Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp212 | Cell biology: osteoblasts and bone formation | ECTS2013

Bone-forming cultures of rat and mouse calvarial osteoblasts: key differences in protocols

Orriss Isabel , Hajjawi Mark , Huesa Carmen , MacRae Vicky , Arnett Timothy

The in vitro culture of calvarial osteoblasts from neonatal rodents remains an important method for studying the regulation of osteoblast function. Widespread use of transgenics has created a particular need for a reliable, simple method that allows the differentiation and bone-forming activity of mouse osteoblasts to be studied directly. We have established such a method and have identified key differences in optimal culture conditions between mouse and rat osteoblas...

ba0001oc6.1 | Mineralisation and energy metabolism | ECTS2013

Npp1 is a key regulator of skeletal and soft tissue mineralisation

Hajjawi Mark , MacRae Vicky , Huesa Carmen , Millan Jose Luis , Poulet Blandine , Arnett Timothy , Orriss Isabel

Ecto-nucleotide pyrophosphatase/phosphodiesterases (NPPs) hydrolyse nucleotide triphosphates to the corresponding nucleotide monophosphate and the mineralisation inhibitor, pyrophosphate (PPi). This investigation examined the role of NPP1 in bone and soft tissue mineralisation using a mouse model lacking NPP1(Enpp1−/−). At physiological pH 7.35, cultured Enpp1−/...

ba0001pp234 | Cell biology: osteoclasts and bone resorption | ECTS2013

Do ecto-nucleotidases play a role in the regulation of osteoclast function?

Hajjawi Mark , MacRae Vicky , Huesa Carmen , Millan Jose Luis , Arnett Timothy , Orriss Isabel

Extracellular nucleotides stimulate both the formation and resorptive activity of osteoclasts. Ecto-nucleotide pyrophosphatase/phosphodiesterases (NPPs) hydrolyse extracellular nucleotide triphosphates to their corresponding monophosphate and pyrophosphate (PPi). We investigated if osteoclasts express functional NPPs and whether Enpp1 gene deletion influenced osteoclast formation and activity. Osteoclasts were formed from the bone marrow of 8 and 15 week old knockou...

ba0001oc6.2 | Mineralisation and energy metabolism | ECTS2013

Deficiency of the bone mineralisation inhibitor NPP1 protects against obesity and diabetes

Huesa Carmen , Morton Nicholas M , Ferron Mathieu , Karsenty Gerard , Millan Jose Luis , Ahmed Faisal , Farquharson Colin , MacRae Vicky E

Bone has recently emerged as a novel endocrine organ regulating glucose metabolism. Ectonucleotide pyrophosphatase/phosphodiesterase-1 (NPP1) controls bone mineralisation by generating the mineralisation inhibitor pyrophosphate. In clinical studies increased activity of NPP1 has been found in patients with insulin resistance, and it has been shown to directly inhibit the insulin receptor. We hypothesised that mice lacking NPP1 (Enpp1−/−) would exhibit im...

ba0001oc6.6 | Mineralisation and energy metabolism | ECTS2013

An emerging role of phospho1 in the regulation of energy metabolism

Oldknow Karla , Morton Nik Morton's , Yadav Manisha , Rajoanah Sophie , Huesa Carmen , Bunger Lutz , Ferron Mathieu , Karsenty Gerard , MacRae Vicky , Milan Jose Luis , Farquharson Colin

Genetic approaches to bone physiology utilising judicious gain and loss of function models have identified bone as an endocrine organ, being involved in the regulation of energy metabolism and reproduction. Recent advances expand our understanding and identify a new and unconventional role of bone beyond its classical functions. PHOSPHO1 is a bone specific phosphatase with a recognised role in bone mineralisation, but our present studies have now identified a novel role for PH...