Searchable abstracts of presentations at key conferences on calcified tissues
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European Calcified Tissue Society Congress 2013

Lisbon, Portugal
18 May 2013 - 22 May 2013

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European Calcified Tissue Society Congress 2013, 18 - 22 May 2013; Lisbon, Portugal

Poster Presentations

Bone development/growth and fracture repair

ba0001pp59 | Bone development/growth and fracture repair | ECTS2013

The effect of mTORC1 on postnatal skeletal development

Matthews Mary , Zannettino Andrew , Fitter Stephen , Martin Sally

Mammalian target of rapamycin (mTOR) is a serine–threonine kinase that plays a central role in a number of key cellular pathways that have been previously implicated in bone formation. mTOR mediates these diverse roles by forming two multi-protein complexes, mTORC1 and mTORC2, each of which is defined by unique proteins raptor and rictor respectively.Studies from our laboratory have previously demonstrated that inhibition of mTORC1 increases the ost...

ba0001pp60 | Bone development/growth and fracture repair | ECTS2013

MEK inhibitors in fracture healing and NF1 pseudarthrosis

Little David , El-Hoss Jad , Kollind Mille , Deo Nikita , McDonald Michelle , Sullivan Kate , Little Chris , Schindeler Aaron

Neurofibromatosis type 1 (NF1) is a genetic disorder with an incidence of 1/3000. Inactivating mutations in the NF1 gene cause Ras-MEK overstimulation, and predisposes NF1 patients to cancer. A new generation of MEK inhibitors (PD0325901 and AZD6244) are under clinical trials in cancer patients, including NF1 patients. Congenital pseudarthrosis of the tibia is a major complication for NF1 patients, and associates with loss-of-heterozygosity of the NF1 gene. The primary aim of ...

ba0001pp61 | Bone development/growth and fracture repair | ECTS2013

Vascularisation and progenitor cells of primary and secondary ossification centres in the human growth plate

Walzer Sonja M , Cetin Erdal , Grubl-Barabas Ruth , Sulzbacher Irene , Ruger Beate , Girsch Werner , Windhager Reinhard , Fischer Michael B

The switch from a cartilage template to bone during endochondralossification of the growth plate requires dynamic and close interaction between the cartilage and the developing vascular structures. Vascular invasion of hypertrophic cartilage, with blood vessels coming from the bone collar, serves to bring in osteoblast- andendothelial precursor cells along with chondroclasts and their precursors into future ossification centres of the growth plate.Potent...

ba0001pp62 | Bone development/growth and fracture repair | ECTS2013

Intermittent administration of parathyroid hormone (1–34) may induce the formation of cementum and bone: a histological study in rats

Vasconcelos Daniel , Marques Marcelo , Barros Silvana , Vasconcelos Any Carolina , Benatti Bruno , Novaes Pedro

The aim of this study was to evaluate the effect of anabolic PTH on periodontal repair and mandibular bone defect in rats. Fenestration defects were created unilaterally of lower first molars in Wistar rats (n=32), and both periodontal ligament and cementum were removed. Animals were treated 3 times a week and then assigned to four groups (n=8): i) C14 – placebo administration for 14 days; ii) P14 – PTH administration for 14 days; iii) C21 – pl...

ba0001pp63 | Bone development/growth and fracture repair | ECTS2013

Microtomography analysis of bone formation in calvarial defects filled with gelatin sponge or fibrin glue

de Santana Santos Thiago , Lopes Helena Bacha , Almeida Adriana Luiza , Beloti Marcio Mateus , Rosa Adalberto Luiz

Bone tissue engineering relies on the combination of scaffolds and cells aiming to heal bone defects. Among the many candidates for scaffolds, gelatin sponge (Gelfoam®) and fibrin glue (Tissucol®) are of interest due to the well-established biocompatibility and the ability to be loaded with cells. Here, we evaluated their potential to stimulate bone formation by microtomography analysis. For this, 5-mm unilateral calvarial defect was created in rats deeply anesthetiz...

