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Bone Abstracts (2014) 3 PP66 | DOI: 10.1530/boneabs.3.PP66

Bone development/growth and fracture repair

Interrelationships between body weight, bone-specific alkaline phosphatase BAP, IGF1 and morphometric, densitometric and mechanical properties of deciduous mandibular teeth properties in Polish Merino Sheep

Witold Krupski1, Anna Szabelska2, Marcin Tatara1,3, Iwona Luszczewska-Sierakowska4, Barbara Tymczyna5, Jaroslaw Bienias6 & Monika Ostapiuk6

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1II Department of Radiology, Medical University in Lublin, Lublin, Poland; 2Department of Prosthetic Dentistry, Medical University in Lublin, Lublin, Poland; 3Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland; 4Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland; 5Department of Conservative Dentistry and Endodontics, Medical University in Lublin, Lublin, Poland; 6Department of Materials Engineering, Lublin University of Technology, Lublin, Poland.


The aim of this study was to determine interrelationships between final body weight, bone-specific alkaline phosphatase (BAP), IGF1 and morphological, densitometric and mechanical properties of deciduous mandibular teeth isolated from seven healthy Polish Merino rams. Four incisors (I1–I4) and second premolar (P2) were obtained from 5-month-old sheep. Teeth were cleaned, weighed, and their length was measured. Mean volumetric tooth mineral density (MvTMD) and total tooth volume (Tvol) were determined using quantitative computed tomography (QCT) and SOMATOM EMOTION SIEMENS apparatus (Siemens, Erlangen, Germany) equipped with Somaris/5 VB10B software and Volume Evaluation application package. For Tvol and MvTMD determinations, volume-of-interest was defined between minimum and maximum density of the investigated samples at 0 and 3071 Hounsfield units (HU), respectively. Micro computed tomography (micro CT) and SkyScan 1174 apparatus (SkyScan n.v., Kontich, Belgium) were used to measure total enamel volume (Evol), volumetric enamel mineral density (vEMD), total dentine volume (Dvol) and volumetric dentine mineral density (vDMD). The volume-of-interest was defined between minimum and maximum density of the investigated samples at –1000 and 3071 HU. To determine mechanical properties, all incisors were subjected to a three-point bending test and maximum elastic strength (Wy) and ultimate force (Wf) were determined. In compression test Wf of P2 was determined (Zwick/Roell Z010 apparatus; Zwick, Ulm, Germany). BAP and IGF1 were determined in serum of 28- and 150-day-old sheep using immunoenzymometric assays (Ostease BAP and IGF1 ELISA, Immunodiagnostic Systems Ltd, Boldon, Tyne & Wear, UK). Pearson’s correlation coefficient (r) between final body weight, BAP, IGF1 and morphological, densitometric and mechanical properties of teeth was determined. Final body weight was positively correlated with Tvol and Dvol of I1, I4 and P2 (r>0.88; P<0.05). IGF1 in 28-day-old sheep was negatively correlated with MvBMD of I1, I2, I3, I4 and P2 (r<−0.82; P<0.05). IGF1 in 28-day-old sheep was positively correlated with Wf of P2 (r=0.84; P<0.05). IGF1 in 150-day-old sheep was negatively correlated with vDMD of I2, and Tvol and Dvol of I4 (r<−0.86; P<0.05). BAP in 150-day-old sheep was negatively correlated with Wy and Wf of I2 and MvBMD of P2 (r<−0.76; P<0.05). In conclusion, this study revealed that body weight of sheep is associated with morphological properties of teeth. Interrelationships of IGF1 and BAP concentration in serum with morphological, densitometric and mechanical properties were proven.

Volume 3

European Calcified Tissue Society Congress 2014

Prague, Czech Republic
17 May 2014 - 20 May 2014

European Calcified Tissue Society 

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