Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp180 | Cell biology: osteoblasts and bone formation | ECTS2013

N-cadherin governs age-related osteoprogenitor cell determination in mice through modulation of Wnt5a and Wnt10b

Hay Eric , Dieudonne Francois-Xavier , Marty Caroline , Marie Pierre J

Senile osteoporosis and age-related osteopenia are associated with decreased osteoblastogenesis and increased bone marrow adipogenesis. The mechanisms controlling the fate determination of osteoblast to adipocyte differentiation of bone marrow stromal cells (BMSC) during aging are not known. We and others previously showed that the cell-cell adhesion molecule N-cadherin (N-Cadh) expressed in osteoblasts controls bone formation, but little is known about its role in BMSC fate d...

ba0001pp17 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Bone is the main target of activation of Canonical Wnt pathway in osteoarthritis

Funck-Brentano Thomas , Bouaziz Wafa , Geoffroy Valerie , Hannouche Didier , Marty Caroline , Hay Eric , Cohen-Solal Martine

Objective: Wnt/β-catenin pathway is a main regulator of bone remodeling, but might be inhibited in cartilage in osteoarthritis (OA). We here investigated the effect of mechanical loading in Wnt activation and the expression of Wnt antagonists in the joint tissues.Methods: Topgal mice were used. Mice underwent partial meniscectomy (Mnx) and sacrificed at 4, 6, and 9 weeks. Dissected knees were scanned by microCT and then prepared for cryosectioning t...

ba0001pp68 | Bone development/growth and fracture repair | ECTS2013

Long bone phenotypic analyzes of a RANK transgenic mouse line

Baaroun Vanessa , Coudert Amelie , Marty Caroline , Castaneda Beatriz , Berdal Ariane , Descroix Vianney

Introduction: Bone metastasis pathophysiology is currently defined as a vicious circle. Indeed, tumor cells co-express RANK-L and its receptor, RANK, allowing their own proliferation and peritumoral bone resorption necessary to the lesion expansion. Odontogenic tumors, which occur within the jaw, seem to present the same pathophysiological characteristics. Thus, studying the impact on bone of RANK over-expression by the osteoclasts would permit a better understanding of those ...

ba0001pp468 | Other diseases of bone and mineral metabolism | ECTS2013

Miglustat therapy normalizes bone mass in a mouse model of cystic fibrosis

Henaff Carole Le , Hay Eric , Velard Frederic , Marty Caroline , Marie Pierre J , Jacquot Jacky P

Brittle bones have been reported in children, adolescents and adults with cystic fibrosis (CF), independently of sex; this has been termed CF-related bone disease. In CF patients with the F508del mutation in the (Cftr) gene, vertebral fractures and the subsequent dorsal kyphosis decrease pulmonary function, thus accelerating the course of the disease. Mice with the homozygous F508del mutation in CFTR develop a severe osteopenic phenotype early on, in both sexes (Le He...

ba0003ht4 | (1) | ECTS2014

Sclerostin prevents mice from osteoarthritis despite high subchondral bone accretion

Bouaziz Wafa , Funck-Brentano Thomas , Lin Hilene , Marty Caroline , Come Dominique , Hay Eric , Cohen-Solal Martine

Purpose: Sclerostin, a Wnt inhibitor produced by osteocytes that regulate bone remodelling, might be involved in cartilage metabolism. Therefore, we assessed the effect of sclerostin in osteoarthritis using SOST-deficient mice.Methods: SOST-KO and wild type (WT) mice underwent partial meniscectomie (Mnx). Mice were sacrificed at 4, 6 or 9 weeks after Mnx to analyze i) bone volume (BV/TV) at the femoral condyle, ii) osteophyte volume (microCT), iii) carti...

ba0003pp15 | Arthritis and other joint diseases: translational and clinical | ECTS2014

Bone secreted dickkopf-related protein 1 ameliorates osteoarthritis in mice

Funck-Brentano Thomas , Bouaziz Wafa , Marty Caroline , Geoffroy Valerie , Hay Eric , Cohen-Solal Martine

Objective: Cartilage loss and subchondral bone changes are hallmarks of osteoarthritis (OA). The Wnt family is involved in the regulation of bone and cartilage. We have shown that the Wnt/β-catenin pathway is activated in bone during OA, but the effect of its inhibition in bone in cartilage remodeling is unknown. We here investigated the impact of the bone-specific inhibition of Wnt during the development of OA.Methods: Partial meniscectomy (Mnx) wa...