ba0001pp65 | Bone development/growth and fracture repair | ECTS2013

A novel biocompatible BMP6 carrier device for bone regeneration

Grgurevic Lovorka , Erjavec Igor , Jankolija Morana , Popek Irena , Olic Anamarija , Topic Smiljka Vikic , Verbanac Donatella , Peric Mihaela , Oppermann Hermann , Vukicevic Slobodan

Bone morphogenetic protein 6 (BMP6) is a member of TGF-β superfamily with a high potential to induce new bone and cartilage. Here we demonstrate that BMP6 compared to the BMP7 paralog has unique biological properties. Previously, we showed that BMP6 is more active at lower amounts then BMP7 because of increased resistance to noggin due to lysine in position 60. Next, we discovered that BMP6 binds to blood coagulum components, which when modified with calcium salt and a fi...

ba0001pp66 | Bone development/growth and fracture repair | ECTS2013

The P2Y2 receptor restrains BMD during development

Syberg Susanne , Ellegaard Maria , Schwarz Peter , Jorgensen Niklas Rye

The P2Y2 receptor is a G-protein-coupled receptor and the natural ligands (ATP/UTP) strongly inhibit mineralized bone nodule formation by osteoblasts in vitro. We have earlier shown that overexpression of the P2Y2 receptor in vivo resulted in decreased bone mineral density (BMD), partially due to increased bone resorption, but also decreased formation (detected by serum bone markers and bone histomorphometry).This ...

ba0001pp67 | Bone development/growth and fracture repair | ECTS2013

Hip fracture induces a transient appearance of circulating mesenchymal stem cells

Heino Terhi J , Sederquist Bettina , Aro Hannu T

We have previously demonstrated the presence of circulating mesenchymal stem cells (MSCs) in fracture patients. However, the exact time for their appearance in circulation remains unknown. Nine patients (five females, four males, age 70±12 years, range 55–89 years) with a fresh femoral neck fracture were recruited in the study, which was approved by the local ethical committee. Bone marrow (BM) sample drawn from the iliac crest of all patients served as the individua...

ba0001pp68 | Bone development/growth and fracture repair | ECTS2013

Long bone phenotypic analyzes of a RANK transgenic mouse line

Baaroun Vanessa , Coudert Amelie , Marty Caroline , Castaneda Beatriz , Berdal Ariane , Descroix Vianney

Introduction: Bone metastasis pathophysiology is currently defined as a vicious circle. Indeed, tumor cells co-express RANK-L and its receptor, RANK, allowing their own proliferation and peritumoral bone resorption necessary to the lesion expansion. Odontogenic tumors, which occur within the jaw, seem to present the same pathophysiological characteristics. Thus, studying the impact on bone of RANK over-expression by the osteoclasts would permit a better understanding of those ...

ba0001pp69 | Bone development/growth and fracture repair | ECTS2013

The sealing zone is not required for mineralized cartilage resorption during endochondral ossification and growth of long bone

Touaitahuata Heiani , Cres Gaelle , Blangy Anne

Introduction: Osteoclasts are the only cells with the capacity to degrade mineralized matrices, such as bone and calcified cartilage. During bone remodeling, osteoclasts secrete protons to achieve the acidic dissolution of hydroxyapatite to make bone collagen amenable to digestion by the proteases they produce. This requires the sealing zone, a ring of densely packed podosomes that surrounds the ruffled border, which is the secretion apparatus of the bone resorbing osteoclasts...

ba0001pp70 | Bone development/growth and fracture repair | ECTS2013

A casein-based diet leads to a better bone status than a soy protein-based diet during moderate protein restriction in growing mice

Rouy Emilien , Laroche Norbert , Blachier Francois , Tome Daniel , Vico Laurence , Blais Anne

