed in the presence of advanced glycolysed end-products (AGE).

We found that L-MIM at non-toxic concentration enhances VEGF production under basal conditions in monolayer cultures. This enhanced pro-angiogenic capacity was paralleled by an increase in VEGF production in the tooth slice model. L-MIM elevated the VEGF levels also in monolayer and tooth slice organ cultures performed in the presence of AGE.

Overall these results indicate that the dental pulp responds to L-MIM under basal and under diabetic conditions. Further studies will show if this pro-angiogenetic response to L-MIM found in vitro translates to enhanced pulp regeneration.

Volume 3

European Calcified Tissue Society Congress 2014

Prague, Czech Republic
17 May 2014 - 20 May 2014

European Calcified Tissue Society 

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