Searchable abstracts of presentations at key conferences on calcified tissues
Bone Abstracts (2016) 5 P266 | DOI: 10.1530/boneabs.5.P266

ECTS2016 Poster Presentations Muscle, physical activity and bone (14 abstracts)

Inhibition of Cyclooxygenase 2 pathway modulates collagen synthesis of cytokine-stimulated fibroblasts from Ligamentum flavum

Young-Mi Kang 1, , Ji-Hye Kim 1 , Jae-Ho Yang 1 , Hwan-Mo Lee 1 & Seong-Hwan Moon 1,


1College of Medicine, Yonsei University, Seoul, Republic of Korea; 2Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Republic of Korea.


Introduction: Hypertrophy of ligamentum flavum (LF) induces narrowing of spinal canal which develops neurogenic claudication. Several mechanisms of LF hypertrophy have been suggested. Among them inflammatory cytokine play a crucial role in LF hypertrophy by increasing collagen synthesis. Cyclooxygenase-2 (COX-2) pathway shares inflammatory reaction from infection and arthritis. Selective COX-2 inhibitor (COX-2si) might modulates collagen synthesis via suppressing inflammatory pathway. Hence, the current study examined the effect of COX-2si in collagen synthesis of inflammatory cytokine induced LF cells.

Materials and methods: Fibroblast and LF cells were harvested and cultured. Inflammatory cytokines (IL-1, TNF-s) were utilized to stimulate collagen synthesis of LF cells. Then COX-2si was administered to stimulated fibroblasts and LF cells. Collagen synthesis, RT-PCR for COX-2 and various collagens, were performed.

Results: Fibroblasts and LF cells stimulated by inflammatory cytokines showed increase in collagen synthesis in translational and transcriptional level. Stimulated fibroblasts and LF cells with COX-2si demonstrated down-regulation of COX-2, various collagens mRNA, and finally collagen synthesis.

Summary and conclusion: In stimulated fibroblasts and LF cells, COX-2si provided therapeutic mechanism in reducing collagen synthesis. Hence COX-2si might be useful in preventing LF hypertrophy, which opens new therapeutic, preventive measures in symptomatic lumbar spinal stenosis.

Keywords: COX-2, ligamentum flavum, fibroblast, spinal stenosi

Volume 5

43rd Annual European Calcified Tissue Society Congress

Rome, Italy
14 May 2016 - 17 May 2016

European Calcified Tissue Society 

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