This study aims at determining if casein would lead to a better bone status than soy in the context of a moderate protein restriction (6% of total energy intake) in growing mice.Ten-week-old female Balb/C mice were divided in four groups of 15 animals. Two groups received 6% of their energy intake as protein, one as casein and the other as soy protein. The third group was a normal-protein control receiving 20% soy protein. The last group (positive contro...

ba0001pp71 | Bone development/growth and fracture repair | ECTS2013

Accuracy errors in longitudinal QCT measurements of cortical thickness bone mineral density and bone mineral content using different segmentation techniques: a simulation study

Gerner Bastian , Museyko Oleg , Topfer Dominique , Engelke Klaus

Introduction: The quantification of cortical BMD and thickness in QCT images remains challenging due to the limited spatial resolution of CT scanners. We simulated the impact of longitudinal cortical BMD and thickness changes on accuracy of cortical measurements using three different segmentation algorithms.Methods: A step function of varying width d (cortical thickness) and height (cortical BMD) and an additional step representing trabecular BMD was con...

ba0001pp72 | Bone development/growth and fracture repair | ECTS2013

Calcium phosphate cement/strontium enhances bone formation in the metaphyseal osteoporotic fracture

Khassawna Thaqif El , Ray Seemun , Thormann Ulrich , Lips Katrin , Gelinsky Michael , Schumacher Matthias , Langheinrich Alexander Claus , Schnettler Reinhard , Alt Volker

Objective: Oosteoporoti fractures are a growing problem especially in aged societies of industrialized countries. Therefore, a clinical demand for synthetic bone graft substitutes is increasing. Despite the general success CPC showed clinically, development of CPC-based material by adding stronsium could improve its suitability to treat osteoporotic fractures.Methods: Sprague–Dawley rates model of induced osteoporosis via multi-deficiencies diet com...

ba0001pp73 | Bone development/growth and fracture repair | ECTS2013

Feasibility of local CD133+ cell transplantation to avoid non-unions in biological impaired bone healing

Dienelt Anke , Sass Andrea F , Preininger Bernd , Schmidt-Bleek Katharina , Duda Georg N

The clinical orthopaedic problem of delayed healing or non-union after complex fractures affects 5–10% of all patients, especially within the elderly population. Recently several in vitro studies showed that CD133+ cells bare angiogenic capacities and contribute to a better outcome concerning ischemia induced angiogenesis in vivo. A local administration of these specific cells to the fracture gap appears feasible as a new treatment option for biological ...

ba0001pp74 | Bone development/growth and fracture repair | ECTS2013

Metaphyseal fracture healing in a sheep model of low turnover osteoporosis induced by hypothalamic–pituitary disconnection

Bindl Ronny , Oheim Ralf , Pogoda Pia , Beil Frank Timo , Gruchenberg Katharina , Reitmaier Sandra , Wehner Tim , Calcia Enrico , Radermacher Peter , Claes Lutz , Amling Michael , Ignatius Anita

We recently established a large animal model of osteoporosis in sheep using hypothalamic–pituitary disconnection (HPD). As central regulation is important for bone metabolism, HPD-sheep developed severe osteoporosis because of low bone turnover. In this study we investigated metaphyseal fracture healing in HPD-sheep. To elucidate potential pathomechanisms, we included a treatment group receiving thyroxine (T4) and 17β-estradiol. Because clinically osteopor...

ba0001pp75 | Bone development/growth and fracture repair | ECTS2013

Interplay of physical and biological cues in the regeneration of critical-sized bone defects

Cipitria Amaia , Reichert Johannes C , Lange Claudia , Schell Hanna , Mehta Manav , Wagermaier Wolfgang , Zaslansky Paul , Fratzl Peter , Hutmacher Dietmar W , Duda Georg N

The transplantation of autologous bone graft represents the ‘gold standard’ treatment for large bone defects, despite the harvesting co-morbidity and limited availability. An alternative scaffold-based approach is presented. Our aim was to investigate to what degree structured scaffolds alone, or in combination with biological stimuli, allow guiding tissue regeneration. Scaffolds consisting of medical-grade polycaprolactone and tricalcium phosphate microparticles, co...

