Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp180 | Cell biology: osteoblasts and bone formation | ECTS2013

N-cadherin governs age-related osteoprogenitor cell determination in mice through modulation of Wnt5a and Wnt10b

Hay Eric , Dieudonne Francois-Xavier , Marty Caroline , Marie Pierre J

Senile osteoporosis and age-related osteopenia are associated with decreased osteoblastogenesis and increased bone marrow adipogenesis. The mechanisms controlling the fate determination of osteoblast to adipocyte differentiation of bone marrow stromal cells (BMSC) during aging are not known. We and others previously showed that the cell-cell adhesion molecule N-cadherin (N-Cadh) expressed in osteoblasts controls bone formation, but little is known about its role in BMSC fate d...

ba0003pp179 | Chondrocytes and cartilage | ECTS2014

Osteoclasts inhibit the Wnt canonical pathway in chondrocytes

Cherifi Chahrazad , Bouaziz Wafa , Cohen-Solal Martine , Hay Eric

Purpose: Osteoclastogenesis is enhanced in osteoarthritis (OA). We have demonstrated that cartilage breakdown is reduced when osteoclastogenesis is inhibited in murine models. Wnt activity, known to regulate the bone and chondrocyte cell function, might contribute to the mechanisms that promote cartilage catabolism. Our purpose was to evaluate whether osteoclast-secreted molecules affect the chondrocyte metabolism and assess the contribution of Wnt pathway.<p class="abstex...

ba0001pp17 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Bone is the main target of activation of Canonical Wnt pathway in osteoarthritis

Funck-Brentano Thomas , Bouaziz Wafa , Geoffroy Valerie , Hannouche Didier , Marty Caroline , Hay Eric , Cohen-Solal Martine

Objective: Wnt/β-catenin pathway is a main regulator of bone remodeling, but might be inhibited in cartilage in osteoarthritis (OA). We here investigated the effect of mechanical loading in Wnt activation and the expression of Wnt antagonists in the joint tissues.Methods: Topgal mice were used. Mice underwent partial meniscectomy (Mnx) and sacrificed at 4, 6, and 9 weeks. Dissected knees were scanned by microCT and then prepared for cryosectioning t...

ba0001pp256 | Chondrocytes and cartilage | ECTS2013

Sclerostin preserves chondrocyte maintenance by modulating the crosstalk between canonical and non-canonical Wnt pathways

Bouaziz Wafa , Funck-Brentano Thomas , Lin Hilene , Ea Hang-Korng , Hay Eric , Cohen-Solal Martine

Aim: Wnt/β-catenin pathway promotes cartilage breakdown in osteoarthritis. We have previously shown that sclerostin preserves chondrocyte maintenance in vitro by reducing chondrocyte catabolism through the inhibition of Wnt/β-catenin pathway. However, sclerostin restores partially the expression of the anabolic genes. We therefore investigated the effect of sclerostin in the activation of Wnt non canonical pathways mediated by Ca2+/CaMKII, JNK, an...

ba0001pp468 | Other diseases of bone and mineral metabolism | ECTS2013

Miglustat therapy normalizes bone mass in a mouse model of cystic fibrosis

Henaff Carole Le , Hay Eric , Velard Frederic , Marty Caroline , Marie Pierre J , Jacquot Jacky P

Brittle bones have been reported in children, adolescents and adults with cystic fibrosis (CF), independently of sex; this has been termed CF-related bone disease. In CF patients with the F508del mutation in the (Cftr) gene, vertebral fractures and the subsequent dorsal kyphosis decrease pulmonary function, thus accelerating the course of the disease. Mice with the homozygous F508del mutation in CFTR develop a severe osteopenic phenotype early on, in both sexes (Le He...

ba0003ht4 | (1) | ECTS2014

Sclerostin prevents mice from osteoarthritis despite high subchondral bone accretion

Bouaziz Wafa , Funck-Brentano Thomas , Lin Hilene , Marty Caroline , Come Dominique , Hay Eric , Cohen-Solal Martine

Purpose: Sclerostin, a Wnt inhibitor produced by osteocytes that regulate bone remodelling, might be involved in cartilage metabolism. Therefore, we assessed the effect of sclerostin in osteoarthritis using SOST-deficient mice.Methods: SOST-KO and wild type (WT) mice underwent partial meniscectomie (Mnx). Mice were sacrificed at 4, 6 or 9 weeks after Mnx to analyze i) bone volume (BV/TV) at the femoral condyle, ii) osteophyte volume (microCT), iii) carti...

ba0003pp15 | Arthritis and other joint diseases: translational and clinical | ECTS2014

Bone secreted dickkopf-related protein 1 ameliorates osteoarthritis in mice

Funck-Brentano Thomas , Bouaziz Wafa , Marty Caroline , Geoffroy Valerie , Hay Eric , Cohen-Solal Martine

Objective: Cartilage loss and subchondral bone changes are hallmarks of osteoarthritis (OA). The Wnt family is involved in the regulation of bone and cartilage. We have shown that the Wnt/β-catenin pathway is activated in bone during OA, but the effect of its inhibition in bone in cartilage remodeling is unknown. We here investigated the impact of the bone-specific inhibition of Wnt during the development of OA.Methods: Partial meniscectomy (Mnx) wa...