Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp41 | Bone development/growth and fracture repair | ECTS2014

Exosomes derived from human platelet lysate affect MSC functions in vitro

Torreggiani Elena , Perut Francesca , Roncuzzi Laura , Baldini Nicola

Introduction: Despite the popularity of platet lysate (PL) treatments in orthopaedics, the mechanism of action and the effectiveness of this therapeutic tool is still controversial. So far, the activity of PL has been associated with different growth factors (GFs) released upon platelet degranulation. However, PL activity might also be due to the efficient cell to cell transport system of GF and other bioactive molecules by their encapsulation into exosomes. In this study, we ...

ba0005cabs.op2.2 | Oral Poster Talks | ECTS2016

Mesenchymal stromal cells promote osteosarcoma stemness and migratory potential via IL-6 secretion

Cortini Margherita , Avnet Sofia , Bonuccelli Gloria , Baldini Nicola

Osteosarcoma (OS) is an aggressive malignancy characterized by a high relapse rate despite multiagent chemotherapy. As for other cancers, OS microenvironment contributes to tumor initiation, growth, and metastasis. We consider bone marrow mesenchymal stromal cells (MSC) as a major nontumor component of OS microenvironment, and have previously found that the interaction between MSC and tumor cells is bidirectional, i.e. tumor cells can modulate their peripheral environment that...

ba0005p120 | Cancer and bone: basic, translational and clinical | ECTS2016

The role of acidic microenvironment in the context of osteolytic carcinomas

Di Pompo Gemma , Lemma Silvia , Canti Lorenzo , Gillies Robert , Baldini Nicola , Avnet Sofia

The microenvironment of osteolytic metastases includes carcinoma cells derived from the primary lesion as well as bone-forming and bone-resorbing cells, namely osteoblasts (OB) and osteoclasts (OC). At this site, both the high glycolysis of cancer cells, and the bone resorption process result in a very acidic milieu. This, in turn, induces the surrounding stroma and OB to secrete pro-inflammatory cytokines and growth factors that promote tumorigenesis and cancer-associated ost...

ba0005p202 | Cell biology: osteoclasts and bone resorption | ECTS2016

The effect of potassium citrate on human primary osteoclasts in vitro

Torreggiani Elena , Massa Annamaria , Di Pompo Gemma , Granchi Donatella , Baldini Nicola

An increasing amount of scientific evidence suggests that western diet is a risk factor for osteopenia and osteoporosis. Indeed, metabolic acidosis, occurring after high levels of protein intake, may adversely impact on the skeleton by disrupting calcium metabolism, and leads to a decrease of bone mineral density (BMD). Currently, the prevention and treatment of osteopenia and osteoporosis are mainly based on lifestyle modifications (i.e. exercise, quitting smoking and ensurin...

ba0005p121 | Cancer and bone: basic, translational and clinical | ECTS2016

MCT1 as a novel target for the treatment of osteolytic bone metastases

Avnet Sofia , Lemma Silvia , Di Pompo Gemma , Sboarina Martina , Porporato Paolo , Perez-Escuredo Jhudit , Sonveaux Pierre , Baldini Nicola

Bone metastasis (BM) is a dismal complication of cancer, occurring frequently in patients with advanced breast carcinoma. During metastatic progression, carcinoma cells harness osteoclast (OC) activity, promoting osteolysis. To adapt to hypoxia and/or to support proliferation, carcinoma cells adopt primarily glycolysis for energy production, therefore releasing lactic acid in the microenvironment through monocarboxylate transporter 4 (MCT4). Stressed by tumor cells, osteoblast...