ba0001pp76 | Bone development/growth and fracture repair | ECTS2013

The effect of post-natal (childhood) obesity on skeletal development

Monsonego-Ornan Efrat , Simsa-Maziel Stav , Zareski Janna , Anpilov Sergey , Solomon Gili

Childhood obesity is a serious global public health problem, reaching 40% of children in developed countries. While the connection between under-nutrition and growth retardation is well documented, the opposite connection between over-nutrition and bone development was barely studied. Obese children grow faster in height than normal-weighed children, and prospective studies demonstrated an over-presentation of obese children amongst fracture cases. Yet, the cellular and molecu...

ba0001pp77 | Bone development/growth and fracture repair | ECTS2013

Histologic diagnosis of aluminum osteomalacia in renal failure rats

Teraki Yoshimi

Aim of the study: Animal studies have also been reported demonstrating enhanced development of osteomalacia and increased osteoids following long-term high-dose administration of Al compound in rats, especially in those with renal failure rats (Robertson et al.).For histologic detection of Al. aluminon and other staining reagents have usually been used but are not adequately sensitive and lack in specificity. We have recently developed a method ...

ba0001pp78 | Bone development/growth and fracture repair | ECTS2013

Sex-related differences of femur properties in silver foxes (Vulpes vulpes)

Tatara Marcin , Krupski Witold , Jakubczak Andrzej , Bienko Marek , Kostro Krzysztof

Considering limited information available on skeletal system properties in foxes, the aim of this study was to determine morphological, geometrical and densitometric parameters of femur obtained from males and females. The study was performed on 1-year old male (n=5) and female (n=6) silver foxes. Right femur was isolated and its weight and length were measured. Using quantitative computed tomography (QCT) technique and Somatom Emotion Siemens apparatus, bone...

ba0001pp79 | Bone development/growth and fracture repair | ECTS2013

Interrelationships between morphometric, densitometric and mechanical properties of mandible in 5-month old Polish Merino sheep

Krupski Witold , Tatara Marcin , Szabelska Anna , Luszczewska-Sierakowska Iwona , Tymczyna Barbara

The aim of the study was to evaluate interrelationships between morphometric, densitometric and mechanical properties of mandible in Polish Merino sheep. Males (n=7) were kept to slaughter age of 5 months of life to obtain mandible. After the slaughter, morphological properties of the mandible such as bone weight and length were determined. Using quantitative computed tomography (QCT) technique and Somatom Emotion, Siemens apparatus, volumetric bone mineral density (v...

ba0001pp80 | Bone development/growth and fracture repair | ECTS2013

Bone metabolism is influenced by serum 25-hydroxyvitamin D in healthy children

Czekuc-Kryskiewicz Edyta , Karczmarewicz Elzbieta , Jaworski Maciej , Czech-Kowalska Justyna , Gorska Anna , Konstantynowicz Jerzy , Pludowski Pawel , Piskorski Jaroslaw , Lorenc Roman

Introduction: Serum 25(OH)D concentrations for optimal bone metabolism in children is unknown. Only few data exist describing the effects of increasing serum 25(OH)D on bone metabolism markers.Aim: The aim of the study was to explore the association between serum 25(OH)D and bone metabolism markers in children.Patients and methods: Serum levels of bone formation (OC, P1NP) and bone resorption (CTx) markers (Cobas e411, Roche Diagno...

ba0001pp81 | Bone development/growth and fracture repair | ECTS2013

Characterization of an Y1R antagonist as a drug for bone regeneration

Alencastre Ines , Almeida Catarina , Leite Diana , Alves Cecilia , Sousa Daniela , Neto Estrela , Lamghari Meriem

Recently, Y1 receptor (Y1R) has arisen as a potential regulator in the local control of bone turnover. BIBP3226 is a potent Y1R selective antagonist that was successfully used in in vitro studies showing a positive impact in the benefit of bone turnover, thus providing good perspectives for its use as a pharmacological tool for bone regeneration.However, BIBP3226 behaviour in a complex milieu such as the bone compartment is unknown. As drugs can...

