Searchable abstracts of presentations at key conferences on calcified tissues
Bone Abstracts (2016) 5 P70 | DOI: 10.1530/boneabs.5.P70

ECTS2016 Poster Presentations Bone development/growth and fracture repair (35 abstracts)

Is heparin effective for the controlled delivery of high dose bone morphogeneic protein-2?

In Sook Kim 1 , Ri Youn Kim 2 & Soon Jung Hwang 2,

1Dental Research Institute, Seoul National University, Seoul, Republic of Korea; 2Department of Maxillofacial Cell and Developmental Biology, School of Dentistry, Seoul National University, BK21 2nd Program for Craniomaxillofacial Life Science, Seoul, Republic of Korea; 3Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Republic of Korea.

Sustained release of bone morphogeneic protein (BMP)-2 by heparin-contained biomaterials is advantageous for bone tissue regeneration using low dose BMP-2. However, its effect in high dose BMP-2 is still unclear and should be clarified considering the clinical use of high dose of BMP-2 in spine and oral surgery. This study aimed to evaluate the efficacy of heparin-conjugated collagen sponge (HCS) in high dose BMP-2 delivery by investigating in vivo initial osteogenic regulation and bone healing over time passage to 12 weeks in comparison with that of an absorbable collagen sponge (ACS). The in vitro BMP-2 release profile in the HCS exhibited a lower burst followed by a sustained release of BMP-2, while that of the ACS showed an initial burst phase only. As a result of lower burst, the HCS-BMP group showed more expression of bone-forming/resorbing markers and more activation of osteoclasts than the ACS-BMP group within the scaffold of defect at 7 day, which is presumed to a retention of relatively higher amount of BMP-2. However, the surrounding calvaria were less resorbed in HCS-BMP group, compared to the aggressive resorptive response in the ACS-BMP group. Micro-computed tomography and histology revealed that HCS-BMP guided more effective bone regeneration of central defect during time passage by inducing minor ossification at defect exterior while ACS-BMP exhibited excessive ossification at the defect exterior. These results showed that HCS-mediated BMP-2 delivery at high dose has advantages over ACS including less early resorption of surrounding bone tissue and higher efficacy in compact bone regeneration over a longer period, highlighting a clinical feasibility.

Volume 5

43rd Annual European Calcified Tissue Society Congress

Rome, Italy
14 May 2016 - 17 May 2016

European Calcified Tissue Society 

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