ECTS2016 Poster Presentations Bone development/growth and fracture repair (35 abstracts)
In this study, we evaluated the effect of the association of a bioactive glass-ceramic scaffold (Biosca) with mesenchymal stem cells derived from either bone marrow (BM-MSCs) or adipose tissue (AT-MSCs) on bone formation in calvarial defects of osteoporotic rats. Wistar rats were submitted to bilateral ovariectomy (OVX) or only to the surgical stress (Sham), under approval of the Committee of Ethics in Animal Research. After 5 months, 5-mm unilateral calvarial defect was created and filled with Biosca combined with BM-MSCs or AT-MSCs. Biosca without cells and empty defects were used as controls. After 4 weeks, the calvariae were harvested, fixed and analysed by microtomography to evaluate bone volume (BV mm3), percentage of bone volume (%BV) and bone surface (BS mm2). Data were compared using ANOVA test (P≤0.05). For OVX and Sham rats, BV was, respectively, 1.91±0.64 and 1.30±0.70 in Biosca with BM-MSCs, 2.13±0.77 and 1.50±0.75 in Biosca with AT-MSCs, 2.88±1.14 and 2.49±1.12 in Biosca, and 0.40±0.55 and 0.47±0.03 in empty defects. The %BV was, respectively, 6.45±2.18 and 4.40±2.36 in Biosca with BM-MSCs, 7.22±2.62 and 5.07±2.53 in Biosca with AT-MSCs, 9.57±3.87 and 8.40±3.8 in Biosca, and 1.37±1.85 and 1.61±0.12 in empty defects. The BS was, respectively, 297.29±84.13 and 210.59±116.29 in Biosca with BM-MSCs, 310.75±105.46 and 240.58±126.63 in Biosca with AT-MSCs, 377.97±131.00 and 413.21±177.84 in Biosca, and 40.22±54.06 and 33.69±2.03 in empty defects. No significant differences were observed among the treated defects related to the use of cells, cell source or OVX and Sham for all evaluated parameters. In conclusion, all treatments induced meaningful higher bone formation compared with empty defects, irrespective of OVX and Sham. We observed that Biosca is capable of increasing bone formation either in OVX or Sham rats, that is not enhanced by the association with MSCs.
14 May 2016 - 17 May 2016