ba0001pp82 | Bone development/growth and fracture repair | ECTS2013

Measurement properties of radial and tibial speed of sound for screening bone health and fragility in 10–12 years old boys and girls

Rebocho Lurdes , Cardadeiro Graca , Zymbal Vera , Goncalves Ezequiel M , Sardinha Luis B , Baptista Fatima

The objective of this study was to analyze measurement properties of BeamMed Omnisense quantitative ultrasound (QUS) of the radial and tibial speed of sound (SoS) for assessing bone health and screening bone fragility in youth. Bone fragility was defined as low whole body less head bone mineral density (WBLH BMD) measured by DXA (first tertile, 95% CI: −1.1– (−0.9)) and as past history of fractures evaluated by questionnaire. The study was conducted with 319 n...

ba0001pp83 | Bone development/growth and fracture repair | ECTS2013

Trabecular micro-architecture of the proximal femur during post-natal growth

Djuric Marija , Milovanovic Petar , Djonic Danijela , Hahn Michael , Busse Bjoern , Amling Michael

In sharp contrast to the great interest in morphology of the proximal femur in aged individuals, there is a paucity of studies in children. To date, a comprehensive quantitative analysis of trabecular micro-architecture in various biomechanically relevant subregions of the immature proximal femur has been lacking. The aim of this study was to characterize postnatal development of trabecular bone in various regions of the human proximal femur. The study sample was comprised of ...

ba0001pp84 | Bone development/growth and fracture repair | ECTS2013

Variations in osteotoxic effects of cadmium on femoral bone structure after different routes of exposure

Martiniakova Monika , Chovancova Hana , Omelka Radoslav , Bobonova Ivana , Toman Robert

Cadmium (Cd) is regarded as a risk factor for various bone diseases in humans and experimental animals. To compare effects of different routes of Cd administration on femoral bone structure, ten 4-month-old male Wistar rats (group A) were injected intraperitoneally with a single dose of 2 mg CdCl2/kg body weight and killed 36 h after Cd had been injected. Ten 1-month-old male rats (group B) were dosed with a daily Cd intake of 30 mg CdCl2/l in drinking wa...

ba0001pp85 | Bone development/growth and fracture repair | ECTS2013

The retention and bioactivity of rhBMP-2 released from a bisphosphonate-linked hyaluronan-based hydrogel

Hulsart-Billstrom Gry , Yuen Pik-Kwan , Marsell Richard , Hilborn Jons , Larsson Sune , Ossipov Dmitri

Introduction: There are several disadvantages with the present carriers used in rhBMP-2 products including risk for immunological response, inefficient release and poor handling properties. Our aim was to examine the release of rhBMP-2 from a bisphosphonate-linked hyaluronan hydrogel with the hypothesis that it would cause a slower release.Methods: Triplicates of hydrogels with rhBMP-2 and with (HA-BP) or without (HA) bisphosphonate were prepared, after ...

ba0001pp86 | Bone development/growth and fracture repair | ECTS2013

The effect of incubation time of preformed injectable hydrogels on bone formation when used as carrier of rhBMP-2

Piskounova Sonya , Hulsart-Billstrom Gry , Gedda Lars , Bergman Kristoffer , Hilborn Jons , Larsson Sune , Bowden Tim

Introduction: Hydrogels has demonstrated efficacy as carriers for growth factors. Our aim was to investigate the effect of curing-time of modified hyaluronan on bone formation.Methods: Hydrogels with rhBMP-2 were cross-linked for 14 and 3 days, 5 h or 1 min before injection. Preformed gels were injected s.c. in 5 rats, the rats were killed after 5 weeks. Explanted samples were radiographed and scanned by pQCT.Results: Bone formatio...

