Searchable abstracts of presentations at key conferences on calcified tissues
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43rd Annual European Calcified Tissue Society Congress

Rome, Italy
14 May 2016 - 17 May 2016

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ba0005cabs.op1.1 | Oral Poster Talks | ECTS2016

Osteoblastic and osteolytic bone metastases induce divergent angiogenic responses

Hensel Janine , Riether Carsten , Wetterwald Antoinette , Temanni Ramzi , Kruithof-de Julio Marianna , van der Pluijm Gabri , Thalmann George N. , Cecchini Marco G.

Cancer cell growth is dependent on the microenvironmental support. Prostate and mammary cancer (PCa and MCa) cells preferentially metastasize to bone, where they induce either an osteoblastic or osteolytic response. These opposite stromal responses suggest that different types of cancers adopt distinct strategies to hijack the bone marrow/bone stroma for their growth support. However, the molecular cues underlying these divergent responses are largely elusive.<p class="abs...

ba0005cabs.op1.2 | Oral Poster Talks | ECTS2016

Roundabout receptors: new actors in bone metastatic niche formation

Clement-Demange Lise , Le Pape Francois , Eckel Benedicte , Geraci Sandra , Diaz-Latoud Chantal , Clezardin Philippe

Roundabout receptors are crucial regulators of vessels and axons growth during development. A comparative transcriptomic study between the MDA-MB-231 breast cancer cell line and its osteotropic subpopulation, MDA-B02 cells, has shown an overexpression of ROBO4 receptor. Interestingly, a high ROBO4 expression in primary tumour from patients with breast cancer correlates with poor prognosis and increased risk of bone metastasis relapse. Inoculation of ROBO4 depleted cells in the...

ba0005cabs.op1.3 | Oral Poster Talks | ECTS2016

Cripto/Grp78 drive the metastatic phenotype in human osteotropic prostate cancer

Zoni Eugenio , Chen Lanpeng , Granchi Zoraide , Karkampouna Sofia , La Manna Federico , Verhoef Ester , Pelger Rob , Snaar-Jagalska Ewa , van Leenders Geert , Beimers Lijkele , Kloen Peter , Gray Peter , van der Pluijm Gabri , Kruithof-de Julio Marianna

Prostate cancer is the most prevalent cancer in men and metastatic spread to bone is detected in up to 80% of patients with advanced disease at autopsy. PCa can progress from treatable androgen-dependent stage to castration-resistant stage with distant metastases for which novel therapeutic targets and strategies are urgently needed. Here we identify the cell surface/secreted oncoprotein Cripto as a potential target for the diagnosis and treatment of metastatic prostate cancer...

ba0005cabs.op1.4 | Oral Poster Talks | ECTS2016

p62-ZZ domain signaling inhibition prevents MM cell-induced epigenetic repression at the Runx2 promoter and rescues osteoblast differentiation

Silbermann Rebecca , Adamik Juraj , Zhou Dan , Xie Xian-Qun , Kurihara Noriyoshi , Galson Deborah L , Roodman G David

Multiple myeloma (MM) bone disease is characterized by lytic bone lesions that contribute to patient morbidity and mortality after patients are in complete remission. The mechanisms mediating this long-term osteoblast (OB) suppression are poorly understood. We hypothesized that MM cells induce epigenetic changes at the Runx2 promoter in preOB bone marrow stromal cells (BMSC). We demonstrated that Gfi1, a transcriptional repressor of Runx2 that is induced in B...

ba0005cabs.op2.1 | Oral Poster Talks | ECTS2016

Antagonizing Mir-218 prevents breast cancer-induced osteolytic disease

Taipaleenmaki Hanna , van Wijnen Andre J , Stein Janet L , Hesse Eric , Stein Gary S , Lian Jane B

Signaling pathways crucial in bone development, including Wnt, are also upregulated in breast cancer cells to promote tumor growth in the skeleton, a process known as osteomimicry. Thus, we hypothesized that bone metastatic tumor cells also aberrantly express osteogenic miRNAs to support osteomimetic properties. We have previously shown that miR-218 is highly expressed in osteoblasts and promotes osteogenic differentiation. Interestingly, expression analysis revealed a signifi...

ba0005cabs.op2.2 | Oral Poster Talks | ECTS2016

Mesenchymal stromal cells promote osteosarcoma stemness and migratory potential via IL-6 secretion

Cortini Margherita , Avnet Sofia , Bonuccelli Gloria , Baldini Nicola

Osteosarcoma (OS) is an aggressive malignancy characterized by a high relapse rate despite multiagent chemotherapy. As for other cancers, OS microenvironment contributes to tumor initiation, growth, and metastasis. We consider bone marrow mesenchymal stromal cells (MSC) as a major nontumor component of OS microenvironment, and have previously found that the interaction between MSC and tumor cells is bidirectional, i.e. tumor cells can modulate their peripheral environment that...

ba0005cabs.op3.1 | Oral Poster Talks | ECTS2016

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ba0005cabs.op3.2 | Oral Poster Talks | ECTS2016

Lysyl oxidase promotes survival and outgrowth of colon cancer cells in the bone marrow, enabling bone metastasis formation

Reynaud Caroline , Ferreras Laura , Brevet Marie , Clezardin Philippe

Lysyl oxidase (LOX) catalyzes the cross-linking of collagens and elastin in the extracellular matrix, thereby regulating the tensile strength of many tissues, such as in bone. In cancer, LOX plays a critical role in facilitating tumor growth and metastasis formation in soft tissues. In this study, we first showed by immunohistochemistry using patientsÂ’ tumor specimens, that LOX was expressed in the desmoplastic tumor stroma of pairs of colorectal carcinomas and their matc...