ECTS2016 Poster Presentations Cell biology: osteoblasts and bone formation (36 abstracts)
L-Carnitine facilitates mitochondrial activity and osteogenic differentiation in human primary osteoblast culture
Fernanda Vacante 4 , Anna Montesano 2 , Alice Spinello 3 , Simona Bolamperti 3 , Pamela Senesi 1 , Ileana Terruzzi 4 , Livio Luzi 1, , Isabella Villa 3 & Alessandro Rubinacci 3
1Metabolism Resesarch Centre San Donato Hospital Scientific Institute, Milano, Italy; 2Department of Biomedical Sciences for Health, University of Milano, Milano, Italy; 3Bone Metabolism Unit IRCCS San Raffaele Scientific Institute, Milano, Italy; 4Metabolism Nutrigenomics & Cellular Differentiation IRCCS San Raffaele Scientific Institute, Milano, Italy.
Osteoblasts have a high rate of energy consumption during bone formation and bone protein synthesis. Therefore, it is likely that decreased energy production that accompanies aging, could contribute to reduced osteoblast activity, a critical feature of senile osteoporosis, and that this reduction might be counteracted by favoring energy availability. Cells of the osteoblastic lineage generate 40–80% of the requested energy through fatty acid degradation, thus the modulation of lipid oxidation could be involved in the availability of energy required for protein synthesis. L-Carnitine (LC) promotes energy availability, as it is an essential cofactor for the transport of fatty acid into the mitochondrial matrix where β-oxidation occurs. Based on these evidences, we studied the possible stimulatory effects of L-Carnitine on cells of the osteogenic lineage. For this purpose we treated human osteoblasts (hOb) derived from trabecular bone waste material of orthopaedic surgery (Ethics Committee approved), with 5 mM LC at different times,3,6 and 24 h.
After 24 h treatment LC induced an increase in mitochondrial activity, as shown by Mito Tracker Dye assay. This effect was accompanied by a significant increase of SOD2 protein levels, which plays an important role in neutralizing oxidative stress, and an increase in Calcium-Calmodulin Kinase II protein (CaMKII). Since CaMKII is able to favour synthesis and transcriptional activity of osterix (OSX), we evaluated the effect of LC on osteogenesis-related genes. LC increased significantly (P<0.01) OSX and bone sialo-protein mRNA expression. Moreover, LC stimulated osteopontin mRNA and protein expression. Altogether these results suggest that LC, exerts a stimulatory effect on bone by favouring osteoblast differentiation. Considering its safety profile, LC could be a useful supplement to support bone health in the elderly.
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Bone Abstracts
ISSN 2052-1219 (online)
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