ba0001pp87 | Bone development/growth and fracture repair | ECTS2013

Premixed calcium phosphate cement as a carrier for bone morphogenetic protein 2

Walters Nick , Hulsart-Billstrom Gry , Engqvist Hakan , Larsson Sune

Introduction: A BMP-2-containing premixed calcium phosphate cement (PCPC) could potentially provide the surgeon with an easy-to-handle alloplastic material with osteoinductive properties, accelerating bone ingrowth by stimulating osteogenic differentiation.Methods: The release profile of BMP-2 at 0.05 and 0.50 mg/ml from the PCPC was characterized over a period of 1 week using ELISA.Results: The PCPC released BMP-2 in a biphasic ma...

ba0001pp88 | Bone development/growth and fracture repair | ECTS2013

Low calcium intake aggravates the deleterious effects of an isocaloric low protein diet on bone material level properties during growth

Fournier Carole , Rizzoli Rene , Ammann Patrick

Low protein or low calcium intake are known to impair bone growth, but their combined effects on determinants of bone strength are not well understood. We investigated the influence of various protein and calcium containing diets on determinants of bone strength in growing rats.One-month-old female rats were fed isocaloric diets containing 10, 7.5 or 5% casein, with 1.1% (normal; NCa) or 0.2% calcium (low; LCa) during 8 weeks. Tibia midshaft geometry (ou...

ba0001pp89 | Bone development/growth and fracture repair | ECTS2013

Spirulina alga prevents impairment of peak bone mass acquisition induced by an isocaloric low protein diet

Fournier Carole , Rizzoli Rene , Ammann Patrick

New food strategies should be developed to fight against child malnutrition and growth retardation in developing countries. Spirulina alga, one of the richest sources of vegetable protein, contains all essential amino acids. It easily grows in tropical regions. We hypothesized that impaired peak bone mass acquisition (PBMA) caused by dietary protein deficiency could be prevented by Spirulina supplementation in growing rats.One-month old female rats were ...

ba0001pp90 | Bone development/growth and fracture repair | ECTS2013

The choice of fetal bovine serum influences the degree of spontaneous mineralization on silk fibroin scaffolds in 3D cell cultures

Paulsen Samantha , Vetsch Jolanda , Muller Ralph , Hofmann Sandra

Silk fibroin (SF) sponges are a promising scaffold material for tissue engineering due to their biocompatibility, mechanical properties, and ability to support calcium-phosphate formation in vitro. However, previous studies have shown that SF can mineralize spontaneously in the presence of culture media, which has a detrimental effect on experimental integrity when analyzing how cells deposit bone-like tissue in tissue engineering studies. In this study we analyzed th...

ba0001pp91 | Bone development/growth and fracture repair | ECTS2013

Pediatric differences in bone mineral density according to ethnic background in children: the Generation R Study

Medina-Gomez Carolina , Heppe Denise H M , Hofman Albert , Jaddoe Vincent , Uitterlinden Andre , Rivadeneira Fernando

Aim: Differences in fracture risk between ethnic groups have been documented. The basis for these differences is yet incomplete and the age at what ethnic differences appear is uncertain. Assessment of bone health in pediatric populations could bring insights on factors compromising bone accrual. We describe here differences in total body bone mineral density (TB-BMD) in a unique setting of children of the same age, measured with the same device (iDXA) different ethnic backgro...

ba0001pp92 | Bone development/growth and fracture repair | ECTS2013

Identifying scoliosis in population-based cohorts: development and validation of a novel method based on total body dual energy X-ray absorptiometry scans

Taylor Hilary , Harding Ian , Hutchinson John , Nelson Ian , Blom Ashley , Tobias Jon , Clark Emma

Background: Scoliosis is lateral curvature of the spine ≥10°, as measured on standing spinal radiographs. There are no published studies that have investigated determinants of scoliosis using a prospective cohort design, making the establishment of cause and effect difficult. Several large population-based cohorts exist throughout the world with a wide range of data already collected, and while spinal imaging with radiographs is not generally collected in these coho...

