Searchable abstracts of presentations at key conferences on calcified tissues
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European Calcified Tissue Society Congress 2014

Prague, Czech Republic
17 May 2014 - 20 May 2014

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European Calcified Tissue Society Annual Congress, 17 - 20 May 2014; Prague, Czech Republic

Poster Presentations

Chondrocytes and cartilage

ba0003pp177 | Chondrocytes and cartilage | ECTS2014

The transcription factor Foxc1 controls endochondral ossification through the direct regulation of PTHrP and Cola10a1 expression under the partnership with Gli2 and Runx2

Yoshida Michiko , Hata Kenji , Takashima Rikako , Iseki Sachiko , Yamamoto-Takano Teruko , Nishimura Riko , Yoneda Toshiyuki

Endochondral ossification is regulated by various transcription factors in a temporo-spatial manner. Identification of novel transcription factors involved in chondrogenesis would facilitate to uncover the molecular basis of endochondral ossification. To approach this, we generated transgenic mice in which chondrocytes were specifically fluorescence-labeled with Venus gene using Col2a1 gene promoter, thereby allowing us to isolate Venus-positive chondrogenic cells using FACS A...

ba0003pp178 | Chondrocytes and cartilage | ECTS2014

LDL receptor-related protein 5 governs Wnt-mediated osteoarthritic cartilage destruction

Huh Yunhyun , Koh Jeong-Tae , Kook Min-Suk , Ryu Je-Hwang

Introduction: Wnt ligands bind to LDL receptor-related protein (LRP) 5 or −6, triggering a cascade of downstream events that include β-catenin signaling. Here, we explored the roles of LRP5 in interleukin (IL)-1β- or Wnt-mediated osteoarthritic (OA) cartilage destruction in mice.Methods: The expression levels of LRP5, Type II collagen, and catabolic factors were determined in mouse articular chondrocytes, human OA cartilage, and mouse exp...

ba0003pp179 | Chondrocytes and cartilage | ECTS2014

Osteoclasts inhibit the Wnt canonical pathway in chondrocytes

Cherifi Chahrazad , Bouaziz Wafa , Cohen-Solal Martine , Hay Eric

Purpose: Osteoclastogenesis is enhanced in osteoarthritis (OA). We have demonstrated that cartilage breakdown is reduced when osteoclastogenesis is inhibited in murine models. Wnt activity, known to regulate the bone and chondrocyte cell function, might contribute to the mechanisms that promote cartilage catabolism. Our purpose was to evaluate whether osteoclast-secreted molecules affect the chondrocyte metabolism and assess the contribution of Wnt pathway.<p class="abstex...

ba0003pp180 | Chondrocytes and cartilage | ECTS2014

Selective targeting of bone and cartilage with multivalent dendritic polyanions

Reimann Sabine , Groger Dominic , Lim Ngee Han , Licha Kai , Welker Pia , Schneider Tobias , Nagase Hideaki , Fratzl Peter , Haag Rainer

Background and objective: Targeting bone and cartilage includes approaches using tetracyclines, peptide conjugates, as well as anionic moieties like bisphosphonates. However, the selective targeting of the different compartments still remains a challenge. In previous studies native and demineralized ovine bone was used to analyze the affinity of polyanions derived from dendritic polyglycerol (dPG) toward hydroxyapatite and collagen. Whereas the neutral polymer did not show any...

ba0003pp181 | Chondrocytes and cartilage | ECTS2014

Hyperbaric oxygen treatment suppresses Wnt/β-catenin signaling and matrix metalloproteinases in degenerated human intervertebral disc cells

Niu Chi-Chien , Lin Song-Shu , Yuan Li-Jen , Yang Chuen-Yung , Chen Wen-Jer , Ueng Steve WN

Background: The activation of Wnt/β-catenin signaling promotes cellular senescence and induces matrix metalloproteinases (MMPs) expression in intervertebral disc (IVD). However, little is known about the effects of hyperbaric oxygen (HBO) on the Wnt/β-catenin signaling and MMPs expression in degenerated human IVDs.Materials and methods: Nucleus pulposus cells (NPCs) were separated from the degenerated disc nucleus tissue by performing sequentia...

ba0003pp182 | Chondrocytes and cartilage | ECTS2014

Hyperbaric oxygen therapy reduces the catabolic pathway mediated by toll-like receptors in human osteoarthritic chondrocytes

Yuan Li-Jen , Lin Song-Shu , Niu Chi-Chien , Yang Chuen-Yung , Ueng Steve WN , Chen Wen-Jer

Background: Expression of toll-like receptor (TLR) 2 and 4 was up-regulated in lesional areas of OA cartilage. Hyperbaric oxygen (HBO) reduces the TLR signalling pathway in multiple organ failures. However, little is known about the effects of HBO on the catabolic pathway mediated by TLRs in human osteoarthritic (OA) chondrocytes.Materials and methods: OA chondrocytes were separated from the knees of OA patients by performing sequential enzymatic digesti...

ba0003pp183 | Chondrocytes and cartilage | ECTS2014

Meniscus – Cartilage paracrine crosstalk in osteoarthritis

Samara Stavroula , Chatzopoulou Elisavet , Melas Ioannis , Messinis Dimitris , Dailiana Zoe , Kollia Panagoula , Alexopoulos Leonidas

Introduction: Meniscus plays an essential role in knee joint function providing stability and load transmission. In osteoarthritis (OA), a joint disease characterized by chronic synovitis and cartilage degeneration, pathological changes in the menisci are observed. However, whether menisci contribute to the progression of OA, the underlying mechanism for meniscus-cartilage communication is still unclear. In this study we analyzed systematically the response of meniscus and car...

ba0003pp184 | Chondrocytes and cartilage | ECTS2014

Therapeutic education of patients treated for osteoarthritis: medical advice and assessment of patients knowledge level

Nassar Kawtar , Janani Saadia , Rachidi Wafae , Mkinsi Ouafa

Introduction: Osteoarthritis is the most common and debilitating degenerative arthropathy. Prevention and patient education are part of the international recommendations. A survey was realized with 43 medical specialists at the Hospital University of Casablanca, about their point of views on the role of therapeutic education for patients with osteoarthritis of the lower limb. Doctors justified the importance of patient education.Study purpose: Evaluate o...

ba0003pp185 | Chondrocytes and cartilage | ECTS2014

Disturbed cartilages of the mandible in achondroplasia are associated with defective mandible shape and position

Duplan Martin Biosse , Di Rocco Federico , Heuze Yann , Gaudas Emilie , Komla-Ebri Davide , Kaci Nabil , Benoist-Lasselin Catherine , Legeai-Mallet Laurence

FGFR3 activating mutations are responsible for achondroplasia (ACH), the most common form of dwarfism. ACH clinical features include short stature, midface hypoplasia, frontal bossing and prognathism and both endochondral and membranous ossifications are disturbed. It is unknown if abnormal mandibles are present in ACH. To date, it is believed that primary (Meckel’s) and secondary (angular and condylar) cartilages play important roles in determining the final shape and po...