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Bone Abstracts (2014) 3 PP43 | DOI: 10.1530/boneabs.3.PP43

Bone development/growth and fracture repair

Subchronic co-administration to cadmium, diazinon and selenium causes apparent symptoms of osteoporosis in adult male rats

Radoslav Omelka1, Hana Chovancova1, Ivana Bobonova1, Grzegorz Formicki2, Robert Toman3 & Monika Martiniakova1

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1Constantine the Philosopher University in Nitra, Nitra, Slovakia; 2Pedagogical University, Cracow, Poland; 3Slovak University of Agriculture, Nitra, Slovakia.


Osteoporosis, as a global public health concern, may be induced by various hazardous substances such as cadmium (Cd) and pesticide diazinon (DZN). Selenium (Se), an essential trace element, can adversely modify bone structure in higher concentrations. The current study was designed to investigate structural changes in femoral bone of adult male rats after simultaneous co-administration to Cd, DZN, and Se. A total of 20 male Wistar rats were randomly divided into two experimental groups. In the first group, young males (1-month-old) were exposed to a combination of 30 mg CdCl2/L, 40 mg DZN/L, and 5 mg Na2SeO3/L in their drinking water, for 90 days. Ten 1-month-old males without toxicant application served as a control group. At the end of experiment, macroscopic and microscopic analyses of femora were performed. Our results showed no significant differences in body weight, femoral weight and femoral length between the two groups. On the other hand, cortical bone thickness was considerable decreased in rats simultaneously administered to Cd, DZN, and Se (P<0.05). Additionally, a smaller number of primary and secondary osteons was identified in these rats. Apparent symptoms of osteoporosis such as resorption lacunae and osteoporotic fractures were observed in Cd-DZN-Se-intoxicated rats. Histomorphometric evaluation revealed that area, perimeter, maximum, and minimum diameters of primary osteons’ vascular canals, Haversian canals, and secondary osteons were significantly decreased (P<0.05) in rats exposed to Cd, DZN, and Se. Our study demonstrates that subchronic co-administration to Cd, DZN, and Se harmfully affects cortical bone thickness, vascularization, and induces evident clinical manifestations of osteoporosis in male rats.

Volume 3

European Calcified Tissue Society Congress 2014

Prague, Czech Republic
17 May 2014 - 20 May 2014

European Calcified Tissue Society 

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