ba0001pp93 | Bone development/growth and fracture repair | ECTS2013

Smaller bones at aged 10 predicts scoliosis at aged 15: results from a population-based birth cohort

Taylor Hilary , Blom Ashley , Harding Ian , Hutchinson John , Nelson Ian , Tobias Jon , Clark Emma

Background: Scoliosis is lateral curvature of the spine, and adolescent idiopathic scoliosis (AIS) accounts for the majority of cases of scoliosis. One potential determinant of scoliosis that is of great interest is bone size and density. However, there are no published studies that have investigated determinants of scoliosis using a prospective cohort design making the establishment of cause and effect difficult.Methods: This study was based on the Avon...

ba0001pp94 | Bone development/growth and fracture repair | ECTS2013

The healing of fracture of mandible against the chronic nitrate intoxication

Aveticov David , Kostenko Vitaly , Neporada Karine , Lokes Ekaterina , Stavickiy Stanislav

Damages of bones of facial skeleton lay down 8% from all damages, fractures of mandible are 85–90% from it. There are a lot of factors, that make worse the process of reparative regeneration of bone. Using of nitric fertilizers lead to heighten earning of nitric oxide into organism. It makes negative influence on reparative regeneration of bones.There are dates of research of 40 rats line Vistar in this article. The goal of this study was to examine...

ba0001pp95 | Bone development/growth and fracture repair | ECTS2013

Osteometric parameters of mature rats mandible molars at implantation in the tibia of biogenic hydroxyapatite

Vladislav Luzin , Vitali Morozov , Helen Morozova

Traumatic injuries of the bones of various etiologies are accompanied not only a violation of their integrity, but also the development of a system osteopenic syndrome, which causes disorder neurohumoral regulation of the organism and has a negative effect on the structural and functional state of the skeletal tissues of other parts of the skeleton. The information concerning the characteristics of changes in the parameters of growth of molars of the mandible in the literature...

ba0001pp96 | Bone development/growth and fracture repair | ECTS2013

Macroelements composition of the dentin in rat's mandibular incisors with implanted biogenic hydroxyapatite into the tibia

Vladyslav Luzin , Vitaly Morozov , Dmitry Astrakhantsev , Pavel Golubkov , Marina Sokol

Objectives: The aim of the study was to establish features of macroelemental composition of the dentin in mature male rat’s mandibular incisors with biogenic hydroxyapatite, implanted in the proximal part of tibial shaft.Material and methods: One hundred and twenty-six mature male rats were divided into three groups: 1st group – intact animals, 2nd group – animals with the pit defect applied on the proximal part of tibial shaft (systematic...

ba0001pp97 | Bone development/growth and fracture repair | ECTS2013

Organometric parameters of rat's bones under the effect of toluene vapor

Skorobogatov A , Luzin V , Morozov V , Shutov Ye

Objectives: The aim of this study was establish features changes of organometric parameters of bones in mature male rats after a 60-day inhalation seed toluene.Materials and methods: For the experiment were selected 60 mature male rats were divided into two groups: 1st – intact rats, 2nd group – the rats that every day for 2 months to device for inhalation agents received a one-time inhalation of toluene exposure 4 h in 10 maximum permissible c...

ba0001pp98 | Bone development/growth and fracture repair | ECTS2013

Identification and characterization of a mesenchymal progenitor cell population involved in fracture healing

Matthews Brya , Grcevic Danka , Wang Liping , Hagiwara Yusuke , Adams Douglas , Kalajzic Ivo

Fracture healing is a multistep process that involves many cell lineages and is still not fully understood. We aimed to identify and characterize population of mesenchymal progenitor cells during its commitment within a fracture callus. To identify and trace cells in periosteum and bone marrow we used αSMA promoter-driven inducible Cre expression (αSMA-CreERT2) combined with a Cre-activated tdTomato reporter (Ai9) to generate αSMACre/Ai9 mice. Tibias, fixed with...